@article {93, title = {Development and Validation of Veratric Acid in Tabebuia avellanedae using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry/Mass Spectrometry-Multiple Reaction Monitoring-Based Assay Coupled with 1,1-Diphenyl-2-Picrylhydrazyl Method}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {May 2020}, pages = {107-111}, type = {Original Article}, chapter = {107}, abstract = {

Objective: The aim of the present study is to develop a new simple, precise, and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of veratric acid in Tabebuia avellanedae Linn. Materials and Methods: The quantification of veratric acid was done using collision-induced dissociation multiple reaction monitoring scan of mass spectrometry technique. The developed method was validated according to the International Conference on Harmonization guidelines. The free radical scavenging activity was performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Results: The ion transitions of the precursor to the productions were principally protonated ion [M + H]+ at m/z 182.8 \>139.20, 124.20, and 77.0 for veratric acid. The proposed method was validated for linearity with an excellent correlation coefficient of 0.9978. The intraday and intermediate precisions and repeatability showed the percentage relative standard deviation was \<2\%. The accuracy for the determination of veratric acid was within 82.20\%{\textendash}97.65\%. The limit of detection and limit of quantitation were 0.66 and 2.21 ppm, respectively. The half-maximal inhibitory concentration value for the DPPH radical scavenging activity of ascorbic acid and quality control sample (hydroalcoholic extract of T. avellanedae) was found to be 17.79 and 36.44 μg/ml, respectively. Conclusion: The developed LC-MS/MS method is a simple, rapid, precise, accurate, and it is recommended for efficient assays in routine work. T. avellanedae exhibited strong DPPH radical scavenging activity.

}, keywords = {1, 1-diphenyl-2-picrylhydrazyl, Method validation, Multiple reaction monitoring, Tabebuia avellanedae, Veratric acid}, doi = {10.4103/pr.pr_77_19}, author = {Puttaswamy Naveen and Harakanahalli Basavegowda Lingaraju and Siddappa Chandrappa and Srikant Anitha and Kodimule Shyam Prasad} } @article {266, title = {Screening and Validating of 1, 1-Diphenyl-2-Trinitrophenylhydrazine Scavengers from Danshen-Honghua Herbal Pair}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {February,2019}, pages = {14-19}, type = {Original Article}, chapter = {14}, abstract = {

Background: Salvia miltiorrhiza (Danshen [DS]) and Carthamus tinctorius (Honghua [HH]) are commonly used traditional Chinese medicines for activating blood and removing stasis, and they were usually used as DS-HH (Danshen-Honghua [DH]) herbal pair in clinical applications. Characterizing the antioxidant active ingredients in DH herbal pair may be helpful for understanding their curative effect on cardiovascular diseases. Objective: The objective of the study is to screen the 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) scavenging active compounds in DH herbal pair by spectrum-effect relationship analysis. Materials and Methods: First, the water extracts of DH herbal pair with different ratios (DS: HH = 1:0, 0:1, 1:1, 2:1, 3:1, 5:1, 1:5, 1:3, and 1:2) were prepared. Then, the clearance effects of DH herbal pair and single drugs on DPPH were compared, meanwhile, high performance liquid chromatography was applied for chemical analysis of DH extracts. Finally, DPPH scavengers in DH herbal pair were predicted and identified by spectrum-effect relationship analysis and liquid chromatography-mass spectrometry (LC-MS) analysis. Results: Compared with single drugs, the extracts of herbal pairs had higher clearance rate to DPPH. Eighteen potential active compounds (peaks) in the extract of DH herbal pair were predicted, and 13 of them were tentatively identified by LC-MS analysis. Furthermore, antioxidant activities of eight pure compounds from the DH herbal pair were validated by DPPH radical scavenging assay with Vitamin C as positive control drug. Among them, six compounds including danshensu, protocatechuic acid, coffee acid, chlorogenic acid, rutin, and salvianolic acid A were found to have high antioxidant activity. Conclusion: DH herbal pair showed strong clearance effect on DPPH, and danshensu, protocatechuic acid, coffee acid, chlorogenic acid, rutin and salvianolic acid A are the active components.

