@article {1294, title = {Rosa indica Leaf Contains Molecules with Promising Antioxidant, Antimicrobial and Anticancer Effects in vitro with Strong Interaction with Human VEGFR2 in silico}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {793-802}, type = {Original Article}, chapter = {793}, abstract = {

Background: Plants-based compounds/biopharmaceuticals are found to be effective against multiple ailments due to their fewer side effects. Objectives: In this study, the antioxidant, antibacterial and cytotoxic properties and potential secondary metabolites of Rosa indica were evaluated. Materials and Methods: The extract of R. indica leaf was prepared and tested for in vitro Antioxidant and anti-cancer potential. Using HR LCMS QTOF analysis, a secondary metabolite fingerprint has been identified. The ADME properties of prospective molecules, as well as their molecular docking and molecular dynamics with VEGFR2, were investigated in silico. Results: The result showed that the extract had profound antioxidant activity and showed cytotoxicity against breast cancer cells (MDA-MB) and also showed significant antimicrobial activity against several human pathogens, especially S.aureus and K. pneumoniae. HR LCMS analysis revealed several secondary metabolites with significant bioactive potential. Among the several compounds identified, a few compounds showed strong ADME properties. Docking studies showed that Maritimetin and Santin had a stronger binding affinity for human VEGFR2 than the standard inhibitor. Santin, maritimetin and vitexin, subjected to MD simulations showed an RMSD value, which consistently remained within 4 {\r A} for both protein and ligands reflecting the stability during drug interaction. Conclusion: According to the current research, R. indica has several bioactive substances that may be developed into medicinal agents.

}, keywords = {Antibacterial, HR-LCMS, In vitro antioxidant, MDA-MB cells, Rosa indica}, doi = {10.5530/pres.16.4.91}, author = {Sini Hariharan and Neema Job and Kottayath Govindan Nevin and Arun Kumar Gangadharan} } @article {398, title = {In vitro Antidiabetic Activity of Polar and Nonpolar Solvent Extracts from Leucas aspera (Willd.) Link Leaves}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {July 2017}, pages = {261-265}, type = {Original Article}, chapter = {261}, abstract = {

Background: Diabetes mellitus is a chronic illness, and the management of diabetes is a global problem. Successful treatment is required to prevent complications and organ damages. Herbal medicines are having minimal adverse effects when compared to the available synthetic drugs to treat such chronic diseases and disorders. Objective: The present study was aimed to evaluate the antidiabetic and antioxidant activity of polar and nonpolar solvent extracts of Leucas aspera (Willd.) link leaves under in vitro models. Materials and Methods: The in vitro antidiabetic activity of petroleum ether (nonpolar) and ethanol (polar) extracts were evaluated in C2C12 cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (cell viability method) and glucose uptake assay. 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method used for the evaluation of in vitro antioxidant activity. Results: Both the polar and nonpolar solvent extracts of L. aspera had shown better antioxidant activity compared to standard (IC50 = 18.96 and 19.90 μg/mL, respectively). Petroleum ether extract exhibited better cytotoxic activity in C2C12 cell line compared to ethanol extract (concentration of test drug needed to inhibit cell growth by 50\% 110.75 {\textpm} 5.5 vs. 415.25 {\textpm} 8.0 μg/mL) whereas ethanol extract showed enhanced glucose uptake activity than petroleum ether extract in C2C12 cell line at same concentrations. Conclusion: From our study results, we concluded that L. aspera (Willd.) link leaves had shown better antidiabetic activity and antioxidant activity under in vitro models. Nonpolar solvent extract produced slightly better activity than polar solvent extract. This study warrants further research and experiments on animal models.

}, keywords = {In vitro antidiabetic, In vitro antioxidant, Leucas aspera, Nonpolar solvent, Polar solvent}, doi = {10.4103/pr.pr_141_16}, author = {VM Annapandian and R Shanmuga Sundaram} }