@article {1503, title = {Comparative HPTLC Fingerprinting Analysis of Yashtimadhu Medhya Rasayana in Different Media}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {September 2025}, pages = {1226-1234}, type = {Original Article}, chapter = {1226}, abstract = {

Background: Yashtimadhu (Glycyrrhiza glabra Linn.), classified as a Medhya Rasayana in Ayurveda, is traditionally administered with milk (Ksheera anupana) to enhance cognitive function. Despite these claims, the phytochemical basis and role of milk as an adjuvant in influencing the availability of active constituents like glycyrrhizin remain insufficiently explored. Objectives: To investigate the effect of different extraction media: water, milk and ksheerapaka on the glycyrrhizin content in Yashtimadhu using High-Performance Thin Layer Chromatography (HPTLC). Materials and Methods: TLC was initially conducted to screen phytochemical profiles of Yashtimadhu extracts (aqueous, milk, and ksheerapaka (milk-based decoction)). Based on these findings, HPTLC analysis was performed using methanolic extracts to identify and quantify glycyrrhizin, with comparison to a standard marker at 254 nm and 366 nm. Results: Glycyrrhizin was identified in all preparations, with Rf values ranging from 0.26 to 0.30. The milk extract (Y+M) showed the highest glycyrrhizin content (35,523.1 AU), followed by aqueous extract (31,329.1 AU) and ksheerapaka (28,220.9 AU). Conclusion: This study provides preliminary evidence supporting the Ayurvedic rationale of anupana. Milk appears to enhance glycyrrhizin availability, likely due to its amphipathic nature, highlighting its role in modulating herbal efficacy. Further in vivo and pharmacokinetic studies are warranted to validate these findings and optimize formulation strategies.

}, keywords = {Anupana, Glycyrrhiza glabra, Glycyrrhizin, HPTLC, Medhya Rasayana, Yashtimadhu}, doi = {10.5530/pres.20252333}, author = {Geeta Govindappa. Gadad and Sanskar Ankush Maral and Bhumika Sundar and Pragati Vinaykumar Kotur} } @article {1446, title = {Instrumental Characterization of Black Soot of Glycyrrhiza glabra: A Colouring Pigment for Eye Cosmetics}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {859-866}, type = {Original Article}, chapter = {859}, abstract = {

Background: A{\~n}janaṃ (collyrium) in Ayurveda is predominantly centered around its role in maintaining eye health rather than cosmetic use. This aligns with the traditional kohl/kajal application, prepared using herbs rich in anti-microbial properties, thus offering ocular benefits. Such kajals incorporate burnt black soot from plants to achieve the desired dark color. Analyzing this carbonized form is essential to confirm its therapeutic contribution among other ingredients. In this study, black soot particles obtained from Glycyrrhiza glabra (GG), a potent Ayurvedic herb known for promoting vision and addressing conditions such as dry eye disease, Cataract and conjunctivitis, were examined. Materials and Methods: The traditional method of preparing black soot was employed, involving scraping it from a copper plate after burning a wick dipped in GG{\textquoteright}s decoction seven times. Results: Physico-chemical analysis of the raw GG and its decoction met the API (Ayurvedic Pharmacopoeia of India) standards. The GG decoction pH, total solids and specific gravity were 5.3, 14.636\% and 1.048 respectively. FT-IR analysis revealed four bands corresponding to GG{\textquoteright}s methylene and methane groups, while XRD indicated the presence of cupric and cuprous oxide. The SEM-EDX results showed that the black soot contained 68.29\% carbon, 14.62\% oxygen, and 17.09\% copper. Conclusion: These findings suggest that even in its carbon form, black soot derived from GG using traditional methods retains active ingredients from the combustion of plant biomass. This underscores its potential therapeutic benefits in addition to its cosmetic purposes. The use of black soot from herbs in kajals can offer both cosmetic enhancement and ocular health advantages.

}, keywords = {Black soot, FT-IR, Herbal cosmetic, Kajal, SEM-EDX, XRD, Yashtimadhu}, doi = {10.5530/pres.20252172}, author = {Geeta Govindappa Gadad and Bhumika Sundar} }