}, keywords = {1, 1-diphenyl-2-trinitrophenylhydrazine scavenger, Active ingredients, Antioxidation effect, Danshen-Honghua, Spectrum-effect relationship analysis.}, doi = {10.4103/pr.pr_94_18}, author = {Ya-Li Wang, Shi-Jun Yin and Sheng-Li Huang, Qian Zhang and Hua Chen, Feng-Qing Yang} } @article {450, title = {Antioxidant Activity and Phenolic and Flavonoid Contents of the Extract and Subfractions of Euphorbia splendida Mobayen}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {November 2017}, pages = {362-365}, type = {Original Article}, chapter = {362}, abstract = {

Introduction: The harmful action of the free radicals which cause the oxidative stress can be blocked by antioxidant substances, and different plant extracts showed antioxidant activity. The aim of this study is was evaluation the antioxidant activity of total methanol extract (ME) and subfractions of Euphorbia splendida Mobayen. Materials and Methods: Aerial part of E. splendida was extracted by maceration with methanol and then subfractionated by liquid{\textendash}liquid fractionation using petroleum ether, chloroform, ethyl acetate, and water. Antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay, reduction of ferric ions and ferrous ion chelating potential. Total phenolic contents (TPC) and total flavonoid contents (TFC) were estimated with Folin-Ciocaltue and aluminum chloride methods, respectively. Results: The findings revealed that E. splendida ME and subfractions showed a dose-dependent antioxidant activity. ME showed the highest antioxidant activity based on total reduction capability and ferrous ions chelating assay tests. Aqueous fraction and then ethyl acetate fraction showed the best IC50 in DPPH radical scavenging test in comparison to butylated hydroxytoluene. ME showed the highest value of TPC and TFC (270.74 {\textpm} 0.005 mg/g and 208.23 {\textpm} 0.007 mg/g, respectively). Conclusion: This study showed that the extract and subfractions of E. splendida have antioxidant activity. The antioxidant activity of the extract and fractions might be attributed to the presence of phenolic compounds. More studies are needed to determine the active antioxidant compounds of this plant.

}, keywords = {1, 1-Diphenyl-1-picrylhydrazyl, Antioxidant, Euphorbia splendida, Flavonoid, Fractions, Free radicals}, doi = {10.4103/pr.pr_12_17}, author = {Zahra Kefayati and Saeed Mohammadi Motamed and Asie Shojaii and Mitra Noorix and Mitra Noori and Roshanak Ghods} } @article {383, title = {Investigation of Effect of 1,8-cineole on Antimicrobial Activity of Chlorhexidine Gluconate}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {July 2017}, pages = {234-237}, type = {Original Article}, chapter = {234}, abstract = {

Background: Chlorhexidine belongs to a group of medicines called antiseptic antibacterial agents. Chlorhexidine is commonly used for the care and clean off the skin, hands, and wounds. In recent years, medicinal and aromatic plants have been used for prevention of disease, maintaining health, and improving disease in traditional and modern medicine as a medicament. According to recent research, cineole is the isolated active agent of eucalyptus oil and possesses antimicrobial activity. It was demonstrated that cineole could enhance the antimicrobial effects of the other antiseptics. Objective: The aim of this study was to investigate the efficacy of 1,8-cineole on the antimicrobial effect of chlorhexidine against some microorganisms. Materials and Methods: The effect of 1,8-cineole on antimicrobial activity of chlorhexidine gluconate (CHG) was tested using seven different microorganisms. In this study, CHG (128{\textendash}0.125 mg/l) and cineole (512{\textendash}2 g/l) were analyzed together and separately using checkerboard assay. Interactions between CHG and 1,8-cineole have been identified as synergistic, indifferent, or antagonistic. Results: Synergistic activity was demonstrated between CHG and 1,8-cineole against Staphylococcus aureus, methicillin-resistant S. aureus, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, and Candida albicans. Indifferent interactions for these compounds were demonstrated against Pseudomonas aeruginosa. Conclusion: CHG antiseptic properties were found to be increased when CHG was used in combination with 1,8-cineole. In this way, CHG will reveal stronger effect against microorganisms.

