@article {1460, title = {Chitin Derivative from Marine Source Artemia franciscana Cysts Inhibits Colorectal Adenocarcinoma Cells by Regulating Apoptotic Mediators}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {973-982}, type = {Original Article}, chapter = {973}, abstract = {

Background: Chitin is widely preferred in biomedical application due to its biocompatible and biodegradable nature. Naturally occurring chitin is a potential antimicrobial agent and plays a major role in degrading outer shell wall of the lower crustaceans. Naturally occurring sources of chitin are more suitable for oncological research. Objectives: In the present study, the chitin derivative isolated from marine source (Artemia franciscana cysts) is evaluated for its potential to act as a therapeutic target for colorectal adenocarcinoma. Materials and Methods: The FTIR and X-ray diffraction analysis of the isolated chitin has confirmed the presence of characteristic functional groups such as amide groups, hydroxyl groups and glycosidic linkages along with their material{\textquoteright}s crystalline structure. Results: The anticancer activity of extracted chitin derivative showed significant cytotoxic effect in this study which could be attributed to their positively charged functional groups. Further mRNA gene expression analysis has revealed that the chitin derivative also exhibited apoptotic potential against HT29 cell lines by upregulating caspase-3 and caspase-9 gene and by down regulation of B cell lymphoma-2 (Bcl-2) gene expressions. Conclusion: In conclusion the present study showed that the Artemia sp. cyst-mediated chitin derivative could be a potential anticancer agent against colorectal adenocarcinoma through their anti-proliferative activity and by activation of apoptotic pathway in HT29 cell lines

}, keywords = {Anti-cancer, Artemia, Chitin, Cyst shell, HT26}, doi = {10.5530/pres.20252138}, author = {Rajendran Poovazhagi and Subramani Thirunavukkarasu and Jeferson Stanley Santhosh and Kasi Lakshmanan and Anand Javee and Cecileya Jasmin Meshak Dhanashekaran and Narayanaswamy Radhakrishnan and Venugopal Vinod Prabhu and Kunnathur Murugesan Sakthivel and Rajan Radha Rasmi and Jiang-Shiou Hwang} } @article {1396, title = {Green Synthesis of Silver Nanoparticles from Secamone emetica: Characterization and Assessment of Antioxidant, Anti-Diabetic and Anti-Cancer Activities against MCF-7 Breast Cancer Cells}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {April 2025}, pages = {597-607}, type = {Original Article}, chapter = {597}, abstract = {

Background: Green-synthesized nanoparticles offer eco-friendly advantages over physical and chemical methods, utilizing plant extracts as natural capping, reducing and stabilizing agents. While Secamone emetica is renowned for its traditional medicinal applications, its potential in green nanoparticle synthesis remains unexplored, offering a promising avenue for research. Objectives: This study aimed to synthesize silver Nanoparticles (AgNPs) using aqueous leaf and stem extracts of Secamone emetica and evaluate their antioxidant, anti-diabetic and anti-cancer properties. Materials and Methods: The aqueous extracts of S. emetica Leaf (SL) and Stem (SS) were used for the green synthesis of AgNPs. The bio-reduction of silver ions by these extracts was validated through UV-visible spectroscopy, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR). Antioxidant activities were evaluated using DPPH+ and ABTS˙+ radical scavenging assays. The anti-diabetic potential was evaluated through α-amylase and α-glucosidase inhibition assays. Cytotoxic effects on MCF-7 breast cancer cell lines were assessed at 100 μg/mL concentration. Results: SL-AgNPs exhibited higher antioxidant activity compared to SS-AgNPs in both DPPH+ and ABTS˙+ assays. In anti-diabetic assays, SL-AgNPs demonstrated 70.1\% α-amylase inhibition and 68.4\% α-glucosidase inhibition, outperforming SS-AgNPs, which showed 59.3\% and 57.6\% inhibition, respectively. In the anti-cancer assay, SL-AgNPs exhibited a cytotoxicity of 62.5\% against MCF-7 cells, whereas SS-AgNPs showed 50.8\% inhibition. Conclusion: These findings highlight the potential of green-synthesized SL-AgNPs and SS-AgNPs as effective antioxidant, anti-diabetic and anti-cancer agents.

}, keywords = {Anti-cancer, Anti-diabetic, Antioxidant activity, cytotoxicity, Secamone emetica, Silver nanoparticles}, doi = {10.5530/pres.20252064}, author = {S. Shalini and N. Sudeepthi and DH. Geetha and R. Jayashre} }