@article {632, title = {The Triterpenoid Fraction from Trichosanthes Dioica Root Exhibits in Vitro Antileishmanial Effect Against Leishmania Donovani Promastigotes}, journal = {Pharmacognosy Research}, volume = {5}, year = {2013}, month = {April 2013}, pages = { 109-112}, type = {Original Article }, chapter = {109}, abstract = {

Background:Trichosanthes dioica\ Roxb. (Cucurbitaceae), called pointed gourd in English is a dioecious climber found wild throughout the plains of the Indian subcontinent and traditionally used in India for several medicinal purposes.\ Objective:\ The present study was aimed at the evaluation of\ in vitro\ antileishmanial effect of triterpenoid fraction from\ T. dioica\ root (CETD).\ Materials and Methods:\ The antileishmanial activity of CETD was evaluated against\ Leishmania donovani\ (strain MHOM/IN/83/AG83)) promastigotes by\ in vitro\ promastigote cell toxicity assay by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide). Potassium antimonyl tartrate was used as reference.\ Results:\ Here, CETD markedly inhibited the growth of\ L. donovani\ promastigotes\ in vitro\ in a concentration dependent manner and demonstrated IC\ 50\ value of 18.75 μg/ml. The reference drug potassium antimonyl tartrate exhibited IC\ 50\ of 7.52 μg/ml.\ Conclusion:\ From the present study it can be inferred that the triterpenoid fraction of\ T. dioica\ root exhibited remarkable antileishmanial activity against\ Leishmania donovani\ promastigotes\ in vitro.

}, keywords = {Antileishmanial, Cucurbitacins, Leishmania Donovani, Ppromastigotes, Root, Trichosanthes Dioica}, doi = {10.4103/0974-8490.110540}, author = {Sanjib Bhattacharya and Moulisha Biswas and Pallab K Haldar} }