}, keywords = {1, 8-cineole, Chequerboard assay, Chlorhexidine gluconate}, doi = {10.4103/0974-8490.210329}, author = {Merih Simsek and Re{\c s}at Duman} } @article {494, title = {Evaluation of Skin Anti-aging Potential of Citrus reticulata Blanco Peel}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {May 2016}, pages = {160-168}, type = {Original Article}, chapter = {160}, abstract = {

Background: The peel of Citrus reticulata Blanco is traditionally used as tonic, stomachic, astringent, and carminative. It is also useful in skin care. Objective: To study the anti-aging potential of alcoholic extracts of C. reticulata Blanco peel using in vitro antioxidant and anti-enzyme assays. Materials and Methods: Plant extracts were obtained by Soxhlation (CR HAE- Hot Alcoholic Extract of Citrus reticulata) and maceration method (CR CAE- Cold Alcoholic Extract of Citrus reticulata). Qualitative and quantitative phytochemical analysis was performed. Further, in vitro antioxidant, anti-enzyme, and gas chromatography-mass spectrometry (GC-MS) analyses were performed. Results: Total phenolic and flavonoid contents of CR HAE were found to be higher than CR CAE. EC50 value of CR HAE and CR CAE for 1,1-Diphenyl-2-picrylhydrazyl, Superoxide anion, and 2, 2{\textquoteright}-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assays were 250.33 {\textpm} 40.16 μg/ml and 254.73 {\textpm} 15.78 μg/ml, 221.27 {\textpm} 11.25 μg/ml and 354.20 {\textpm} 23.79 μg/ml, and 59.16 {\textpm} 2.17 μg/ml and 59.12 {\textpm} 6.21 μg/ml, respectively. Oxygen radical absorbance capacity values for CR HAE and CR CAE were found to be 1243 and 1063 μmoles 6-hydroxy-2,5,7,8-tetra methylchromane-2-carboxylic acid equivalent/g of substance, respectively. Anti-collagenase and anti-elastase activities were evaluated for both CR HAE and CR CAE. EC50 values of CR HAE and CR CAE for anti-collagenase and anti-elastase were 329.33 {\textpm} 6.38 μg/ml, 466.93 {\textpm} 8.04 μg/ml and 3.22 {\textpm} 0.24 mg/ml, 5.09 {\textpm} 0.30 mg/ml, respectively. CR HAE exhibited stronger anti-collagenase and anti-elastase activity than CR CAE. GC-MS analysis of CR HAE was carried out because CR HAE exhibited higher antioxidant and anti-enzyme potential than CR CAE. Conclusion: C. reticulata peel can be utilized in anti-wrinkle skin care formulations.

}, keywords = {1, 1-diphenyl-2-picrylhydrazyl, 2, 2{\textquoteright}-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), anti-wrinkle, elastase, Gas chromatography-mass spectrometry, oxygen radical absorbance capacity}, doi = {10.4103/0974-8490.182913}, author = {Vinita D. Apraj and Nancy S. Pandita} } @article {536, title = {Antiproliferative evaluation of terpenoids and terpenoid coumarins from Ferulago macrocarpa (Fenzl) Boiss. fruits}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {October 2015}, pages = {322-328}, type = {Original Article}, chapter = {322}, abstract = {

Background: Ferulago macrocarpa is a plant used as flavoring agent and protectant in the food industry and as a folk medicinal plant in Iran with no available information on its chemical identity. Ferulago spp. showed to contain biologically terpenoids and coumarins. Objectives: The objective was to isolate and characterize terpenoids and coumarins from the acetone extract of F. macrocarpa fruits and to evaluate their antiproliferative effects on several cell lines. Materials and Methods: A series of normal and reverse phase gravity and high-performance liquid chromatography analyses were used to purify constituents. Compounds 1{\textendash}5 and 7 were evaluated for their cytotoxic effects on MCF-7, HT-29 and H-1299 cell lines. Results: Six compounds including bornyl acetate (1), 1,10-di-epi-cubenol (2), stigmasterol (3) and three coumarins grandivittin (4), prantschimgin (5) and 4{\textquotedblright}-hydroxygrandivittin (7) along with mixtures of feruloyl derivatives (6a-6c) have been purified. Their structures were established by spectroscopic methods including nuclear magnetic resonance and MS analyses. Compound 2 showed moderate cytotoxicity effect with IC50 values of 5.0 and 6.7 μM on MCF-7 and HT-29, respectively. Conclusion: 1,10-di-epi-Cubenol could be considered as a potential proliferation inhibitor of MCF-7 and HT-29 cell lines.

