@article {674, title = {Seasonal Variation in Content of Camptothecin from the Bark of Nothapodytes Nimmoniana (Grah.) Mabb., using HPLC Analysis}, journal = {Pharmacognosy Research}, volume = {5}, year = {2013}, month = {May 2013}, pages = {219-223 }, type = {Original Article }, chapter = {219}, abstract = {

Objective:\ To study and compare seasonal variation in camptothecin (CPT) content from bark samples of\ Nothapodytes nimmoniana\ obtained from geographically and climatologically isolated populations.\ Methods:\ A standard High Performance Liquid Chromatography methodology was used to analyze and quantify CPT from bark samples of\ N. nimmoniana.\ Results:\ Sample collected from Amboli yielded highest CPT content 1.337 g/100 g dry bark powder during the monsoon compared to other localities in study. Monsoon (August) showed to accumulate higher levels of CPT in barks of\ N. nimmoniana\ as compared to summer (May). Amboli averaged highest accumulation of CPT compared to other localities under study.\ Conclusion:\ These findings indicate season to have control over accumulation of CPT. Locality Amboli has highest CPT content in all seasons and were the elite population during the study. The study also suggests the need for further investigation in lights of biosynthesis in the plant.

}, keywords = {Camptothecin, High performance liquid chromatography, Nothapodytes Nimmoniana, Seasonal Variation}, doi = {10.4103/0974-8490.112434}, author = {Sandeep R Pai and Nilesh V Pawar and Mansingraj S Nimbalkar and Parthraj R Kshirsagar and Firdose K Kolar and Ghansham B Dixit} } @article {909, title = {Production of Camptothecin in Cultures of Chonemorpha grandiflora}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {November 2010}, pages = {296-299}, type = {Original Article}, chapter = {296}, abstract = {

Background: Chonemorpha grandiflora (Syn. Chonemorpha fragrans (Apocynaceae) is an endangered medicinal plant. It is used in different preparations, such as sudarsanasavam and kumaryasavam used in Kerala Ayurvedic system. C. grandiflora is used for the treatment of fever and stomach disorders. Phytochemical investigations have revealed the presence of steroidal alkaloids, such as chonemorphine and funtumafrine in C. grandiflora. Camptothecin, a well-known anticancer alkaloid has been detected in ethanolic extracts of stem with bark and callus cultures derived from C. grandiflora. Methods: Callus cultures of C. grandiflora were raised on Murashige and Skoog{\textquoteright}s medium supplemented with 2, 4-D. Stem with bark and callus were used for phytochemical analysis mainly the alkaloids. Detection and identification of camptothecin was carried out using thin-layer chromatography (TLC), high-performance thin-layer chromatography, (HPTLC) and high-performance liquid chromatography (HPLC). Results: An important anticancer alkaloid, camptothecin was detected in ethanolic extracts of stem with bark and callus cultures of C. grandiflora. camptothecin content was 0.013 mg/g in stem with bark and 0.003 mg/g in callus. Conclusion : This is the first report on in vivo and in vitro production of camptothecin in C. grandiflora. Camptothecin is known to occur only in six plant sources so, alternative sources for camptothecin are needed. Thus of C. grandiflora could be a new promising alternative source of camptothecin.

}, keywords = {Apocynaceae, Callus, Camptothecin, Chonemorpha grandiflora}, doi = {10.4103/0974-8490.72327}, author = {AV Kulkarni and AA Patwardhan and U Lele and NP Malpathak} }