@article {1093, title = {Quantitative Estimation of Immunomodulatory Flavonoid Quercetin by HPTLC in Different Leafy Vegetables Available in West Bengal}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {October 2022}, pages = {423-428}, type = {Original Article}, chapter = {423}, abstract = {

Introduction: Leafy vegetables are commonly consumed medicinal plants used in various metabolic and infectious diseases. Additionally, their antiviral, antioxidant, anticancer, and antihypertensive activities have been reported in the literature. Objectives: To determine the quantity of immunomodulatory flavonoid, quercetin in different types of leafy vegetables available in West Bengal by using a simple validated HPTLC method. Materials and Methods: The chromatographic analysis was performed by using aluminium-backed silica gel 60 F254 plates with Toluene{\textendash}Ethyl acetate{\textendash}Formic acid 5:4:0.2 (\%v/v) as the mobile phase. The method was validated according to the ICH guidelines. Results: The total flavonoid content (TFC) in the four leafy vegetables studied varied between 58.10 to 12.28 mg QE/g. Well-separated and compact spots (Rf) of quercetin (0.55{\textpm}0.03) were detected. The regression equation obtained was y = 0.0011x - 0.0569, with a correlation coefficient (R{\texttwosuperior}) of 0.9939. The linearity range (μg/spot) was 60-160. The LOD/LOQ (ng/spot) was 6.09/18.47. Murraya koenigii (0.1992{\textpm}0.037 \%w/w) contained the maximum amount of quercetin compared to Ipomoea aquatic (0.1501{\textpm}0.039\%w/w), Coriandrum sativum (0.1430{\textpm}0.061\%w/w) and Trigonella foenum- graecum (0.1201{\textpm}0.055\%w/w) in methanolic extract. Conclusion: This study revealed that the validated HPTLC method was simple, accurate and sensitive for separating and quantifying quercetin in different leaf vegetables. Quercetin content was highest in Murraya koenigii (0.1992{\textpm}0.037 \%w/w), and least in Trigonella foenum-graecum (0.1201{\textpm}0.055 \%w/w). The developed method might be used further in standardizing and quality control of secondary metabolites in herbal formulations

}, keywords = {Flavonoid, HPTLC, Leafy vegetables, Quercetin}, doi = {10.5530/pres.14.4.62}, author = {Tushar Adhikari and Prerona Saha} } @article {407, title = {Development of Validated High-performance Thin-layer Chromatography Method for Simultaneous Determination of Quercetin and Kaempferol in Thespesia populnea}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {July 2017}, pages = {277-281}, type = {Original Article}, chapter = {277}, abstract = {

Introduction: Thespesia populnea L. (Family: Malvaceae) is a well-known medicinal plant distributed in tropical regions of the world and cultivated in South Gujarat and indicated to be useful in cutaneous affections, psoriasis, ringworm, and eczema. Bark and fruits are indicated in the diseases of skin, urethritis, and gonorrhea. The juice of fruits is employed in treating certain hepatic diseases. The plant is reported to contain flavonoids, quercetin, kaempferol, gossypetin, Kaempferol-3-monoglucoside, β-sitosterol, kaempferol-7-glucoside, and gossypol. T. populnea is a common component of many herbal and Ayurvedic formulation such as Kamilari and Liv-52. Objective: The present study aimed at developing validated and reliable high-performance thin layer chromatography (HPTLC) method for the analysis of quercetin and kaempferol simultaneously in T. populnea. Method: The method employed thin-layer chromatography aluminum sheets precoated with silica gel as the stationary phase and toluene: ethyl acetate: formic acid (6:4:0.3 v/v/v) as the mobile phase, which gave compact bands of quercetin and kaempferol. Result: Linear regression data for the calibration curves of standard quercetin and kaempferol showed a good linear relationship over a concentration range of 100{\textendash}600 ng/spot and 500{\textendash}3000 ng/spot with respect to the area and correlation coefficient (R2) was 0.9955 and 0.9967. The method was evaluated regarding accuracy, precision, selectivity, and robustness. Limits of detection and quantitation were recorded as 32.06 and 85.33 ng/spot and 74.055 and 243.72 ng/spot for quercetin and kaempferol, respectively. Conclusion: We concluded that this method employing HPTLC in the quantitative determination of quercetin and kaempferol is efficient, simple, accurate, and validated.

}, keywords = {High-performance thin-layer chromatography, Kaempferol, Quercetin, Thespesia populnea}, doi = {10.4103/0974-8490.210326}, author = {Hiteksha Panchal and Aeshna Amin and Mamta Shah} } @article {518, title = {Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {August 2016}, pages = {281-286}, type = {Original Article}, chapter = {281}, abstract = {

Background: Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. Objective: Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species. Materials and Methods: Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS. Results: Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments. Conclusions: Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.

