@article {85, title = {Evaluation of Phytochemical Content of White Tea Clone 100 and Changes the Expression of Tumor Suppressor Genes on Colorectal Cancer Cell Line HCT116}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {August 2019}, pages = {224-229}, type = {Original Article}, chapter = {224}, abstract = {

Aim/Background: Colorectal carcinoma cancer is one of the main types of cancer with high death rate of patients, according to their non-healthy lifestyle. In this research, we evaluated inhibitory of white tea clone 100 extract on colorectal cancer cell line HCT-116, and its effectiveness in expression level of three tumor suppressor genes. Materials and Methods: Total polyphenolic content in all samples were measured using the Folin{\textendash}Ciocalteu method, and free radical adsorption investigated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the white tea extract inhibitory effect on HCT-116 cells. Results: Among different extraction methods, the white tea aqueous extracts produced the highest amount of polyphenols and DPPH radical scavenging activity (36.67 {\textpm} 0.54 mg gallic acid equivalent/g dry weight and 71.74\% {\textpm} 0.42\%, respectively). Cell survival analysis in the MTT assay indicated that aqueous white tea extract could reduce the viability of HCT-116 cells in 8, 16, and 24 h considerably, related to the concentration-dependent manner. The real-time polymerase chain reaction results indicated the significantly increased expression level (P \< 0.05) of tumor suppressor genes DCC, TGFBR2, and P53 in the concentration of 1000 μg/ml at 24 h. Conclusion: White tea aqueous extract because of having hydroxyl group in its structure at optimal concentration in all three times of experimental, could lead to a positive changes effect on gene expression in the tumor suppressor family.

}, keywords = {3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, HCT-116 cell line, Radical scavenging activity, Tumor suppressor genes, White tea}, doi = {10.4103/pr.pr_39_19}, author = {Fereydoon Bondarian and Asa Ebrahimi and Frouzandeh Mahjoubi and Eslam Majidi Hervan and Reza Azadi Gonbad} }