}, keywords = {1, 10-di-epi-Cubenol, Bornyl acetate, Grandivittin, HT-29, MCF-7, Prantschimgin.}, doi = {10.4103/0974-8490.158437}, author = {Seyed-Ebrahim Sajjadi and Maryam Jamali and Yalda Shokoohinia and Gisya Abdi and Behzad Shahbazi and Ali Fattahi} } @article {427, title = {In vitro Antioxidant and Free Radical Scavenging Activity of Four Alkanna Species Growing in Iran}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {December 2014}, pages = {100-104}, type = {Original Article}, chapter = {100}, abstract = {

Background: Medicinal plants are recognized as sources of natural antioxidants that can protect biological system from oxidative stress. Objective: In this study, the antioxidant activities of four species of the Alkanna genus (Alkanna bracteosa, Alkanna frigida, Alkanna orientalis and Alkanna tricophila) were evaluated. Materials and Methods: The dried roots of plant samples (100 g) were extracted separately by percolation method with MeOH/H2O (80/20) and four fractions were provided respectively with hexane, chloroform, ethyl acetate, butanol and water. Antioxidant activities were investigated by using 1,1-diphenyl-2-picrylhydrazyl, ferric thiocyanate and thiobarbituric acid methods and compared with quercetin (as positive control). Results: The results indicated that the butanol fractions of four species had the highest antioxidant activity and radical scavenging activity compared with the other fractions studied (P \< 0.05). The 50\% effective concentration (half -\ effective doses) values of butanol fractions are less than quercetin and other fractions, so, these fractions showed potent antioxidant activity. This indicated that the active compounds in the root parts of Alkanna species dissolved in the butanol. All the fractions of four species of Alkanna had antioxidant activity, while, there were no significant differences (P \> 0.05) between the total antioxidant activities of same fractions. The total antioxidant activity values of Alkanna fractions in a descending order are as follows: Butanol fraction \> ethyl acetate fraction \> total extract \> hexane fraction \> chloroform fraction \> aqueous fraction. Conclusion: The antioxidant activities of butanol fractions of samples were higher than quercetin. This may be because most of the active compounds of Alkanna species dissolved in the butanol.

}, keywords = {1, 1-diphenyl-2-picrylhydrazyl, Alkanna, Antioxidant activity, Ferric thiocyanate, Thiobarbituric acid.}, doi = {10.4103/0974-8490.147218}, author = {Iraj Salimikia and Ali Reza Yazdinezhad and Fereshteh Golfakhrabadi and Hamid Reza Monsef Esfahani} } @article {744, title = {Comparative Study of Antidiabetic activity of Cajanus Cajan and Tamarindus indica in Alloxan-induced Diabetic Mice with a Reference to In vitro Antioxidant Activity}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {March,2014}, pages = {180-187}, type = {Original Article}, chapter = {180}, abstract = {

Background: Oxidative stress not only develops complications in diabetic (type 1 and type 2) but also contributes to beta cell destruction in type 2 diabetes in insulin resistance hyperglycemia. Glucose control plays an important role in the pro-oxidant/antioxidant balance. Some antidiabetic agents may by themselves have antioxidant properties independently of their role on glucose control. Objective: The present investigation draws a comparison of the protective antioxidant activity, total phenol content and the antihyperglycemic activity of the methanolic extract of Cajanus cajan root (MCC) and Tamarindus indica seeds (MTI). Materials and Methods: Antidiabetic potentials of the plant extracts were evaluated in alloxan-induced diabetic Swiss albino mice. The plant extracts at the doses of 200 and 400 mg/kg body weight was orally administered for glucose tolerance test during 1-hour study and hypoglycemic effect during 5-day study period in comparison with reference drug Metformin HCl (50 mg/kg). In vitro antioxidant potential of MCC and MTI was investigated by using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity at 517 nm. Total phenolic content, total antioxidant capacity and reducing power activity was also assayed. Results: There was a significant decrease in fasting serum glucose level (P \< 0.001), reduction in blood glucose level (P \< 0.001) in 5-days study, observed in the alloxan-induced diabetic mice. The reduction efficacy of blood glucose level of both the extracts is proportional to their dose but MCC is more potent than MTI. Antioxidant study and quantification of phenolic compound of both the extracts revealed that they have high antioxidant capacity. Conclusion: These studies showed that MCC and MTI have both hypoglycemic and antioxidant potential but MCC is more potent than MTI. The present study suggests that both MCC and MTI could be used in managing oxidative stress.

