@article {1182, title = {Evaluation of Selectivity Index and Phytoconstituents Profile of Various Extracts from the Stem of Strychnos lucida R. Br. as Anti-malarial}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {October 2023}, pages = {733-750}, type = {Original Article}, chapter = {733}, abstract = {

Introduction: Strychnos lucida R. Br. or Songga was empirically used as an anti-malarial and immunostimulant in the Tetun tribe, Indonesia. However, almost all plants from the genus Strychnos contain the alkaloid toxic compound strychnine. Objectives: The aims of this study are to determine in vitro/in vivo anti-malarial activity, to analyze the selectivity index, and to measure the phytoconstituents of various extracts (water, ethanol, ethyl acetate, n-hexane) from S. lucida stem. Materials and Methods: In vitro anti-malarial study was conducted against Plasmodium falciparum 3D7-chloroquine-sensitive, and in vitro cytotoxic was performed against Monkey kidney Vero cell. Plasmodium berghei ANKA-chloroquine sensitive infected malaria mice were used as a model for evaluation of in vivo anti-plasmodial. Phytoconstituents profile was determined using Thermo scientific LC-HRMS, and the m/z data was analyzed using Compound Discoverer software with mzCloud MS/MS Library. Results: Water (W), Ethanol (E), and Ethyl Acetate (EA) extract exhibited more potential in vitro/in vivo anti-malarial activity than n-hexane (H) extract (IC50 2.48{\textpm}0.09; 2.45{\textpm}0.02; 2.90{\textpm}0.07; 7.64{\textpm}0.30 μg/mL, respectively). The selectivity index of water, ethanol, ethyl acetate, and n-hexane extract are 211.47, 78.46, 249.62, and 32.39, respectively). According to the LC-HRMS profile, water and ethanol extract mainly contain alkaloid and phenolic compounds. Ethyl acetate and n-hexane mostly contain terpenoids and fatty acids. Conclusion: According to the result, we conclude that ethanol and water extract from S. lucida R. Br. display potential anti-malarial. Alkaloids and phenolic compounds probably have the most contribution to their anti-malarial activity.

}, keywords = {Anti-malarial, LC-HRMS, Selectivity index, Strycnos lucida R. Br., Vero cell}, doi = {10.5530/pres.15.4.078}, author = {Uswatun Khasanah and Alvan Febrian Shalas and Bachtiar Rifai Pratita Ihsan and Lung Ayu Asti Wulandari and Nadela Cintya Nurtyas and Waril and Iin Alipiani and Ulfa Malihatus Sholiha} }