@article {494, title = {Evaluation of Skin Anti-aging Potential of Citrus reticulata Blanco Peel}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {May 2016}, pages = {160-168}, type = {Original Article}, chapter = {160}, abstract = {

Background: The peel of Citrus reticulata Blanco is traditionally used as tonic, stomachic, astringent, and carminative. It is also useful in skin care. Objective: To study the anti-aging potential of alcoholic extracts of C. reticulata Blanco peel using in vitro antioxidant and anti-enzyme assays. Materials and Methods: Plant extracts were obtained by Soxhlation (CR HAE- Hot Alcoholic Extract of Citrus reticulata) and maceration method (CR CAE- Cold Alcoholic Extract of Citrus reticulata). Qualitative and quantitative phytochemical analysis was performed. Further, in vitro antioxidant, anti-enzyme, and gas chromatography-mass spectrometry (GC-MS) analyses were performed. Results: Total phenolic and flavonoid contents of CR HAE were found to be higher than CR CAE. EC50 value of CR HAE and CR CAE for 1,1-Diphenyl-2-picrylhydrazyl, Superoxide anion, and 2, 2{\textquoteright}-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assays were 250.33 {\textpm} 40.16 μg/ml and 254.73 {\textpm} 15.78 μg/ml, 221.27 {\textpm} 11.25 μg/ml and 354.20 {\textpm} 23.79 μg/ml, and 59.16 {\textpm} 2.17 μg/ml and 59.12 {\textpm} 6.21 μg/ml, respectively. Oxygen radical absorbance capacity values for CR HAE and CR CAE were found to be 1243 and 1063 μmoles 6-hydroxy-2,5,7,8-tetra methylchromane-2-carboxylic acid equivalent/g of substance, respectively. Anti-collagenase and anti-elastase activities were evaluated for both CR HAE and CR CAE. EC50 values of CR HAE and CR CAE for anti-collagenase and anti-elastase were 329.33 {\textpm} 6.38 μg/ml, 466.93 {\textpm} 8.04 μg/ml and 3.22 {\textpm} 0.24 mg/ml, 5.09 {\textpm} 0.30 mg/ml, respectively. CR HAE exhibited stronger anti-collagenase and anti-elastase activity than CR CAE. GC-MS analysis of CR HAE was carried out because CR HAE exhibited higher antioxidant and anti-enzyme potential than CR CAE. Conclusion: C. reticulata peel can be utilized in anti-wrinkle skin care formulations.

}, keywords = {1, 1-diphenyl-2-picrylhydrazyl, 2, 2{\textquoteright}-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), anti-wrinkle, elastase, Gas chromatography-mass spectrometry, oxygen radical absorbance capacity}, doi = {10.4103/0974-8490.182913}, author = {Vinita D. Apraj and Nancy S. Pandita} }