@article {1453, title = {Fabrication of Snail Mucin-Silk Fibroin Hydrogel Scaffold for Guided Tissue Regeneration: A Zebrafish Fin Model Study}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {927-933}, type = {Original Article}, chapter = {927}, abstract = {

Background: Scaffolds play a pivotal role in guiding tissue regeneration by providing a suitable environment for cell growth, migration, and differentiation. Among various materials, biopolymers such as silk fibroin and snail mucin have garnered attention due to their biocompatibility, natural origin, and potential to promote cellular activities. Objectives: In this study, we explore the fabrication of a novel Snail Mucin-Silk Fibroin hydrogel scaffold designed to enhance tissue regeneration. Materials and Methods: The hydrogel scaffold has been prepared by mixing 28\% w/v gelatin, 21\% w/v PVA in distilled water and 10\% HPMC, and heated to 40{\textordmasculine}C followed by addition of a snail mucin and silk fibroin at a lower temperature and freeze dried at 4{\textordmasculine}C for 12 hr. Results: The characterization tests showed the scaffold{\textquoteright}s favourable biological properties and morphology, such as biocompatibility, hemocompatibility, ability to retain moisture, and promotion of cellular activities essential for tissue repair. Histopathological analysis showed that the snail mucin-silk fibroin hydrogel scaffold significantly enhanced fin regeneration compared to the control groups. Conclusion: Thus, the snail mucin hydrogel used in this study showed better soft tissue regeneration potential and hence be used for various applications in regenerative medicine.

}, keywords = {Hydrogel, Regeneration, Silk Fibroin, Snail Mucin, Zebra Fish}, doi = {10.5530/pres.20252176}, author = {Sofia Sebastian and Bargavi Purushothaman and Pratibha Ramani} } @article {1445, title = {Thymoquinone-Loaded Zinc Nanoparticles Mitigate Inflammation and Inhibit Glioblastoma Progression: A Novel Therapeutic Approach}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {850-858}, type = {Original Article}, chapter = {850}, abstract = {

Background: Glioblastoma is the most aggressive and lethal primary brain tumour, characterized by rapid proliferation, high invasiveness, and resistance to conventional therapies. Chronic inflammation plays a crucial role in its progression, with pro-inflammatory cytokines contributing to tumour growth and therapy resistance. Nanotechnology based approaches, such as Thymoquinone derived Zinc Nanoparticles (TQ-ZnNPs) offer a promising strategy to enhance drug bioavailability and target inflammation driven glioblastoma more effectively. Objectives: Thymoquinone, a naturally occurring substance with anti-inflammatory and anticancer qualities, has drawbacks such as low bioavailability and solubility. To overcome these, TQ-ZnNPs were synthesized using nanotechnology. Materials and Methods: TQ-ZnNPs were synthesized using a green nanotechnology approach and characterized for their physicochemical properties through techniques like, Scanning Electron Microscopy (SEM), Fourier-Transform Infrared spectroscopy (FTIR), X-ray Diffraction (XRD) and Ultraviolet-visible (UV-vis) spectroscopy examination to verify their optical and structural characteristics. Glioblastoma cells were treated with Lipopolysaccharide to induce inflammation, followed by exposure to TQ-ZnNPs to assess their anti-inflammatory and anti-cancer effects. RT-PCR method was used to assess gene expression. Results: TQ-ZnNPs exhibited enhanced stability and bioavailability, significantly reducing oxidative stress and suppressing pro-inflammatory cytokine expression in glioblastoma cells. They effectively inhibited cell proliferation and induced apoptosis, suggesting potent anti-inflammatory and anti-cancer properties. These findings highlight the therapeutic potential of TQ-ZnNPs in targeting inflammation driven glioblastoma. Conclusion: TQ-ZnNPs demonstrate significant anti-inflammatory and anti-cancer potential, making them a promising nanotherapeutic approach for combating inflammation driven glioblastoma.

}, keywords = {Anti-inflammatory, Glioblastoma, Inflammation, lipopolysaccharide, Nanotechnology, Thymoquinone, Zinc nanoparticles}, doi = {10.5530/pres.20252143}, author = {Sofia Sebastian and Taniya Mary Martin and Meenakshi Sundaram Kishore Kumar} }