@article {182, title = {Chromatographic Profiles, Anti-inflammatory, and Cytotoxicity Potential of Extracts of Banisteriopsis pubipetala (A. Juss)}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {November,2019}, pages = {339-345}, type = {Original Article}, chapter = {339}, abstract = {

Background: Banisteriopsis pubipetala is a little-studied species belonging to the same genus of B. caapi, which stands out for the bioactive compounds known to be important in the degenerative diseases treatment. Objective: The objective is to analyze the anti-inflammatory and cytotoxic potential and chromatographic profile of the extracts of B. pubipetala. Materials and Methods: The investigation of the chromatographic profile was performed through high-performance liquid chromatography diode-array detector. Cell viability was determined by a quantitative colorimetric assay with 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide. In the pharmacological tests, interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), IL-10, and nitric oxide (NO) levels in cell culture supernatants were performed. Results: In the leaf were observed nine major compounds and in the stem five major compounds. The extracts of B. pubipetala demonstrated concentration-dependent behavior regarding cytotoxicity. The fraction in dichloromethane had inhibitory concentration of 50\% (IC50) = 67.39 μg/mL whereas the extract in ethyl acetate had IC50 = 103.37 μg/mL. The results showed that extracts significantly reduced the production of IL-6 and TNF-α by cells 3T3 cells, however increased the production of IL-10 and NO. Conclusion: The results of the tests indicate that the extracts of B. pubipetala evaluated have potential anti-inflammatory properties and may promote the regulation of inflammation levels.

}, keywords = {Banisteriopsis pubipetala, Interleukin-10, Interleukin-6, Malpighiaceae, Nitric oxide, Tumor necrosis factor-alpha.}, doi = {10.4103/pr.pr_71_19}, author = {Jeane Ferreira Leal de Freitas and Fl{\'a}via Dayrell Fran{\c c}a and M{\'\i}riam Martins Chaves and Elytania Veiga Menezes and Afr{\^a}nio Farias de Melo J{\'u}nior and Dario Alves de Oliveira and Clarice Avelar Almeida and Kamylla Santos Teixeira and Murilo Malveira Brand{\~a}o and Vanessa de Andrade Royo} } @article {973, title = {Cytotoxic Action and Proliferation in vitro and Analgesic Activity in vivo of Resin from Hymenaea stigonocarpa}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {August 2019}, pages = {193-200}, type = {Original Article}, chapter = {193}, abstract = {

Background:Hymenaea stigonocarpa\ Mart. (Fabaceae\ ) is one of the plant species commonly found in Brazilian cerrado. It is popularly known as jatob{\'a}-do-cerrado. Phytochemical studies of different parts of the plant demonstrated the presence of sesquiterpenes, diterpenes, and oligosaccharides. The species produces an opaque, translucent, pale yellow, or almost white resin rich in labdan{\'o}ides compounds, widely used in folk medicine, but without proving its pharmacological actions.\ Objective:\ To analyze the\ H. stigonocarpa\ resin to confirm its pharmacological actions through specific tests for cicatrization, nociception, and cytotoxicity and to evaluate the chemical composition through the gas chromatography coupled to mass spectrometry (GC-MS).\ Materials and Methods:\ The cell proliferation/migration capacity was evaluated using the scratch assay method and the cell viability determined by the 3-[4,5-dimethylthiazol-2-yl]{\textendash}tetrazolium 2,5-diphenyl-bromide cytotoxicity assay. In the pharmacological tests were performed Abdominal Contortions induced by acetic acid and Nociceptive Response evaluated by the Formalin Test. GC-MS was used to trace the profile of the bioactive compounds of the resin.\ Results:\ It was possible to verify the presence of terpene compounds with the labdanos skeleton. Structures that revealed important pharmacological actions regarding healing, evidenced by the scratch assay test and nociceptive activity demonstrated by the animal tests. No cytotoxic activity was detected at the dosages tested.\ Conclusion:\ The results obtained confirm the pharmacological action of the species and can be used to elaborate on new therapies, which will increase the therapeutic arsenal available to the population.

}, doi = {10.4103/pr.pr_30_19}, author = {Keyla Laisa Araujo E Saldanha and Vanessa de Andrade Royo and Francine Souza Alves Da Fonseca and Elytania Veiga Menezes and Dario Alves de Oliveira and Afr{\^a}nio Farias de Melo J{\'u}nior and Kamylla Santos Teixeira and Murilo Malveira Brand{\~a}o and Marcel Nani Leite and Guilherme Ferreira Caetano and Marco Andrey Cipriani Frade} }