@article {1205, title = {Quality Control and Acute Toxicity Study of Habb-i-Hayat (Unani Formulation) in Albino Wistar Rats}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {98-109}, type = {Original Article}, chapter = {98}, abstract = {

Background: Several reports acknowledge that herbal products produce toxicities due to their poor quality. Hence, the worldwide promotion of traditional medicines is not at par due to a lack of quality control and safety aspects. Habb-i-Hayat, a Unani formulation, is used for the treatment of various bodily ailments. Aim: The present study was aimed at evaluating the quality control and acute toxicity of Habb-i-Hayat. Materials and Methods: The standardization and quality control study of Habb-i-Hayat, including organoleptic characters, physicochemical properties, qualitative phytochemical evaluation, microbial load, aflatoxins and heavy metals analysis, and HPTLC, were carried out. The acute toxicity study of the same was carried out at three dose levels following OECD guidelines 423. Results: The quality control findings of Habb-i-Hayat, including by weight variation, hardness, friability, moisture content, ash values, swelling index, etc., were found within the normal limits. The TBC and TFC were found within the permissible limits. The heavy metal analysis report showed that the heavy metals, including lead, cadmium, arsenic, and mercury, were present within the permissible limits. The number of peaks, Rf values, and area under the curve found in the HPTLC study may be used for identification and quality of Habb-i- Hayat. The toxicity data of Habb-i-Hayat revealed that it produces some serious toxic control effects including death at higher doses. The LD50 of Habb-i-Hayat was found to be 2500 mg/kg b. w. for rats. Conclusion: The standardization and quality control reports of Habb-i-Hayat have suggested that the preparation, drying, and storage of Habb-i-Hayat followed standard operating procedures. Moreover, the findings of standardization and quality control of Habb-i-Hayat may be referred in future. The acute toxicity profile of Habb-i-Hayat has confirmed that this important Unani formulation may be safely used at therapeutic dose levels.

}, keywords = {Acute toxicity, Habb-i-Hayat, Quality control, Standardization, Unani formulation}, doi = {10.5530/pres.16.1.12}, author = {Ifra Qayoom and Athar Parvez Ansari and Ansari Huzaifa and Mohd Musaib Bhat and Bazilah Majeed Reshi and Pankaj Goswami and Noman Anwar} } @article {110, title = {Acute and Subchronic Oral Toxicity Studies of Majun Brahmi and Itrifal Muqawwi Dimagh (Traditional Unani Formulations) in Rats}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {May 2020}, pages = {169-175}, type = {Original Article}, chapter = {169}, abstract = {

Background: Majun Brahmi (MB) and Itrifal Muqawwi Dimagh (IMD) are polyherbal Unani formulations traditionally used for the treatment of mild cognitive impairment and memory enhancement. Although they are widely used, there is no particular scientific evidence regarding the toxicity of these medicines. Objective: The present study was undertaken to evaluate the safety of MB and IMD polyherbal Unani formulation. Materials and Methods: Wistar albino rats of both genders were treated with three doses of each drug {\textendash} MB and IMD, i.e., 513.88, 1027.77, and 2055.54 mg/kg body weight (bwt) orally for 14 days for acute and 90 days for subchronic oral toxicity studies. At the end of the study, bwt gain, hematology, clinical biochemistry, and histopathological examination were performed. Although no significant changes in biochemical parameters occurred at the highest dose, mild damage to the liver, kidney, and spleen was observed. Results: At the lowest dose and at the dose of 1027.77 mg/kg bwt (therapeutic effective dose [TED]) of both the drugs, no abnormalities were observed in the biochemical and hematological parameters as well as histopathological findings. Conclusion: None of the rats exhibited apparent toxicity or mortality. The results of the present study provide evidence that both the formulations are not toxic at the TED dose and hence are clinically safe.

}, keywords = {Acute toxicity studies, Itrifal Muqawwi Dimagh, Majun Brahmi, Subchronic oral toxicity, Unani formulation}, doi = {10.4103/pr.pr_93_19}, author = {Monika Kumari and Amjad Saifi and Mahmood A. Khan and Vinod Kumar Arora and Yasmeen Shamsi and Sumita Halder and Rafat Sultana Ahmed} }