@article {1020, title = {Isolation, Simultaneous Quantification of Taxifolin and Taxifolin-3-O-rhamnoside and Validation by RPHPLC}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {December 2021}, pages = {30-34}, type = {Original Article}, chapter = {30}, abstract = {

Background: Taxifolin (TA) is a flavonoid that has antioxidant, hepatoprotective, antialzheimer, anti-hyperglycemic, cardiovascular, anti-inflammatory, anti-psoriatic and antialzheimer properties. Taxifolin 3-O-rhamnoside (TAR), a glycoside of taxifolin, has antioxidant, anticonvulsant, anticancer, anti-inflammatory, and immunosuppressive effects. Objectives: The goal of this study is to separate TA and TAR from Smilax china Linn. rhizomes, as well as to develop and validate a technique for simultaneous measurement of TA and TAR. Materials and Methods: The hydro alcoholic extract of the rhizome of S. china yielded TA and TAR. HPLC system was fitted with a C18 column (shim-pack) of size 150 mm x 4.6 mm; 5μ, with suitable eluting mixture of methanol: water (90:10 v/v) at a flowing rate of 1 ml/min and at 254 nm wavelength for identification of peaks. Lab solution software was used to establish the quantification method for above-mentioned chemical compounds. Results: TAR and TA were separated using the proposed technique at Rt 2.917 and 3.924 min respectively. Over the range of 0.1-0.8 μg/ml, calibration curves were produced with a linear relationship R2 \> 0.9941 and 0.9963, respectively. The relative standard deviation was less than 2\%. The percentage recoveries were shown to be between 97 and 102.1. TA and TAR had detection limits of 0.156 and 0.077 μg/ml; quantification limits were 0.473 and 0.234 μg/ml respectively. The technique that was devised was simple, sensitive and specific. Conclusion: The newly designed RPHPLC technique has improved specificity, precision, and accuracy. The quality of S. china and other dietary supplements containing these two flavonoids may be successfully assessed by quantifying these two flavonoids and their glycosides.

}, keywords = {3, 3{\textquoteright}, 4{\textquoteright}-pentahydroxy flavanone, 5, 7, Astilbin, Dihydroqucertin, Tuberculosis, Validation.}, doi = {10.5530/pres.14.1.6}, url = {https://phcogres.com/fulltext/v14i1/105530pres1416.html}, author = {Subashini Subramanian and Shakila Ramachandran} } @article {993, title = {In-silico Molecular Docking Studies of some Isolated Phytochemicals from Biophytum veldkampii against Cyclooxygenase-II Enzyme and in vivo Anti-inflammatory Activity}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {October 2021}, pages = {192-198}, type = {Original Article}, chapter = {192}, abstract = {

Background: The current study estimate the anti-inflammatory activity of whole plant of Biophytum veldkampii on carageenan induced inflammation on rats compiled by molecular docking studies of phytocompounds from the plants with Cyclooxygenase II (PDB ID: 3LN1). Methods: In this research Biophytum veldkampii was subjected to extraction using methanol. In-vivo anti-inflammatory activity was assessed by using carageenan induced inflammation on rats and in-silico molecular docking studies was performed by using of Autodock 4.0. Results: The outcomes revealed that the methanolic extract has most prominenant anti-inflammatory activity at various doses. Among all the substances 2,6,3{\textquoteright},4{\textquoteright}-Tetrahydroxy-2-benzylcoumaranone revealed the most effective docking rating of -6.8, which is near to Diclofenac, i.e. - 6.9, ensuring that 2,6,3{\textquoteright},4{\textquoteright}-Tetrahydroxy-2-benzylcoumaranone has a strong binding fondness in between protein and ligand. Conclusion: From the results, a conclusion can be drawn that the anti-inflammatory activity of Biophytum veldkampii in both in vivo and in silico methods. This information sustains 2,6,3{\textquoteright},4{\textquoteright}-Tetrahydroxy-2-benzylcoumaranone to be a useful anti-inflammatory compound beneficial to future clinical studies.

}, keywords = {2, 3{\textquoteright}, 4{\textquoteright}-Tetrahydroxy-2 benzylcoumaranone, 6, Autodock4.0, Carageenan, Cyclooxygenase II, In-silico, Inflammation, Phytochemical}, doi = {10.5530/pres.13.4.11}, author = {Nagaraju Bandaru and D.S.N.B.K Prasanth and A.Rajasekhar Reddy and G.S.N Koteswara Rao and Kumar V.S Nemmani and Alla Narayana Rao} }