@article {533, title = {Picroside I and Picroside II from Tissue Cultures of Picrorhiza kurroa}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {December 2017}, pages = {s53-s56}, type = {Original Article}, chapter = {s53}, abstract = {

Background: Picrorhiza kurroa (PK) belongs to Scrophulariaceae family and is a representative endemic, medicinal herb, widely distributed throughout the higher altitudes of alpine Himalayas from west to east, between 3000 and 4500 m above mean sea level. Objective: The objective of the present study is to assess the production of picroside I and picroside II from tissue cultures of PK. Materials and Methods: Auxiliary shoot tips of PK were incubated in Murashige and Skoog medium supplemented with indole-3-butyric acid and kinetin phytohormones. The callus produced was collected at different time intervals and was processed for extraction of picroside I and picroside II followed by thin layer chromatography and high-performance liquid chromatography HPLC analysis. Results: The maximum growth index was found to be 5.109 {\textpm} 0.159 at 16-week-old callus culture. The estimation of picroside-I and picroside-II was carried out by (HPLC) analysis; quantity of secondary metabolite found to be 16.37 {\textpm} 0.0007 mg/g for PK-I and 6.34 {\textpm} 0.0012 mg/g for PK-II. Conclusion: This is the first attempt to produce the Picroside-I and II in large amount by the tissue culture technique. It can be observed that the method of callus culture can be used in production of secondary metabolites Picroside-I and II from PK

}, keywords = {Picrorhiza kurroa, Picroside I, Picroside II, Tissue cultures}, doi = {10.4103/pr.pr_89_17}, author = {Yamjala Ganeshkumar and Ajmera Ramarao and Ciddi Veeresham} }