@article {1014, title = {GC-MS Analysis and Thrombolytic Property of Methanolic Leaf Extracts of Terminalia pallida Brandis against Carrageenan Instigated Tail Thrombosis Model in Mice}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {January 2021}, pages = {53-60}, type = {Research Article}, chapter = {53}, abstract = {

Background: Several plants of Terminalia species are being reported as medicinally useful. Terminalia pallida Brandis is one of the plants of the Combretaceae family, which constitutes several pharmacologically active substances. Present work explored leaf extract{\textquoteright}s thrombolytic property, which could serve as the superior choice for currently using drugs. Objectives: The present study primarily focused on investigating the phyto-constituents such as phenolic and flavonoid compounds in methanolic leaf extract followed by GC-MS analysis and determining the in vitro thrombin inhibition and in vivo thrombolysis activity. Materials and Methods: Swiss albino mice (total = 36) were randomly distributed into six groups (n=6). Thrombosis was instigated by injecting 40 μL of 1\% carrageenan (Type-I) via the subplantar region of the right hind paw of each mouse. The plant extract was screened for its in vitro thrombin inhibitory potency. In animal studies, the size of the blood clots in mouse tails was recorded by administering the plant extracts at 100, 200 and 300 mg/kg body weight for every 24, 48 and 72 hr, respectively. Results: A dose of 200 and 300 mg/kg of the extract showed significant thrombolytic activity at 24, 48, and 72 hr (p\< 0.001) in a concentration-dependent manner when correlated with the control group. A reduction in the length of blood clots (p\< 0.01) was observed at 48 and 72 hr. In acute oral toxicity study, administration of leaf extract showed no mortality and no significant behavioral changes up to 2000 mg/kg dose. The GC-MS analysis explored the occurrence of about 16 eluted compounds; among these, few have been reported as medicinally useful, which accounts for the therapeutic importance of the plant. Conclusion: In conclusion, the methanolic extract of T. pallida Brandis leaves at 200, and 300 mg/kg showed significant inhibition of the Thrombosis in carrageenan instigated model of mice and in vitro thrombin activity.

}, keywords = {Carrageenan, Heparin, Quercetin., Terminallia pallida Brandis, Thrombosis}, doi = {10.5530/pres.14.1.9}, url = {https://phcogres.com/fulltext/v14i1/105530pres1419.html}, author = {Sarvan Kumar Guguloth and Narender Malothu and Prasanth DSNBK} } @article {433, title = {Oral Intake of Polyphenols of Chromolaena odorata: A Perspective in Peptic Ulcer, Thrombocytopenia, and Heparin-induced Bleeding Diathesis in Rodent Model}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {October 2018}, pages = {426-431}, type = {Original Article}, chapter = {426}, abstract = {

Objective: Bleeding disorder is of two types: external bleeding due to trauma, surgery, and internal bleeding which happens mainly due to ulcers and thrombocytopenia or use of excessive anticoagulants. Proven hemostatic activity of Chromolaena odorata (L) in bleeding and wound healing had encouraged to evaluate the efficacy of aqueous extract and spray-dried powder (SDP) on ulcers, thrombocytopenia, and heparin-induced bleeding diathesis. Materials and Methods: Internal bleeding in the form of lesions in empty stomach was induced by absolute ethanol. Busulfan is administered through intraperitoneal injection to produce thrombocytopenia in Wistar rats and heparin at 1000 IU/ml was injected intravenous to mice, and after 30 min, tail was truncated to qualitatively and quantitatively differentiate the bleeding diathesis in treated versus nontreated controls. Results: A significant reduction in the lesions was observed in treated rats as compared to disease control rats which was evident from ulcer index and histopathology data. Considerable protection and production of platelets were observed in the thrombocytopenia model which was drastically reduced in the Busulfan administered disease control. Both aqueous extract and SDP were successful in preventing blood loss in heparin-induced tail-bleeding model. Conclusion: The presence of polyphenols attributes to the crucial role of C. odorata in arresting internal bleeding from stomach ulcer, protecting the thrombocytes from destroying, and minimizing the bleeding diathesis in mice.

}, keywords = {Chromolaena odorata, Gastric ulcer, Heparin, Tail-bleeding assay, Thrombocytopenia}, doi = {10.4103/pr.pr_107_18}, author = {Tania S. Paul and Biswadeep B. Das and Shakti P. Ingale and Niruta Killedar and Kishori G. Apte} }