@article {99, title = {Evaluation of Biochemical Changes in Diabetic Rats Treated with Aegle marmelos (L.) Methanolic Leaf Extract}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {May 2020}, pages = {127-130}, type = {Original Article}, chapter = {127}, abstract = {

Background: Aegle marmelos (L.) Correa is a widely found plant in India as well as in South Asia. For more than several centuries, it is being widely used for its medicinal properties. Objective: The objective of this study was to evaluate the biochemical changes in alloxan-induced diabetic rats treated with methanolic leaf extracts of A. marmelos. Materials and Methods: Six treatment groups, namely control, diseased, standard (glimepiride), low dose (100 mg/kg), medium dose (250 mg/kg), and high dose (500 mg/kg) of methanolic leaf extracts, were used in the study. The biochemical effects were evaluated by the determination of bodyweight, blood glucose, serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), total proteins, serum albumin, serum creatinine, and alkaline phosphatase. Results: A significant increase in the bodyweight of the animals was observed in the high-dose treated animals (350.0 {\textpm} 6.15) when compared to the diseased group animals (241.0 {\textpm} 7.23). A significant decrease in the blood glucose, SGOT, and SGPT levels was observed in the high-dose treated animals (142.3 {\textpm} 20.52, 71.6 {\textpm} 4.8, and 24.5 {\textpm} 2.42) when compared to the diseased group animals (292.8 {\textpm} 29.34, 146.3 {\textpm} 11.12, and 74.5 {\textpm} 2.88), respectively. Similarly, total proteins, serum albumin, serum creatinine, and alkaline phosphatase levels of the high-dose treated animals were also significantly decreased (6.1 {\textpm} 0.26, 4.2 {\textpm} 0.22, 0.4 {\textpm} 0.18, and 109.2 {\textpm} 14.58) when compared to the diseased group animals (9.7 {\textpm} 0.27, 5.4 {\textpm} 0.26, 1.0 {\textpm} 0.22, and 257.2 {\textpm} 8.22), respectively. Conclusion: Through the biochemical changes, it is evident that the high dose of methanolic leaf extract of A. marmelos can be used in the treatment of diabetes and its complications.

}, keywords = {Aegle marmelos, Alloxan, Bael, Diabetes, Rutaceae}, doi = {10.4103/pr.pr_53_19}, author = {Ravi Babu Birudu and Padmavathi Pamulapati and Sathish Kumar Manoharan} } @article {162, title = {Evaluation of Cytotoxicity and Antioxidant Potential of Bael Leaf (Aegle marmelos) on Human Hepatocellular Carcinoma Cell Line}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {August 2020}, pages = {267-271}, type = {Original Article}, chapter = {267}, abstract = {

Background: Aegle marmelos (Bael) is one of the important medicinal plants for curing human diseases. Nevertheless, the information about the cytotoxic and antioxidant effects of Bael leaf is not much. Objectives: The objective of this study is to evaluate the cytotoxicity and antioxidant activity of Bael leaf in human hepatocellular carcinoma (HepG2) cell lines. Materials and Methods: Bael leaves extract was prepared by 80\% (v/v) aqueous ethanol and measured for total phenolic compound and antioxidant capacity. The ability of ethanolic Bael leaf extract to inhibit hepatic cancer cells (HepG2 cell) growth was evaluated by determining the percentages of cell viability. The levels of intracellular reactive-oxygen species (ROS) and lipid peroxidation were determined. Results: Bael leaf extract demonstrated the potentiality to inhibit the cancer cell growth with a 50\% inhibitory concentration (IC50) at 50 μg/ml and 72 μg/ml after 24 h and 48 h incubated times, respectively. The total phenolic content and antioxidant capacity of ethanolic extract were 218.33 {\textpm} 43.81 mg gallic acid equivalent/g of dry extract and 33.62 {\textpm} 3.07 mg Trolox equivalent antioxidant capacity/g of dry extract, respectively. At the lowest concentration of Bael leaf extract, it was found that Bael leaf extract shows the antioxidant activity by the ability to lowering the level of ROS and malondialdehyde in HepG2 cell. Conclusion: Bael leaf has a high antioxidant component, which is beneficial and can be developed as new therapeutic uses. However, further studies on the benefits of Bael leaf should be performed for better realizing and effective use soon.

}, keywords = {Antioxidant, Bael, cytotoxicity, Human hepatocellular carcinoma cell line, Malondialdehyde, Reactive-oxygen species}, doi = {10.4103/pr.pr_15_20}, author = {Kanokwan Kulprachakarn and Sakaewan Ounjaijean and Somdet Srichairatanakool and Duangta Kanjanapothi} }