@article {1340, title = {Analytical Characterization of Achyranthes aspera L. Whole Plant}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {85-93}, type = {Original Article}, chapter = {85}, abstract = {

Background: The therapeutic properties of Achyranthes aspera have long been acknowledged and integrated into diverse traditional healthcare like Ayurvedic, Siddha, Unani and Naturopathic medicine for the management of various ailments. In ancient Indian systems of medicine such as Siddha and Ayurveda, the constituents found in this medicinal plant play a pivotal role in treating disorders. Both macro and micro elements are crucial components for plants to carry out their basic functions. Objectives: Consequently, the ash of Achyranthes aspera was analyzed chemically, with a focus on phytochemical and chromatographic analysis, along with spectral characterization of the entire plant, to determine the presence of inorganic constituents. Materials and Methods: Standard methods were followed for all physicochemical parameters. Chromatographic characterization was carried out using High Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC). Additionally, elemental characterization was performed using different instruments such as Energy Dispersive X-ray analysis (EDX), X-ray Fluorescence (XRF), Powder X-ray Diffraction (PXRD), X-ray Photoelectron Spectroscopy (XPS) and Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) to investigate the minerals present in the ash of Achyranthes aspera. Results: The HPTLC photo documentation revealed the presence of five spots at 254 nm and eight spots at 366 nm. Vanillin sulfuric acid reagent derivatization resulted in the appearance of 9 spots under white light. Furthermore, the HPLC chromatogram showed the separation of 13 peaks at 366 nm. The evaluation techniques also detected various elements such as Carbon (C), Oxygen (O), Sodium (Na), Magnesium (Mg), Aluminium (Al), Silicon (Si), Phosphorus (O), sulphur (S), Chlorine (Cl), potassium (K), Calcium (Ca), Manganese (Mn), Chromium (Cr), Iron (Fe) and Zinc (Zn). Conclusion: The analysis primarily focuses on quality control criteria and the mineral composition of the plant.

}, keywords = {Achyranthes aspera, ICP-OES, Nayurivi, PXRD, SEM-EDX, XPS, XRF}, doi = {10.5530/pres.20251928}, author = {Achintya Kumar Mandal and Saravanan Meenakshisundaram and Subashini Subramanian and Sujith Thatipelli and Rajesh Chandran and Shakila Ramachandran} } @article {1199, title = {Chemical Characterization of Two Botanicals from Genus Alternanthera - A. brasiliana (L.) Kuntze and A. paronychioides A. St.-Hil}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {42-50}, type = {Original Article}, chapter = {42}, abstract = {

Background: Traditional medicine has become part and parcel of the present era for the maintaining and preventing of ailments. Alternanthera brasiliana and Alternanthera paronychioides (Amaranthaceae) are widely used in traditional medicine. Ab is widely familiar as penicillin in Brazil. Ap is known to treat gout, hyperuricemia, rheumatic arthritis, nephritis etc. as folk medicine. The present study aims to compare standardization profiles for A. brasiliana and A. paronychioides. Materials and Methods: Sample Ab and Ap were collected and authenticated. Authenticated samples were subjected to powder microscopy, physico-chemical, phytochemical, HPTLC and HPLC fingerprint and PXRD analysis. Results: Powder microscopic investigations revealed the characteristic features for identification. Physico-chemical investigation revealed the slightly acidic nature of both plants. The phytochemical test showed the existence of phenol, terpenoids and steroids as major secondary metabolites in both species. Photo documentation, fingerprints and spectral comparison by HPTLC and fingerprints by HPLC validate the existence of similar compounds in both Ab and Ap. PXRD analysis revealed the variance of elements present in both species. Conclusion: Comparative physico-chemical, phytochemical and HPLC, HPTLC, and P-XRD instrumental analysis of A. brasiliana and A. paronychioides provides distinct features for identification.

}, keywords = {Amaranthaceae, chromatographic fingerprinting, Comparative standardization, HPLC, HPTLC, PXRD.}, doi = {10.5530/pres.16.1.6}, author = {Achintya Kumar Mandal and Rajesh Allu and Rajesh Chandran and Divya Kallingil Gopi and Sunil Kumar Koppala Narayana and Radha Prakasam and Shakila Ramachandran} }