}, keywords = {Benincasa hispida, Carissa congesta, Liquid chromatography, Mass spectroscopy, Polyalthia longifolia, Quercetin, Rutin}, doi = {10.4103/0974-8490.186578}, author = {Gaurav M. Doshi and Vivek V. Nalawade and Aaditi S. Mukadam and Pratip K. Chaskar and Sandeep P. Zine and Rakesh R. Somani and Hemant D. Une} } @article {508, title = {Evaluation of Antiulcer and Antioxidant Activity of Barleria gibsoni Dalz. Leaves}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {August 2016}, pages = {226-230}, type = {Original Article}, chapter = {226}, abstract = {

Background: Peptic ulcer is a digestive disorder most commonly found in clinical practice. Given the many side effects of modern medicine, the initial acquisition of fewer side effects, and medication of indigenous drugs, it should be considered as a better alternative for the treatment of peptic ulcer. Objective: To assess antiulcer and antioxidant activity of ethanol extract of Barleria gibsoni (EBG) Dalz. leaves in ulcer-induced rats and in vitro antioxidants method, respectively. Materials and Methods: Ethanol EBG was screened for antiulcer activity in pylorus ligation-induced ulcer models in Wistar rats. In vitro antioxidant activity of the extracts was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) radical scavenging activity. Total phenol and flavonoid content in the extracts were determined spectrophotometrically. Results: Oral administration of ethanol extract of leaves at doses of 250, 500 mg/kg p.o. reduced significant gastric lesions induced by pylorus ligation-induced ulcer as compared to standard omeprazole (20 mg/kg p.o.). The IC50 values were found to be 150 μg/mL in leaves extract. The ethanol extracts showed good antioxidant capacity in DPPH radical scavenging assay and NO radical scavenging activity when compared to standard. The total phenolic content using Folin{\textendash}Ciocalteu reagent estimated in 1 mg of leaves extracts was 368 μg and 481 μg with gallic acid equivalent and also the total flavonoid content found to be 240 and 410 μg, respectively, with quercetin equivalence. Conclusion: These findings suggest that the leaves of B. gibsoni possessed antiulcer potential and antioxidant compared to standard. This is the first ever report of antiulcer and antioxidant activities in B. gibsoni (Acanthaceae).

}, keywords = {Acanthaceae, Barleria gibsoni, Gallic acid, L-ascorbic acid, Pylorus ligation-induced ulcer model, Quercetin}, doi = {10.4103/0974-8490.188879}, author = {Firoj A. Tamboli and Harinath N. More} } @article {385, title = {Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {December 2015}, pages = {37-42}, type = {Original Article}, chapter = {37}, abstract = {

Objective: In Indian Ayurvedic system, Benincasa hispida (BH) and Carissa congesta (CC) are well-known plants used for major and minor ailments. BH has been regarded as Kushmanda, whereas CC has been used in immune-related disorders of the human system. Quercetin and rutin identified from the vast plethora of plant extracts have proved to possess ethnopharmacological relevance. Materials and Methods: In present studies, we have determined quercetin and rutin in terms of percentage in BH seeds and CC roots by high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC). After extraction and phytochemical screening, the extracts were subjected to quantification for the presence of quercetin and rutin by HPTLC and HPLC. Results: HPTLC showed quercetin as 44.60, 27.13\% and rutin as 32.00, 36.31\% w/w, whereas HPLC revealed quercetin as 34.00, 35.00\% and rutin as 21.99, 45.03\% w/v in BH and CC extracts, respectively. Conclusion: The BH and CC extracts have elucidated peaks that were corresponding with standard peaks on undertaking chromatographic studies.

}, keywords = {Benincasa hispida, Carissa congesta, High-performance liquid chromatography, high-performance thin layer chromatography, Quercetin, Rutin}, doi = {10.4103/0974-8490.171098}, author = {Gaurav Mahesh Doshi and Hemant Devidas Une} } @article {426, title = {Psidium guajava Linn. Leaf Extract Affects Hepatic Glucose Transporter-2 to Attenuate Early Onset of Insulin Resistance Consequent to High Fructose intake: An Experimental Study}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June 2015}, pages = {166-175}, type = {Original Article }, chapter = {166}, abstract = {

