@article {295, title = {Anti-inflammatory Effect of Procumbenoside B from Justicia spicigera on Lipopolysaccharide-Stimulated RAW 264.7 Macrophages and Zebrafish Model}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {April 2018}, pages = {218-224}, type = {Original Article}, chapter = {218}, abstract = {

Background: Justicia spicigera is widely used in the Mexican traditional medicine for the treatment of inflammation. Objectives: The purpose of this study was to investigate the anti-inflammatory effect of procumbenoside B (PB) from J. spicigera on pro-inflammatory mediators. Materials and Methods: Lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and Zebrafish model were used to assess the potential anti-inflammatory effects of PB; its structure was elucidated on the basis of spectroscopic data. The production of inflammatory mediators such as interferon-β, prostaglandin E2, inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-κB) p65, interleukin-6 (IL-6), IL-1β, IL-12, cyclooxygenase (COX-2), tumor necrosis factor-α, and anti-inflammatory IL-10 was measured using enzyme-linked immunosorbent assay. NO production was measured using Griess reagent. Results: In LPS-induced-inflammatory response in RAW264.7 macrophage cells, PB strongly inhibited secretion of all pro-inflammatory mediators test and increased the production of IL-10 and blockade of NF-κB. In addition, PB suppressed LPS-stimulated nitric oxide and reactive oxygen species generation in a zebrafish model. Conclusion: These results indicated that the anti-inflammatory effects of PB may be attributed to the downregulation of iNOS and COX-2 through the suppression of NF-κB signaling pathway in RAW264.7 macrophages.

}, keywords = {Anti-inflammatory, Cytokines, Justicia spicigera, Macrophage model, Procumbenoside B, Zebrafish model}, doi = {10.4103/pr.pr_97_17}, author = {Rosa Martha Perez Gutierrez and Jose Maria Mota Flores and Adriana Maria Neira Gonzalez} }