@article {707, title = {In-vitro cytotoxicity Study of Methanolic Fraction from Ajuga Bracteosa Wall ex. benth on MCF-7 Breast Adenocarcinoma and Hep-2 larynx Carcinoma Cell Lines}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {December,2013}, pages = {87-91}, type = {Original Article}, chapter = {87}, abstract = {

Objective: Ajuga bracteosa Wall ex Benth (Labiatae) is popularly known in India as "Neelkanthi." A decoction of the leaves, flowers, and barks is used in India for the treatment of cancer including diabetes, malaria, and inflammation etc. The main objective of this study is to investigate the cytotoxic potential of Ajuga bracteosa. Materials and Methods: Successive solvent extraction of Ajuga bracteosa in petroleum ether, methanol, and water extracts was done. These extracts were tested against human breast adenocarcinoma (MCF-7) and larynx carcinoma (Hep-2) tumor cell lines, using the thiazolyl blue test (MTT) assay. Results: The methanolic fraction of Ajuga bracteosa had shown the significant results against MCF-7 and Hep-2 tumor cell lines. The methanolic, petroleum ether and aqueous extract from Ajuga bracteosa, presented an IC50 value at 24 hr of 10, 65, 70 μg/ml and 5, 30, 15 μg/ml on MCF-7 and Hep-2 cells, respectively. Steroids compounds namely β-sitosterol and unknown constituents were identified in the most active methanol extract of Ajuga bracteosa wall ex Benth. These known and unknown compounds exhibited cytotoxic potential against MCF-7 and Hep-2 cancer cells. Conclusion: Among all the tested extracts, methanolic extract can be considered as potential sources of anti-cancer compounds. Further studies are necessary for more extensive biological evaluations.

}, keywords = {Ajuga bracteosa, Cytotoxicity studies, Hep-2, MCF-7, MTT assay}, doi = {10.4103/0974-8490.122923}, author = {Akiriti Pal and Fedelic Ashish Toppo and Pradeep K Chaurasiya and Pradeep K Singour and Rajesh S Pawar} } @article {717, title = {In vitro antiproliferative activity of Annona reticulata roots on human cancer cell lines}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, month = {April 2011}, pages = {09-12}, type = {Original Article}, chapter = {09}, abstract = {

Background:\ The phytochemical and pharmacological activities of\ Annona reticulata\ components suggest a wide range of clinical application in lieu of cancer chemotherapy.\ Materials and Methods:\ Ethanol and aqueous extracts of roots of\ Annona reticulata\ Linn were studied for their\ in vitro\ antiproliferative activity on\ A-549\ (human lung carcinoma),\ K-562\ (human chronic myelogenous leukemia bone marrow), HeLa\ (human cervix) and\ MDA-MB\ (human adenocarcinoma mammary gland) cancer cell lines by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay.\ Results:\ The ethanol extract exhibited a prominent inhibitory effect against\ A-549, K-562, HeLa\ and\ MDA-MB\ human cancer cell lines at a concentration range between 10 and 40 μg/ml, whereas the aqueous extract showed a lower activity at the same concentration. Simultaneously, the effect of the ethanol extract toward the inhibition of\ Vero\ cell line proliferation was lower in comparison with the cancer cell lines.\ Conclusion:\ The significant antiproliferative activity of the ethanol extract of\ Annona reticulata\ roots against\ A-549, K-562, HeLa\ and\ MDA-MB\ human cancer cell lines may be attributed toward the collective presence of acetogenins, alkaloids and lower inhibitory effect on\ Vero\ cell line, which suggests\ Annona reticulata\ be used as a chemopreventive agent in cancer therapy.

}, keywords = {A-549, Annona reticulata, Antiproliferative activity, HeLaand MDA-MBhuman cancer cell lines, K-562, MTT assay}, doi = {10.4103/0974-8490.79109}, author = {HM Suresh and B Shivakumar and K Hemalatha and SS Heroor and DS Hugar and K.R.S Sambasiva Rao} } @article {861, title = {Anti-leukemia Activity of Methanolic Extracts of Lantana camara}, journal = {Pharmacognosy Research}, volume = {1}, year = {2009}, month = {January 2010 }, pages = {274-279}, type = {Original Article}, chapter = {274}, abstract = {

Anticancer effect of Lantana camara{\textquoteright}s root and leaf extracts against Jurkat leukemia cell line was investigated by MTT assay. These extracts had statistically similar antineoplastic property (root IC50, 328.36 {\textpm} 53.08 μg/ml; leaf, 394.41 {\textpm} 99.73 μg/ml; p \> 0.1, n = 3), averagely 1/10 times as activity as carboplatin (IC50 34.83 {\textpm} 3.60 μg/ml; p \< 0.05, n = 3). Decreasing cytotoxicity at higher concentrations implied the existence of cytoprotective compounds. Morphological examinations indicated apoptosis induction as the mechanism of activity on Jurkat cells. In conclusion, L. camara{\textquoteright}s root and leaf extracts might be subjects for further fractionation and identification to find new anticancer agents.

}, keywords = {anticancer, Cell Culture, Jurkat Cell Line, Lantana camara, MTT assay}, doi = {Nil}, author = {Badakhshan Mahdi Pour and Sreenivasan Sasidharan and Rameshwar Naidu Jegathambigai and Ramanathan Surash} }