@article {1377, title = {A Unique Method for Simultaneous HPLC Analysis of Gallic Acid, Glycyrrhizic Acid and E-Guggulsterone in a Polyherbal Formulation}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {295-306}, type = {Original Article}, chapter = {295}, abstract = {

Background: The therapeutic effects of herbal medicines are exerted due to the presence of different phytoconstituents and the effects are further potentiated when compatible herbals are formulated together. High Performance Liquid Chromatography (HPLC) is an important analytical tool towards standardization of polyherbal formulation. Complex nature, co- existence with other constituents trace quantities of active phytoconstituents and their simultaneous estimation are challenges encountered in HPLC analysis of polyherbal formulation. Simultaneous estimation of active biomarkers in polyherbal formulation by HPLC provides a unique method for verifying the quality and efficacy of polyherbal formulation. Materials and Methods: Present research work is about inventing an unique, selective and sensitive HPLC (High Performance Liquid Chromatography) method for simultaneous estimation of Gallic acid, Glycyrrhizic acid and E-Guggulsterone from a polyherbal formulation containing thirteen Ethnobotanical herbs, reported to have Cardioprotective effects, marketed as Collasyn tablets by Dr. Palep Research Labs Ltd. in Indian market. Reverse-phase column (C18, 250 mM X4.6 mM i.d., 5μm) was used to analyze the marker compounds Gallic acid, Glycyrrhizic acid and E-Guggulsterone at λmax 224 nm. The mobile phase was composed of acetonitrile and 0.05\% OPA (Ortho-phosphoric acid) in water (50:50) with a flow rate of 1 mL/min. Results: A unique HPLC method is developed for simultaneous estimation of active markers-Gallic acid, Glycyrrhizic acid and E-Guggulsterone from the polyherbal formulation. Quantitative analysis shows Gallic acid, Glycyrrhizic acid and E-Guggulsterone to be 11.47\%, 1.05\% and 0.63\% respectively in the polyherbal formulation. The developed method is validated in accordance with the International Council for Harmonisation of Technical Requirements for Pharmaceuticals guidelines. The method shows good linearity in a relatively wide range of Concentration of 50-150 mcg/mL. The method is also found to be precise, accurate and robust. Conclusion: This unique and validated method for simultaneous estimation of cardioprotective biomarkers Gallic acid, Glycyrrhizic acid and E-Guggulsterone from polyherbal formulation, marketed as Collasyn tablets by Dr. Palep Research Labs Ltd. is important for its quality control. It helps in harnessing the complete plant potential for therapeutics. Thus, this approach to standardizing polyherbal formulations will be instrumental in verifying the quality and efficacy of the polyherbal extract. The simultaneous estimation of constituents from plants presents a significant advancement in phytochemical analysis, offering a comprehensive understanding of plant properties and their potential applications. The methodologies employed demonstrate high efficiency and accuracy, allowing for the effective quantification of multiple compounds in a single analytical run. This approach not only streamlines the process of phytochemical profiling but also enhances the ability to explore the therapeutic potentials of various plant species.

}, keywords = {Cardioprotective, HPLC, Method development, Method validation, Polyherbal formulation}, doi = {10.5530/pres.20252013}, author = {Bharati Gawade and Swati Patil} } @article {1055, title = {Tinospora cordifolia Stem Extract-mediated Green Synthesis of Selenium Nanoparticles and its Biological Applications}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {July 2022}, pages = {289-296}, type = {Original Article}, chapter = {289}, abstract = {

Background: According to a literature review Tinospora cordifolia possess alkaloids, tannins, flavonoids, glycosides, and steroids with anti-cancer potential. However, there is a lack of scientific evidence on the green synthesis of selenium nanoparticles from the stem of T. cordifolia and its biological activities. Objectives: This study is intended to synthesize Selenium Nanoparticles using T. cordifolia stem extract, characterise them, and evaluate them in-vitro for antioxidant and anticancer activity. Materials and Methods: The T. cordifolia Selenium Nanoparticles (TC-SeNPs) were synthesised by reducing selenious acid with T. cordifolia stem extract. TC-SeNPs were characterized by UV{\textendash}Vis, FTIR, XRD, FE-SEM, EDAX and ICP-AES, further evaluated for in-vitro antioxidant activity by DPPH free radical scavenging test and reducing power capacity. The MTT assay of TC-SeNP was carried out in-vitro in the human breast cancer cells line (MCF-7) to determine the anticancer activity. Results: The UV-spectroscopy of TC-SeNPs showed maximum absorbance at 285 nm, which is the characteristic feature of SeNP. According to DLS, SEM, and TEM images, the size of TC-SeNPs was between 100-200 nm and was stable with a Zeta potential of -23.9mV. XRD analysis confirmed the crystallinity of TC-SeNPs. Selenium in TC-SeNP was verified in EDAX analysis (63.36\% weight) and ICP-AES 68.08 {\textpm}12.2 μg/ml selenium content. The IC50 value 28.62 {\textpm} 0.63 μg/mL and EC50 (62.50 {\textpm} 1.21 μg/mL) values indicated that the TC-SeNPs possess significant antioxidant capacity. MTT assay showed TC-SeNPs were cytotoxic to MCF-7 cell with IC50 values (31.29 {\textpm} 0.22μg/mL), suggesting that TC-SeNPs display moderate cytotoxicity that could dose-dependently inhibit cell proliferation. Conclusion: Thus, experimental evidence provides insight into the successful synthesis of the selenium nanoparticle from the stem of T. cordifolia, its potential therapeutic value, and the prospect of developing an anti-cancer TC-SeNPs formulation.

}, keywords = {anticancer, Antioxidant, Green synthesis, Phytonanoparticles, Selenium nanoparticles, Tinospora cordifolia}, doi = {10.5530/pres.14.3.42}, author = {Abhijeet Puri and Swati Patil} }