@article {1217, title = {A Two-Year Comparative Study of the Anti-dermatophytic Activity of Thuja orientalis Essential Oil Stored at Various Temperatures}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {191-196}, type = {Original Article}, chapter = {191}, abstract = {

Background: Thuja orientalis generally recognized as Chinese arborvitae, placed under the family Cupressaceae is a commonly available medicinal plant in India. Objectives: In present research work Thuja leaves essential oil stored in three temperatures for two-years storage periods was evaluated against fungi causing ring worm infection in man. Materials and Methods: Thuja oil was hydro-distilled and stored for 24 months in airtight amber glass bottles at three distinct temperatures. MIC was determined through semi solid agar diffusion method containing BHIA medium. Anti-dermtophytic activity of stored oil was examined in triplets after every fourth month of storage up to 2 years. Results: Oil stored in refrigerator was found to be more stable up to two year of storage time. No activity changes were observed in this condition against all test fungi. While variable temperature condition of room temperature was found less suitable for the storage of oil. MIC against Candida albicans was increased 40\% after 2 years (\>3.5 μL/mL) and 66\% against Microsporum fulvum. Oil stored at 30{\textordmasculine}C temperature condition was found more stable upto IV testing. Slightly changes were observed during Vth testing against C. albicans, M. fulvum and M. canis. Conclusion: Present study concluded that essential oil of Thuja put away in chilliness condition can reserved their quality and properties of antifungal behaviour for long time. Accordingly, putting away of volatile oil ointment items in coolness (beneath 8{\textordmasculine}C) condition is best guidelines for long term use of therapeutic items.

}, keywords = {MIC., Microsporum, Thuja, Trichophyton, Volatile oil}, doi = {10.5530/pres.16.1.24}, author = {Neetu Jain and Seema Bhadauria} } @article {930, title = {Secondary Metabolites and Bioactivities of Myrtus communis}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {January 2011}, pages = {325-329}, type = {Original Article}, chapter = {325}, abstract = {

Background: Myrtus species are characterized by the presence of phenolic acids, flavonoids, tannins, volatile oils and fatty acids. They are remedies for variety of ailments. This study therefore investigated medicinal effects of Myrtus communis L. Methods: Bioactivity studies of Myrtus communis L. leaves were carried out on volatile oil, 7\% methanol and aqueous extracts and the isolated compounds myricetin 3-O-β-glucopyranoside, myricetin 3-O-∝-rhamnopyranoside and gallic acid. Results: Determination of the median lethal dose (LD 50 ) revealed that the volatile oil, alcoholic and aqueous extracts were practically nontoxic and highly safe as no lethality was observed. The tested materials (volatile oil, alcoholic and aqueous extracts, myricetin 3-O-β-glucopyranoside, myricetin 3-O-∝-rhamnopyranoside and gallic acid) showed significant antihyperglycemic, anti-inflammatory and antinociceptive effects as compared with control groups and reference drugs. Conclusion : Administration of extracts of M. communis leaves could be safe at the dose used in this study.

}, keywords = {Anti-inflammatory, Antihyperglycemic, Antinociceptive, LD 50, Myrtus communis, Volatile oil}, doi = {10.4103/0974-8490.75449}, author = {Mahmoud I Nassar and El-Sayed A Aboutabl and Rania F Ahmed and Ezzel-Din A EL-Khrisy and Khaled M Ibrahim and Amany A Sleem} }