@article {1275, title = {HPTLC Method Development and Validation for Simultaneous Quantification of Purpurin and Alizarin in Rubia cordifolia L. Roots and their Marketed Preparations}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {June 2024}, pages = {652-661}, type = {Original Article}, chapter = {652}, abstract = {

Background: Rubia cordifolia L. a spiky perennial climber of Rubiaceae family, is a rich reservoir of anthraquinone containing compounds purpurin and alizarin. It emerges as a versatile powerhouse possessing diverse pharmacological properties which includes antibacterial, antitumor, antioxidant, antiplatelet, potent blood purifier, diuretic, anti-stress, neuroprotective, anti-mutagenic, anticancer, anti-inflammatory, antidepressant, antidiabetic and vasodilation. Thereby it offers a holistic approach for disease prevention through its pharmacological properties. Objectives: However due to lack of standardization for R. cordifolia roots and its derived marketed herbal products, its quality control remains a daunting task..The present study aims to develop a simple, novel, reliable and specific HPTLC method for quantifying purpurin and alizarin in R. cordifolia roots as well as in its marketed preparations. Materials and Methods: The separation and quantification of purpurin and alizarin has been carried out on HPTLC plates pre-coated with Silica gel 60 F254 as stationary phase and toluene, ethyl acetate, glacial acetic acid (6:3.5:0.5; v/v/v) as mobile phase. Determination and quantification were performed by densitometric scanning at 254 nm. Results: The developed method gave compact spots at Rf 0.57{\textpm}0.007 for purpurin and 0.73{\textpm}0.008 for alizarin. The method was validated as per International Council for Harmonization Q2(R1) guidelines for specificity, precision, robustness, accuracy and recovery. Linearity range for purpurin and alizarin was 100-2000 ng/spot, which showed good regression coefficient for purpurin and alizarin; R{\texttwosuperior}=0.9967 and R{\texttwosuperior}=0.9941 at 254 nm respectively. The LOD and LOQ for validated marker compounds purpurin and alizarin at 254 nm were found as 23.87{\textpm}0.52, 71.61{\textpm}0.84 ng/spot and 18.36{\textpm}0.72, 55.08{\textpm}0.26 ng/spot respectively. The content of purpurin and alizarin in R. cordifolia plant extract was found as 17.960{\textpm}0.658, 34.645{\textpm}1.153 μg/mg, marketed preparation A, contains 21.741{\textpm}0.746 and 6.824{\textpm}0.485 μg/mg while marketed preparation B, contains 18.289{\textpm}1.014 and 3.031{\textpm}0.234 μg/mg of the sample. Conclusion: The developed method was found to be simple, precise, accurate, economical and convenient for rapid screening of bioactive marker purpurin and alizarin present in methanolic extracts of R. cordifolia roots and its marketed preparations.

}, keywords = {Alizarin, Densitometry, HPTLC, Method validation, Purpurin, Rubia cordifolia L.}, doi = {10.5530/pres.16.3.76}, author = {Rizwan Ahmad and Zeeshan Fatima and Sadath Ali and Suneela Dhaneshwar and Sayeed Ahmad} } @article {100, title = {High-Performance Thin-Layer Chromatography Estimation of Boeravinone-B in Boerhavia diffusa L. and its Polyherbal Dosage Form (Capsule)}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {August 2019}, pages = {267-272}, chapter = {267}, abstract = {

Background: Qualitative and quantitative marker estimation is the quality basis of Ayurvedic pharmaceuticals. Boerhavia diffusa is a widely used herb in the Indian system of medicine. Objective: Estimation of boeravinone-B in hydroalcoholic extract of raw drug (ingredient) as well as its poly-herbal formulation to authenticate ingredients ratio in finished product by high-performance thin layer chromatography (HPTLC) method. Materials and Methods: Based on planar chromatographic method (HPTLC) with optimized mobile phase toluene: ethyl acetate: formic acid: methanol (5:3:1:1 v/v). Further densitometry and marker quantification of developed plate were carried out at ultraviolet 254 nm. Results: Presented in the form of comparative characteristic HPTLC fingerprints and densitograms with well-resolved prominent bands for boeravinone-B at retardation factor (Rf) {\textendash} 0.87 in both the samples. The linear regression by calibration plots revealed good linear relationship with 0.99953 with standard deviation 0.74\% with respect to the area in the concentration range of 200{\textendash}1000 ng/spot. Statistical analysis proves that the developed quantification method is reproducible and selective. Findings showed the presence of 0.055\% and 0.012\% w/w boeravinone-B in hydroalcoholic extract of B. diffusa and its polyherbal formulation, respectively, at Rf 0.87 under λ 254 nm. Conclusion: The study established fast, simple, precise, and cost-effective methods for qualitative and quantitative studies of Ayurvedic raw drugs, and polyherbal formulations consist of B. diffusa. It estimated boeravinone-B in raw drug (ingredient) as well as in its polyherbal formulation. The study qualitatively and quantitatively authenticated the presence and ratio of B. diffusa in polyherbal dosage form (capsule). Moreover, it suggested that the plant contains rich amount of boeravinone-B; this may be directed in selection of genuine plant species in formulation development as well as in standardization.

}, keywords = {Boeravinone-B, Boerhavia diffusa, Densitometry, High-performance thin-layer chromatography, Polyherbal formulation.}, doi = {10.4103/pr.pr_161_18}, author = {Arjun Singh and Himanshu Sharma and Ravinder Singh and Narayanam Srikanth} } @article {422, title = {High Performance Thin layer Chromatography: Densitometry Method for Determination of Rubraxanthone in the Stem Bark Extract of Garcinia cowa Roxb}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {July 2017}, pages = {230-233}, type = {Original Article}, chapter = {230}, abstract = {

Context: Garcinia cowa is a medicinal plant widely grown in Southeast Asia and tropical countries. Various parts of this plant have been used in traditional folk medicine. The bark, latex, and root have been used as an antipyretic agent, while fruit and leaves have been used as an expectorant, for indigestion and improvement of blood circulation. Aims: This study aims to determine the concentration of rubraxanthone found in ethyl acetate extract of the stem bark of G. cowa by the high-performance thin-layer chromatography (HPTLC). Materials and Methods: HPTLC method was performed on precoated silica gel G 60 F254 plates using an HPTLC system with a developed mobile-phase system of chloroform: ethyl acetate: methanol: formic acid (86:6:3:5). A volume of 5 μL of standard and sample solutions was applied to the chromatographic plates. The plates were developed in saturated mode of twin trough chamber at room temperature. The method was validated based on linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), and specificity. The spots were observed at ultraviolet 243 nm. Results: The linearity of rubraxanthone was obtained between 52.5 and 157.5 ppm/spot. The LOD and LOQ were found to be 4.03 and 13.42 ppm/spot, respectively. Conclusion: The proposed method showed good linearity, precision, accuracy, and high sensitivity. Therefore, it may be applied for the quantification of rubraxanthone in ethyl acetate extract of the stem bark of G. cowa.

}, keywords = {Chromatography, Densitometry, Garcinia cowa Roxb., High-performance thin-layer chromatography, Rubraxanthone, Validation}, doi = {10.4103/pr.pr_144_16}, author = {Dachriyanus Hamidi and Hilyatul Aulia and Meri Susanti} }