@article {73, title = {Chemical Profiling, Free Radical Scavenging and Anti-acetylcholinesterase Activities of Essential Oil from Curcuma caesia of Arunachal Pradesh, India}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {February 2020}, pages = {76-84}, type = {Original Article}, chapter = {76}, abstract = {

Background and Aim: The rhizomes of Curcuma caesia have traditional applications for pain healing and are an imperiled species of India. A study on investigation of in vitro anti-oxidant and acetylcholinesterase (AChE) inhibitory efficacy of essential oil from the rhizome part was carried out through extraction by hydrodistillation. Materials and Methods: The phyto components present in this volatile oil were identified by gas chromatography-mass spectrometric analysis and based on Spearman{\textquoteright}s correlation, the cooccurrence network of the 21 compounds were analyzed. In vitro anti-oxidant, anti-AChE activities were assessed with different concentrations of essential oil by spectrophotometric analysis, following the standard protocols. Results: The studies revealed that major compounds identified were Androsta-1,4-dien-3-one,17- (acetyloxy)-, (17.beta.)-Santanol acetate (16.11\%), Eucalyptol (12.98\%), Cycloprop[e]indene-1a,2(1H)-dicarboxaldehyde,3a,4,5,6,6a,6b-hexahydro- 5,5,6b-trimethyl, (1a. alpha., 3a. beta., 6a. beta., 6b. alpha) (8.96\%), methyl 7,12-octadecadienoate (6.75\%), and (+)-2-Bornanone (6.60\%). The major interactions of terpenoidal compounds with other compounds give a picture that terpenoids have a very significant role as anti-oxidants and cholinesterase inhibitors. The essential oil showed the total phenolic content of 26.72 {\textpm} 0.38 mg/g of gallic acid equivalent and total flavonoid content of 18.92 {\textpm} 0.27 mg/g of quercetin equivalent (QE). 2, 2-diphenyl-1-picrylhydrazyl and 2, 2{\textquoteright}-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid radical scavenging 50\% inhibitory concentration (IC50) values of the oil were 186.33 {\textpm} 0.46 {\textmu}g/mL and 109.41 {\textpm} 0.43 {\textmu}g/mL. The hydrogen peroxide and nitric oxide radical scavenging IC50s were 103.45 {\textpm} 0.58 {\textmu}g/mL and 190.55 {\textpm} 0.75 {\textmu}g/mL, respectively. The AChE inhibitory IC50 value was 156.33 {\textpm} 0.44 {\textmu}g/mL. Conclusion: The study demonstrated on essential oil extracted from rhizomes of C. caesia indigenous to Arunachal Pradesh, India, has moderate anti-oxidant property and would be a valuable non-toxic source for managing neurodegenerative diseases.

}, keywords = {Cholinergic, Ethnomedicinal, Hydrodistillation, Spectrophotometric, Terpenoid}, doi = {10.4103/pr.pr_84_19}, author = {Sudarshana Borah and Priyanka Sarkar and Hemanta Kumar Sharma} }