@article {1320, title = {High-Performance Thin Layer Chromatography (HPTLC) Analysis for the Quantification of Constituents in Ficus religiosa}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {929-934}, type = {Original Article}, chapter = {929}, abstract = {

Background: Ficus religiosa, revered for its medicinal properties in traditional medicine, has drawn scientific interest due to its diverse chemical composition. This study aimed to develop a densitometric High-Performance Thin Layer Chromatography (HPTLC) technique to estimate phenolic compounds present in the hydroalcoholic extract of dried root bark of Ficus religiosa. Materials and Methods: This study utilized HPTLC to identify the constituents within Ficus religiosa root bark. The isolated constituent was evaluated by various analytical methods like phytochemical tests, UV, HPTLC etc. Subsequently, the HPTLC method underwent rigorous validation, confirming its reliability and accuracy in separating and quantifying the constituents within the root bark of Ficus religiosa. Results: Isolation of phytoconstituents was done by column chromatographic technique using toluene: ethyl acetate: methanol (3:3:2). The isolated component was confirmed as a flavonoid moiety by various chemical tests and by analytical evaluation. Also, HPTLC analysis of the isolate revealed the compound was Quercetin a flavonoid moiety or its derivative. Conclusion: These findings underscore the presence of main phytoconstituents in Ficus religiosa root bark which is responsible for pharmacological effects and offers insights into its application in both traditional and contemporary medicinal practices.

}, keywords = {Ficus religiosa, Flavonoids, HPTLC, Quantification, Validation}, doi = {10.5530/pres.16.4.106}, author = {Jyothisree Gangadharan and Umadevi Sankararajan} } @article {745, title = {The study of aqueous extract of Ficus religiosa Linn. on cytokine TNF-α in type 2 diabetic rats}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, month = {April 2011}, pages = {30-34}, type = {Original Article}, chapter = {30}, abstract = {

Background:\ Chronic systemic inflammation is an early process in pathogenesis of type 2 diabetes. Hence the present study was aimed to investigate the effect of traditionally known plant\ Ficus religiosa\ on elevated glucose and inflammatory marker namely tumor necrosis factor (TNF)-α in type 2 diabetic rats.\ Methods:\ Type 2 diabetes was induced by administering streptozotocin (90 mg/kg, i.p.) in neonatal rat model. Aqueous extract of\ F. religiosa\ at a dose of 100 and 200 mg/kg was given orally to desired group of animals for a period of 4 weeks. After 4 weeks of drug treatment, parameters such as fasting blood glucose, postprandial blood glucose and TNF-α in serum were analyzed.\ Results:\ Aqueous extract of\ F. religiosa\ at both dose levels i.e., 100 and 200 mg/kg decreased the elevated glucose and TNF-α in type 2 diabetic rats. The extract at 200 mg/kg had more pronounced effect.\ Conclusion:\ Modulation of cytokine TNF-α by the aqueous extract of\ F. religiosa\ indicates that the anti-inflammatory and immunomodulatory property of the plant is related with its potential anti-diabetic activity.

}, keywords = {Ficus religiosa, Inflammation, TNF-α, type 2 diabetes}, doi = {10.4103/0974-8490.79112}, author = {H Kirana and MV Jali and BP Srinivasan} }