Background: Insulin resistance (IR) is amalgam of pathologies like altered glucos metabolism, dyslipidemia, impaired glucose tolerance, non-alcoholic fatty liver disease, and associated with type-II diabetes and cardiometabolic diseases. One of the reasons leading to its increased and early incidence is understood to be a high intake of processed fructose containing foods and beverages by individuals, especially, during critical developmental years. Objective: To investigate the preventive potential of aqueous extract of Psidium guajava leaves (PG) against metabolic pathologies, vis-{\`a}-vis, IR, dyslipidemia, hyperleptinemia and hypertension, due to excess fructose intake initiated during developmental years. Materials and Methods: Post-weaning (4 weeks old) male rats were provided fructose (15\%) as drinking solution, ad libitum, for 8 weeks and assessed for food and water/fructose intake, body weight, fasting blood sugar, mean arterial pressure, lipid biochemistry, endocrinal (insulin, leptin), histopathological (fatty liver) and immunohistochemical (hepatic glucose transporter [GLUT2]) parameters. Parallel treatment groups were administered PG in doses of 250 and 500 mg/kg/d, po {\texttimes} 8 weeks and assessed for same parameters. Using extensive liquid chromatography-mass spectrometry protocols, PG was analyzed for the presence of phytoconstituents like Myrecetin, Luteolin, Kaempferol and Guavanoic acid and validated to contain Quercetin up to 9.9\%w/w. Results: High fructose intake raised circulating levels of insulin and leptin and hepatic GLUT2 expression to promote IR, dyslipidemia, and hypertension that were favorably re-set with PG. Although PG is known for its beneficial role in diabetes mellitus, for the first time we report its potential in the management of lifelong pathologies arising from high fructose intake initiated during developmental years.

}, keywords = {Fructose, Glucose Ttransporter-2, Insulin resistance, Leptin, Psidium guajava, Quercetin}, doi = {10.4103/0974-8490.151459 }, author = {R. Mathur and Shagun Dutta and T. Velpandian and S.R. Mathur} } @article {731, title = {Pioglitazone, Quercetin and Hydroxy Citric Acid Effect on Hepatic Biomarkers in Non Alcoholic Steatohepatitis}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {March,2014}, pages = {153-162}, type = {Original Article}, chapter = {153}, abstract = {

Background: Non alcoholic steatohepatitis (NASH), severe form of diseases belonging to the spectrum of the Non alcoholic fatty liver disease (NAFLD). It is an asymptomatic disease which leads to fibrosis and finally to cirrhosis, an end stage liver disease. Objective: To study the effect of pioglitazone, quercetin and hydroxy citric acid on hepatic biomarkers and various biochemical parameters in experimentally induced non alcoholic steatohepatitis (NASH). Materials and Methods: Male Wister rats were divided into 8 groups. The activities of alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH) and γ-Glutamyl Transferase (GGT) were assayed in serum. The levels of various other biochemical parameters such as serum albumin, total bilirubin, creatinine, urea, uric acid and glucose were also estimated in experimental NASH. Results: The NASH group produced severe liver injury by significantly increasing the serum levels of ALT, AST, GGT and LDH compared with that of the control. However, the experimental NASH rats treated with pioglitazone, with quercetin and with hydroxy citric acid showed an obvious decrease in ALT, AST, GGT and LDH levels when compared with that of NASH induced group. A significant increase in the levels of albumin, creatinine, urea, uric acid, glucose and total bilirubin was noticed in experimentally induced NASH group (group 2) when compared to rats in control group (group 1). Conclusion: It could be inferred from this study that, pioglitazone, quercetin and hydroxy citric acid may afford protection to the liver against NASH, as evidenced by the results of this study on the levels of various biochemical parameters such as glucose, urea, uric acid, creatinine and bilirubin. Whereas from the results of hepatic marker enzymes, it is evident that optimal protection was observed after quercetin treatment against experimental NASH whereas pioglitazone and hydroxy citric acid also confers protection to some extent against NASH.

}, keywords = {Biochemical parameters, Experimentally induced NASH, hydroxy citric acid, Liver marker enzymes, Non alcoholic fatty liver disease, Non alcoholic steatohepatitis, pioglitazone, Quercetin}, doi = {10.4103/0974-8490.129037}, author = {Krishna Mohan Surapaneni and Mallika Jainu} } @article {749, title = {Colon-targeted quercetin delivery using natural polymer to enhance its bioavailability}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, month = {April 2011}, pages = {35-39}, type = {Original Article}, chapter = {35}, abstract = {

The aim of the present study is to develop a polymer (Guar Gum)-based matrix tablet (using quercetin as a model drug) with sufficient mechanical strength, and promising\ in vitro\ mouth-to-colon release profile. By definition, an oral colonic delivery system should retard drug release in the stomach and small intestine, and allow complete release in the colon. By drug delivery to the colon would therefore ensure direct treatment at the disease site, lower dosing, and fewer systemic side effects. Quercetin is antioxidant in nature and used to treat colon cancer, but they have poor absorption in the upper part of the gastrointestinal tract (GIT). As a site for drug delivery, the colon offers a near neutral pH, reduced digestive enzymatic activity, a long transit time, and an increased responsiveness to absorption enhancers. By achieving a colon-targeted drug delivery system, the absorption of quercetin may be increased, which leads to better bioactivity in fewer doses.

}, keywords = {Antioxidant, Colon cancer, Guar gum, Quercetin}, doi = {10.4103/0974-8490.79113}, author = {Anil Singhal and Jain H and Vipin Singhal and Edwin J. Elias and Ahmad Showkat} }