@article {1355, title = {Exploring the Glycosidic Compounds in Ipomoea sagittifolia Stems: A High-Performance Liquid Chromatography Approach}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {162-172}, type = {Original Article}, chapter = {162}, abstract = {

Background: The abstract provides a comprehensive overview of the study on secondary metabolites from Ipomoea sagittifolia, emphasizing the extraction, isolation and characterization processes. Secondary metabolites, including alkaloids, flavonoids, phenols and glycosides, were identified and analyzed using various analytical techniques. Materials and Methods: Ethanol extraction followed by Soxhlet apparatus yielded a crude extract, subsequently purified through column chromatography using a solvent mixture of n-butanol, glacial acetic acid and water. Thin Layer Chromatography (TLC) confirmed the presence of compounds across 108 eluted fractions, further validated by UV-vis spectroscopy revealing absorption peaks characteristic of glycosides at 288 nm. Results: Fourier Transform Infrared (FTIR) spectroscopy highlighted functional groups such as alcohols, phenols, carboxylic acids and ketones, typical of glycoside structures. High-Performance Liquid Chromatography (HPLC) confirmed the presence of cardiac glycosides through retention time analysis, aligning closely with Scillaren A standards. Conclusion: These findings underscore the pharmacological potential of Ipomoea sagittifolia in traditional medicine and pharmaceutical applications, supported by its diverse secondary metabolite profile. Future research could delve deeper into bioactivity assays to elucidate specific therapeutic properties linked to these metabolites, thereby enhancing its relevance in healthcare and biotechnological innovations.

}, keywords = {Bioactive, Elucidation, High performance thin layer chromatography, Ipomea}, doi = {10.5530/pres.20251964}, author = {Raveesha Peeriga and Krishnaveni Manubolu and Jayaprakash Somasundaram and Sheikdawood Asanar Mohamed Shiek Arabath and Shanthi Petchimuthu and Sri Naga Joshitha Manepalli and Vinay Kumar Maddela and Kavya Makireddy} } @article {800, title = {Antimicrobial Properties, Antioxidant Activity and Bioactive Compounds from Six wild Edible Mushrooms of Western Ghats of Karnataka, India}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {May 2010}, pages = {107-112}, type = {Original Article}, chapter = {107}, abstract = {

Methanolic extracts of 6 wild edible mushrooms isolated from the Western Ghats of Karnataka were used in this study. Among the isolates (Lycoperdon perlatum, Cantharellus cibarius, Clavaria vermiculris, Ramaria formosa, Marasmius oreades, Pleurotus pulmonarius), only 4 showed satisfactory results. Quantitative analysis of bioactive components revealed that total phenols are the major bioactive component found in extracts of isolates expressed as mg of GAE per gram of fruit body, which ranged from 3.20 {\textpm} 0.05 mg/mL to 6.25 {\textpm} 0.08 mg/mL. Average concentration of flavonoid ranged from 0.40 {\textpm} 0.052 mg/mL to 2.54 {\textpm} 0.08 mg/mL; followed by very small concentration of ascorbic acid (range, 0.06 {\textpm} 0.01 mg/mL to 0.16 {\textpm} 0.01 mg/mL) in all the isolates. All the isolates showed high phenol and flavonoid content, but ascorbic acid content was found in traces. Antioxidant efficiency by inhibitory concentration on 1,1-Diphenly-2-picrylhydrazyl (DPPH) was found significant when compared to standard antioxidant like Buthylated hydroxyanisol (BHA). The concentration (IC 50 ) ranged from 0.94 {\textpm} 0.27 mg/mL to 7.57 {\textpm} 0.21 mg/mL. Determination of antimicrobial activity profile of all the isolates tested against a panel of standard pathogenic bacteria and fungi indicated that the concentrations of bioactive components directly influence the antimicrobial capability of the isolates. Agar diffusion assay showed considerable activity against all bacteria. Minimum inhibitory concentration values of the extracts of 4 isolates showed that they are also active even in least concentrations. These results are discussed in relation to therapeutic value of the studied mushrooms.

}, keywords = {Agar diffusion assay, Antioxidant, Bioactive, DPPH mushrooms, in-vitro, Western Ghats}, doi = {10.4103/0974-8490.62953}, author = {Ch. Ramesh and Manohar G Pattar} }