}, keywords = {1, 1- diphenyl-2-picrylhydrazyl, Alloxan, Antioxidant activity, Hyperglycemia, Metformin hydrochloride, Oxidative stress}, doi = {10.4103/0974-8490.129043}, author = {Laizuman Nahar and Fatema Nasrin and Ronok Zahan and Anamul Haque and Ekramul Haque and Ashik Mosaddik} } @article {725, title = {Development and Validation of a High Performance Thin Layer Chromatographic Method for Determination of 1, 8-Cineole in Callistemon Citrinus}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {March,2014}, pages = {143-147}, type = {Original Article}, chapter = {143}, abstract = {

A new, simple, precise, rapid, and selective high performance thin layer chromatographic (HPTLC) method has been developed and validated for the estimation of 1, 8-cineole in volatile oil of leaves of Callistemon citrinus obtained by hydro distillation. The method was validated as per ICH guidelines and can be utilized for routine analysis. The retention factor for 1, 8-cineole was found to be 0.52. The linearity was found to be in the range of 3 μg-12 μg. The recovery obtained for 1, 8-cineole was 98\%, which is satisfactory. The result obtained in validation indicate the accuracy, reproducibility, and reliability of the developed HPTLC method for determination of 1, 8-cineole.

}, keywords = {1, 8-cineole, Callistemon citrinus, Essential oil, High performance thin layer chromatographic}, doi = { 10.4103/0974-8490.129034}, author = {Archana Shaha and Vijay R Salunkhe} } @article {135, title = {Screening and Validating of 1, 1-Diphenyl-2-Trinitrophenylhydrazine Scavengers from Danshen-Honghua Herbal Pair}, journal = {Pharmacognosy Research}, volume = {11}, year = {2011}, month = {February 2019}, pages = {14-19}, type = {Original Article}, chapter = {14}, abstract = {

Background: Salvia miltiorrhiza (Danshen [DS]) and Carthamus tinctorius (Honghua [HH]) are commonly used traditional Chinese medicines for activating blood and removing stasis, and they were usually used as DS-HH (Danshen-Honghua [DH]) herbal pair in clinical applications. Characterizing the antioxidant active ingredients in DH herbal pair may be helpful for understanding their curative effect on cardiovascular diseases. Objective: The objective of the study is to screen the 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) scavenging active compounds in DH herbal pair by spectrum-effect relationship analysis. Materials and Methods: First, the water extracts of DH herbal pair with different ratios (DS: HH = 1:0, 0:1, 1:1, 2:1, 3:1, 5:1, 1:5, 1:3, and 1:2) were prepared. Then, the clearance effects of DH herbal pair and single drugs on DPPH were compared, meanwhile, high performance liquid chromatography was applied for chemical analysis of DH extracts. Finally, DPPH scavengers in DH herbal pair were predicted and identified by spectrum-effect relationship analysis and liquid chromatography-mass spectrometry (LC-MS) analysis. Results: Compared with single drugs, the extracts of herbal pairs had higher clearance rate to DPPH. Eighteen potential active compounds (peaks) in the extract of DH herbal pair were predicted, and 13 of them were tentatively identified by LC-MS analysis. Furthermore, antioxidant activities of eight pure compounds from the DH herbal pair were validated by DPPH radical scavenging assay with Vitamin C as positive control drug. Among them, six compounds including danshensu, protocatechuic acid, coffee acid, chlorogenic acid, rutin, and salvianolic acid A were found to have high antioxidant activity. Conclusion: DH herbal pair showed strong clearance effect on DPPH, and danshensu, protocatechuic acid, coffee acid, chlorogenic acid, rutin and salvianolic acid A are the active components.

