@article {126, title = {Antiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {April 2021}, pages = {28-33}, type = {Original Article}, chapter = {28}, abstract = {

Objectives:\ The folk use of\ Doliocarpus dentatus\ for pain and inflammatory conditions led our group to evaluate the anti-inflammatory, antinociceptive, and antiarthritic effects of its ethanol extract from the leaves (EEDd) on mouse models.\ Results:\ Oral treatments with EEDd (100{\textendash}300 mg/kg) significantly inhibited the formalin-induced nociceptive and cold sensitivity, prevented acetic acid-induced nociceptive behavior, and prevented articular inflammation (including knee edema, leukocyte infiltration, and mechanical hyperalgesia) induced by zymosan. In the peritonitis model, betulinic acid (BA, 0.3{\textendash}30 mg/kg) and EEDd (300 mg/kg) significantly inhibited zymosan-induced leukocyte infiltration. In the complete Freund adjuvant (CFA) model, oral treatments with EEDd (100{\textendash}300 mg/kg) for 21 days significantly inhibited mechanical hyperalgesia, cold response, and edema. In the MTT viability assay, EEDd (3{\textendash}90 μg/mL) did not induce leukocytes cytotoxicity. cytotoxicity. Most models employed male and female\ Swiss\ mice or, for the CFA test, C57BL/6 mice.\ Conclusion:\ This study demonstrates that EEDd exhibited antinociceptive, antihyperalgesic, and antiarthritic potential in mice and BA contribute for the EEDd observed activities.

}, keywords = {Arthritis, Articular inflammation, Betulinic acid, Doliocarpus dentatus, Pain}, doi = {10.4103/pr.pr_79_20}, author = {Lidiane Schultz Branquinho and Maria Helena Verdan and Saulo Euclides Silva-Filho and Rodrigo Juliano Oliveira and Claudia Andrea Lima Cardoso and Arielle Cristina Arena and Candida Aparecida Leite Kassuya} }