@article {509, title = {Probing Regenerative Potential of Moringa oleifera Aqueous Extracts Using In vitro Cellular Assays}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {August 2016}, pages = {231-237}, type = {Original Article}, chapter = {231}, abstract = {

Background: Molecules stimulating regeneration and proliferation of cells are of significance in combating ailments caused due to tissue injury, inflammation, and degenerative disorders. Moringa oleifera is one of the most valued food plants having the profile of important nutrients and impressive range of medicinal uses. Objective: To evaluate the potential of M. oleifera aqueous leaf and flower extracts to promote the proliferation of cells and explore their effect on cancer cell lines for assessment of safety. Materials and Methods: Aqueous leaf and flower extracts of M. oleifera were investigated for effect on rat-derived primary fibroblast, mesenchymal stem cells (MSCs), and cancer cell lines using cell proliferation assay. They were also tested and compared for wound healing, angiogenesis, and hepatoprotective effect using in vitro assays. Results: Statistically significant increase in the proliferation of primary rat fibroblast, MSCs, and angiogenesis was observed after treatment with aqueous flower extract. The aqueous leaf extract determined a comparatively moderate increment in the proliferation of MSCs and angiogenesis. It however showed prominent cytotoxicity to cancer cell lines and a significant hepatoprotective effect. Conclusion: A very clear difference in response of the two extracts to different types of cells was detected in this study. The aqueous flower extract exhibited a higher potential to stimulate cell proliferation while not exerting the same effect on cancer cell lines. The leaf extract on the other hand, had a prominent antitumor and hepatoptotective effects.

}, keywords = {Aqueous, cytotoxicity, Fibroblast, in vitro, Viability}, doi = {10.4103/0974-8490.188877}, author = {Evangeline E. Fernandes and Anubha V. Pulwale and Gauri A. Patil and Alpana S. Moghe} } @article {841, title = {The Evaluation of Antinociceptive Activity of Alkaloid, Methanolic, and Aqueous extracts of Malaysian Mitragyna speciosa Korth leaves in rats}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {July 2010}, pages = {181-185}, type = {Original Article}, chapter = {181}, abstract = {

Mitragyna speciosa Korth is a medicinal plant indigenous to Thailand and Malaysia and has been known for its narcotic and coca-like effects. Many studies have been performed on the antinociceptive effect of the plant extracts of Thai origin; however, limited studies have been reported till date on M. speciosa extracts of Malaysian origin. Various concentrations of alkaloid (5{\textendash}20 mg/kg), methanolic (50{\textendash}200 mg/kg), and aqueous (100{\textendash}400 mg/kg) extracts of Malaysian M. speciosa leaves were prepared and orally administered to nine groups of rats. Morphine (5 mg/kg, s.c.) and aspirin (300 mg/kg, p.o.) were used as control. Antagonism of the antinociceptive activity was evaluated by pretreatment with naloxone at a dose of 2 mg/kg (i.p.). Results showed that oral administration of the alkaloid (20 mg/kg), methanolic (200 mg/kg), and aqueous (400 mg/kg) extracts significantly prolonged the latency of nociceptive response compared with control groups in both hot plate and tail flick tests (P \< 0.05). Antinociceptive action of the alkaloid (20 mg/kg), methanolic (200 mg/ kg), and aqueous (400 mg/kg) extracts was significantly blocked by naloxone. In conclusion, these results suggest the presence of antinociceptive effect in various extracts of Malaysian M. speciosa leaves. In addition, the antinociceptive effective doses vary depending on the type of solvents used for extraction.

}, keywords = {Alkaloid, Antinociceptive, Aqueous, Extract, Methanol, Mitragyna speciosa}, doi = {10.4103/0974-8490.65514}, author = {Azadeh Sabetghadam and Surash Ramanathan and Sharif Mahsufi Mansor} }