@article {1020, title = {Isolation, Simultaneous Quantification of Taxifolin and Taxifolin-3-O-rhamnoside and Validation by RPHPLC}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {December 2021}, pages = {30-34}, type = {Original Article}, chapter = {30}, abstract = {

Background: Taxifolin (TA) is a flavonoid that has antioxidant, hepatoprotective, antialzheimer, anti-hyperglycemic, cardiovascular, anti-inflammatory, anti-psoriatic and antialzheimer properties. Taxifolin 3-O-rhamnoside (TAR), a glycoside of taxifolin, has antioxidant, anticonvulsant, anticancer, anti-inflammatory, and immunosuppressive effects. Objectives: The goal of this study is to separate TA and TAR from Smilax china Linn. rhizomes, as well as to develop and validate a technique for simultaneous measurement of TA and TAR. Materials and Methods: The hydro alcoholic extract of the rhizome of S. china yielded TA and TAR. HPLC system was fitted with a C18 column (shim-pack) of size 150 mm x 4.6 mm; 5μ, with suitable eluting mixture of methanol: water (90:10 v/v) at a flowing rate of 1 ml/min and at 254 nm wavelength for identification of peaks. Lab solution software was used to establish the quantification method for above-mentioned chemical compounds. Results: TAR and TA were separated using the proposed technique at Rt 2.917 and 3.924 min respectively. Over the range of 0.1-0.8 μg/ml, calibration curves were produced with a linear relationship R2 \> 0.9941 and 0.9963, respectively. The relative standard deviation was less than 2\%. The percentage recoveries were shown to be between 97 and 102.1. TA and TAR had detection limits of 0.156 and 0.077 μg/ml; quantification limits were 0.473 and 0.234 μg/ml respectively. The technique that was devised was simple, sensitive and specific. Conclusion: The newly designed RPHPLC technique has improved specificity, precision, and accuracy. The quality of S. china and other dietary supplements containing these two flavonoids may be successfully assessed by quantifying these two flavonoids and their glycosides.

}, keywords = {3, 3{\textquoteright}, 4{\textquoteright}-pentahydroxy flavanone, 5, 7, Astilbin, Dihydroqucertin, Tuberculosis, Validation.}, doi = {10.5530/pres.14.1.6}, url = {https://phcogres.com/fulltext/v14i1/105530pres1416.html}, author = {Subashini Subramanian and Shakila Ramachandran} } @article {921, title = {Methanolic Extracts of Aloe Secundiflora Engl. Inhibits in vitro Growth of Tuberculosis and Diarrhea-causing Bacteria}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, pages = {95-99}, chapter = {95}, abstract = {

Background: The emergence of resistance to antimicrobials by pathogens has reached crisis levels, calling for identification of alternative means to combat diseases. Objective: To determine antimicrobial activity of crude methanolic extract of Aloe secundiflora Engl. from Lake Victoria region of Kenya. Materials and Methods: Extract was tested against four strains of mycobacteria (Mycobacterium tuberculosis, M. kansasii, M. fortuitum and M. smegmatis), Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and a fungus Candida albicans. activity of the extract was determined using BACTECTM MGITTM 960 system. General antibacterial and antifungal activity was determined using standard procedures: zones of inhibition, Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal/Fungicidal Concentrations (MBCs/MFCs). Results: The extract was potent against M. fortuitum, M. smegmatis and M. kansasii where it completely inhibited growth (Zero growth units (GUs)) in all the extract concentrations used. It gave strong antimycobacterial activity (157 GUs) against M. tuberculosis. It showed strong antimicrobial activity (P<=0.05), giving inhibition zones >=9.00 mm against most microorganisms, such as P. aeruginosa (MIC 9.375 mg mL-1and MBC of 18.75 mg mL-1), E. coli (both MIC and MBC of 18.75 mg mL-1), S. aureus and S. typhi (both with MIC and MBC of 37.5 mg mL-1). Preliminary phytochemistry revealed presence of terpenoids, flavonoids and tannins. Conclusion: The data suggests that Aloe secundiflora could be a rich source of antimicrobial agents. The result gives scientific backing to its use by the local people of Lake Victoria region of Kenya, in the management of conditions associated with the tested microorganisms.

}, keywords = {Aloe secundiflora Engl., BACTEC{\texttrademark} MGIT{\texttrademark} 960, growth units, Lake Victoria region, Tuberculosis}, doi = {10.4103/0974-8490.81956}, author = {Richard M. Mariita and John A. Orodho and Paul O. Okemo and Claude Kirimuhuzya and Joseph N. Otieno and Joseph J. Magadula} }