@article {219, title = {Algae from Portuguese Coast Presented High Cytotoxicity and Antiproliferative Effects on an In vitro Model of Human Colorectal Cancer}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, pages = {24-30}, type = {Original Article}, chapter = {24}, abstract = {

Background: The marine environment has shown to be an interesting source of new antitumor agents, representing an important tool in cancer research. Objective: The aim of this study was to evaluate the antitumor activities of 12 algae from Peniche coast (Portugal) on an in vitro model of human colorectal cancer (Caco-2 cells). Materials and Methods: The antitumor potential was accessed by evaluating Caco-2 cell{\textquoteright}s viability and proliferation through the 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide and calcein-AM methods. Results: The dichloromethane extracts of Asparagopsis armata and Sphaerococcus coronopifolius induced the highest decrease on cell{\textquoteright}s viability (1 mg/mL; 24 h), 98.96\% {\textpm} 0.39\% and 98.08\% {\textpm} 0.89\%, respectively, followed by the methanolic extracts of S. coronopifolius (96.47\% {\textpm} 1.26\%) and A. armata (92.68\% {\textpm} 1.17\%). Regarding cell proliferation, the highest decrease of Caco-2 cell{\textquoteright}s proliferation (1 mg/mL; 24 h) was induced by the dichloromethane extract of A. armata (100\% {\textpm} 0.48\%), S. coronopifolius (99.04 {\textpm} 0.51\%), and Plocamium cartilagineum (95.05\% {\textpm} 1.19\%). The highest potency was shown by the dichloromethane extract of S. coronopifolius in both, cytotoxicity and antiproliferative tests, with an IC50 of 21.3 and 36.5 {\textmu}g/mL, respectively. Conclusion: The extracts of A. armata and S. coronopifolius are promising sources of new bioactive molecules with application in cancer therapeutics.

}, keywords = {Bioactive compounds, Caco-2 cells, Marine natural products, Red macroalgae, Seaweed}, doi = {10.4103/pr.pr_151_16}, author = {Celso Alves and Susete Pinteus and Ana Rodrigues and Andr{\'e} Horta and Rui Pedrosa} }