}, keywords = {1, 1-diphenyl-2-trinitrophenylhydrazine scavenger, Active ingredients, Antioxidation effect, Danshen-Honghua, Spectrum-effect relationship analysis}, doi = {10.4103/pr.pr_94_18}, author = {Ya-Li Wang and Shi-Jun Yin and Sheng-Li Huang and Qian Zhang and Hua Chen and Feng-Qing Yang} } @article {830, title = {Determination of Polyphenols and Free Radical Scavenging Activity of Tephrosia purpurea linn Leaves (Leguminosae)}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {July 2010}, pages = {152-158}, type = {Original Article}, chapter = {152}, abstract = {

Background: Leaves of Tephrosia purpurea Linn. (sarpankh), belonging to the family Leguminaceae, are used for the treatment of jaundice and are also claimed to be effective in many other diseases. This research work was undertaken to investigate the in vitro antioxidant activity of aqueous and ethanolic extracts of the leaves. Method: The therapeutic effects of tannins and flavonoids can be largely attributed to their antioxidant properties. So, the quantitative determinations were undertaken. All the methods are based on UV-spectrophotometric determination. Result: The total phenolic content of aqueous and ethanolic extracts showed the content values of 9.44 {\textpm} 0.22\% w/w and 18.44 {\textpm} 0.13\% w/w, respectively, and total flavonoid estimation of aqueous and ethanolic extracts showed the content values of 0.91 {\textpm} 0.08\% w/w and 1.56 {\textpm} 0.12\%w/w, respectively, for quercetin and 1.85 {\textpm} 0.08\% w/w and 2.54 {\textpm} 0.12\% w/w, respectively, for rutin. Further investigations were carried out for in vitro antioxidant activity and radical scavenging activity by calculating its percentage inhibition by means of IC 50 values, all the extracts{\textquoteright} concentrations were adjusted to fall under the linearity range and here many reference standards like tannic acid, gallic acid, quercetin, ascorbic acid were taken for the method suitability. Conclusion: The results revealed that leaves of this plant have antioxidant potential. The results also show the ethanolic extract to be more potent than the aqueous decoction which is claimed traditionally. In conclusion, T. purpurea Linn. (Leguminosae) leaves possess the antioxidant substance which may be responsible for the treatment of jaundice and other oxidative stress-related diseases.

}, keywords = {1, 1-diphenyl-2-picrylhydrazyl-radical scavenging assay, Ferric reducing antioxidant power, Tephrosia purpurea Linn. (Leguminosae), Total phenolic content}, doi = {10.4103/0974-8490.65509}, author = {Avani Patel and Amit Patel and Amit Patel and NM Patel} } @article {787, title = {Chemical Investigation of Aerial Parts of Gmelina asiatica Linn by GC-MS}, journal = {Pharmacognosy Research}, volume = {1}, year = {2009}, month = {January 2010 }, pages = {152-156}, type = {Original Article}, chapter = {152}, abstract = {

This study was carried out to analyze the active constituents present in aerial parts of Gmelina asiatica Linn. (Verbenaceae). Twenty-two compounds in chloroform extract and 12 compounds in ethanolic extract were identified by Gas Chromatography {\textendash} Mass Spectrometry (GC-MS) analysis. 1, 2-Benzenedicarboxylic acid, diisooctyl ester (31.22 \%) was the prevailing compound in chloroform extract and Monolinoleoylglycerol trimethylsilyl ether (38.51\%) was the major constituent of ethanolic extract. This is the first report of identification of active constituents from aerial parts of Gmelina asiatica by GC-MS.

}, keywords = {1, 2-Benzenedicarboxylic acid diisooctyl ester, GC-MS, Gmelina asiatica (Verbenaceae), Monolinoleoylglycerol trimethylsilyl ether.}, doi = {Nil}, author = {N. J. Merlin and V. Parthasarathy and R. Manavalan and S. Kumaravel} }