@article {431, title = {Validation of High-Performance Liquid Chromatography Method for Quantification of Costunolide Content in Laurus nobilis}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {October 2018}, pages = {422-425}, type = {Original Article}, chapter = {422}, abstract = {

Objective: To establish a method to determine the content of costunolide in Laurus nobilis leaves by high-performance liquid chromatography (HPLC). Materials and Methods: The separation was performed on a reversed-phase C18 column (100 {\r A}, 150 mm {\texttimes} 4.6 mm, 5 μm pore size) using a mobile phase composed of water:acetonitrile (40:60) at a flow rate of 1.0 ml/min. The detection was carried out on a ultraviolet detector at 210 nm. The developed method was validated according to the requirements for International Conference on Harmonisation guidelines. Results: The proposed method for costunolide was validated for linearity with excellent correlation coefficient (r2 \> 0.999). The relative standard deviation (RSD) is less than 1\% in precision (i.e repeatability and intermediate) of the method. The recovery rate for costunolide was within 100.54\%{\textendash}102.62\%. The limit of detection and limit of quantitation were 2.29 and 6.64 parts per million, respectively. Conclusion: The developed HPLC method is simple, rapid, precisely, accurately, and widely accepted and it is recommended for efficient assays in routine work.

}, keywords = {Costunolide, Laurus nobilis, Linearity, Precision, Recovery, Validation}, doi = {10.4103/pr.pr_31_18}, author = {Puttaswamy Naveen and Harakanahalli Basavegowda Lingaraju1 and Siddappa Chandrappa1 and Koretekera Shekharappa Priya, and Kodimule Shyam Prasad} }