@article {1272, title = {Assessing the Antiurolithiatic Potentials of Calamus floribundus Griff. An in vitro and in vivo Approaches}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {June 2024}, pages = {604-615}, type = {Original Article}, chapter = {604}, abstract = {

Background: Urolithiasis, which is acquiring clinical relevance, is one of the most prevalent diseases seen in urology clinics. The most often reported uroliths in humans are oxalate, struvite, urate, brushite and cystine. The globally rising occurrence and severity of urolithiasis has made it an issue of demanding medical attention. Medicinal herbs and natural substances have been shown to be useful in the treatment and management of urolithiasis across various recent investigations. Calamus floribundus Griff. belongs to the Aracaceae family and its urolithiatic potential has yet to be established. Objectives: The objective of the study is to evaluate the antiurolithiatic potential of methanolic extract Calamus floribundus Griff. utilizing in vitro and in vivo methods. Materials and Methods: Nucleation assay and aggregation assay was done to evaluate the in vitro antiurolithiatic activity. In the in vivo studies ethylene glycol induced and glycolic acid induced model were used. MTT assay was done to evaluate the cytotoxic effect of the methanolic extract of Calamus floribundus Griff. Results: The IC50 value of the extract was found to be 767.394{\textpm}0.22 μg/mL and 759.435{\textpm}0.29 μg/mL in the nucleation and aggregation assay respectively. In the in vivo study all the biochemical parameters were normalised in dose dependent manner. Methanolic extract exhibited cytotoxic effect on NRK-52E cell lines with a IC50 value of 11.1 μg/mL. Conclusion: The obtained results indicates for the antiurolithiatic potential of Calamus floribundus Griff.

}, keywords = {Calamus floribundus Griff, Kidney stone, Nephrolithiasis, Urolithiasis}, doi = {10.5530/pres.16.3.72}, author = {Kangkan Deka and Bibhuti Bhusan Kakoti and Ngurzampuii Sailo and Zonunmawii and Dev Jyoti Kalita and Rajashri Bezbaruah} } @article {363, title = {Solubility of Calcium Oxalate and Calcium Phosphate Crystallization in the Presence of Crude Extract and Fractions from Kelussia odoratissima Mozaff}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {October 2018}, pages = {379-384}, type = {Original Article}, chapter = {379}, abstract = {

Background and Aim: Urinary tract stones are the third common urinary tract disease that may lead to renal failure. Kelussia odoratissima is traditionally used in the treatment of kidney stones in Chaharmahal va Bakhtiari region. This study was designed to investigate in vitro effect of crude extract and fractions of K. odoratissima on kidney stones (calcium oxalate and calcium phosphate). Materials and Methods: A total of 70\% ethanolic extract of K. odoratissima was prepared by maceration method followed by liquid{\textendash}liquid extraction with hexane, chloroform, ethyl acetate, and saturated n-butanol to get four fractions. Calcium oxalate and calcium phosphate were synthesized and then were treated with hydroalcoholic extract and fractions. Their effects on the dissolution of generated stone were assayed by calcium kit. The stones were synthesized and confirmed by fourier-transform infrared technique. Results: Results showed that total extract and its fractions had significant potency to dissolve calcium oxalate and calcium phosphate crystals. The results indicate the higher potency of fractions containing nonpolar compounds to dissolve calcium phosphate and calcium oxalate stones compared to the fractions containing polar compounds. n-butanolic fraction had the least effect and hexane fraction had the greatest effect on the calcium phosphate stones. Furthermore, the total extract has less dissolution ability, compared to the fractions. Conclusion: The obtained results of this study exhibited that the use of K. odoratissima extract and its fractions could help to dissolve urinary stones. Therefore, it can be effective in prevention and treatment of kidney stones on people who are prone to the formation of calcium oxalate and phosphate stones.

}, keywords = {Calcium oxalate, Calcium phosphate, Extract, Kelussia odoratissima, Kidney stone}, doi = {10.4103/pr.pr_68_18}, author = {Akram Torki and Tahereh Hosseinabadi and Sheida Fasihzadeh and Arezo Sadeghimanesh and Joko Priyanto Wibowo and Zahra Lorigooini} }