@article {263, title = {Analysis of Soluble Proteins in Natural Cordyceps sinensis from Different Producing Areas by Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis and Two-dimensional Electrophoresis}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {February 2017}, pages = {34-38}, type = {Original Article}, chapter = {34}, abstract = {

Background: As one of the bioactive components in Cordyceps sinensis (CS), proteins were rarely used as index components to study the correlation between the protein components and producing areas of natural CS. Objective: Protein components of 26 natural CS samples produced in Qinghai, Tibet, and Sichuan provinces were analyzed and compared to investigate the relationship among 26 different producing areas. Materials and Methods: Proteins from 26 different producing areas were extracted by Tris-HCl buffer with Triton X-100, and separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). Results: The SDS-PAGE results indicated that the number of protein bands and optical density curves of proteins in 26 CS samples was a bit different. However, the 2-DE results showed that the numbers and abundance of protein spots in protein profiles of 26 samples were obviously different and showed certain association with producing areas. Conclusions: Based on the expression values of matched protein spots, 26 batches of CS samples can be divided into two main categories (Tibet and Qinghai) by hierarchical cluster analysis.

}, keywords = {Hierarchical cluster analysis, Natural Cordyceps sinensis, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Soluble protein, Two-dimensional electrophoresis}, doi = {10.4103/0974-8490.199782}, author = {Chun-Hong Li and Hua-Li Zuo and Qian Zhang and Feng-Qin Wang and Yuan-Jia Hu and Zheng-Ming Qian and Wen-Jia Li and Zhi-Ning Xia and Feng-Qing Yang} } @article {438, title = {Identification of the Adulterated Asini Corii Colla with Cytochrome c Oxidase Subunit I Gene-based Polymerase Chain Reaction}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {November 2017}, pages = {313-318}, type = {Original Article}, chapter = {313}, abstract = {

Background: Asini Corii Colla (ACC) (namely donkey hide gelatin, E{\textquoteright}jiao in Chinese) was one of the most valuable tonic traditional Chinese medicines which is an infallible remedy to promote hematopoiesis. It should be produced by fresh or dried donkey hide according to Chinese Pharmacopeia (2015 edition) with a long-time decoction, while as donkey and horse (or mule) all belong to equids so their hides or their hide gelatins are share much in common, that cause the difficult in distinguishing raw materials donkey hide from horse/mule hide for manufacturer, and the challenge in the quality evaluation of ACC for regulatory authority to identify the adulterated with horse hide. Objective: To establish an effective quality evaluation methods for ACC focused on the qualitative-based identification of the raw material{\textquoteright}s authenticity, mainly to identify the species origin of the gelatins. Materials and Methods: DNA extracted from (1) Raw materials (hides of donkey, horse, mule, bovine and pig); (2) Five hide-glues (bovine, pig, donkey, horse and mule hide-glue); (3) 11 batches of ACC commercial products made by different manufactures from local drug stores. Polymerase chain reaction (PCR) method with newly designed horse-specific primers I and primer pair II. Results: Use the primer pair I, a 234 bp target product could be amplified sensitively from the DNA sample of horse/mule adulterated commercial ACC products, though the DNA in commercial products is severely degraded. A 219 bp product could be amplified specifically from the DNA sample of horse/mule hide, while the results were all negative for the DNA templates of donkey hide, its gelatin and ACC products without adulteration. Conclusion: The developed PCR method based on primer I and II provide an effective approach to identify the species origin of highly processed product ACC (primer pair I) as well as to distinguish the raw material donkey hide (primer pair II), which might enlighten a new strategy to the Quality Evaluation of ACC.

}, keywords = {Asini Corii Colla, Equids, Polymerase chain reaction, Quality evaluation, Species identification}, doi = {10.4103/pr.pr_33_17}, author = {Hua-Li Zuo and Jie Zhao and Yi-Tao Wang and Zhi-Ning Xia and Yuan-Jia Hu and Feng-Qing Yang} }