@article {423, title = {Seabuckthorn (Hippophae rhamnoides and Hippophae salicifolia) Seed Oil in Combating Inflammation: A Mechanistic Approach}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {October 2018}, pages = {404-407}, type = {Original Article}, chapter = {404}, abstract = {

Objective: This study assessed and compared in vivo anti-inflammatory activity of Hippophae rhamnoides (HR) and Hippophae salicifolia (HS) seed oil. Materials and Methods: HR and HS seed oil was extracted by Soxhlet apparatus and characterized using gas chromatography mass spectroscopy. Wistar rats were used for predicting anti-inflammatory activity. Results: HR and HS (2 and 4 ml/kg, respectively) exhibited dose-dependent inhibition of carrageenan-, histamine-, prostaglandin-, bradykinin-, and arachidonic acid (AA)-induced paw edema. Significant leukotriene-induced inhibition was observed in HR. Myeloperoxidase (MPO) and lipid-peroxidase (LPO) assays were performed and HR and HS seed oil significantly decreased the level of MPO and LPO at 4 ml/kg dose (P \< 0.001). Conclusion: Dual inhibition of AA metabolism in HR and cyclooxygenase inhibition in HS was observed that might be attributed to the presence of polyunsaturated fatty acids (PUFAs), specifically, a correct balance of n-3 and n-6 PUFAs. However, the findings should be interpreted in the light of limitation of this study. Detailed experimentation at enzymatic levels would further help in substantiating the results inferred in this study.

}, keywords = {Antibradykinin, Antihistaminic, Arachidonic acid, Leukotriene, Polyunsaturated fatty acid, Prostaglandin}, doi = {10.4103/pr.pr_71_18}, author = {Suchita Dubey and M. V. Ramana and Anuradha Mishra and Pushpraj S. Gupta and Himani Awasthi} }