@article {341, title = {Protective Effect of Combretum Hypopilinum Diels: Root Bark Extract against CCl4-Induced Hepatotoxicity in Wistar Rats}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {July 2018}, pages = {325-331}, type = {Original Article}, chapter = {325}, abstract = {

Background: Combretum hypopilinum is used in Togolese traditional medicine to alleviate various ailments including hepatic disorders. Objective: This study evaluates the hepatoprotective activities of crude ethanolic extract of C. hypopilinum root bark (CECH) and its possible mechanism(s) of action in Wistar rats. Materials and methods: In vitro antioxidant potential was evaluated using DPPH, Ferric-reducing power and hydroxyl radical scavenging assays. Hepatoprotective activity was assessed using CCl4-induced acute hepatotoxicity in rats. Animals were pretreated orally with different doses of CECH (200 and 400 mg/kg) once daily for 7 consecutive days and were subjected to intraperitoneal single injection of CCl4 (1 mL/kg). Serum markers of hepatic damage, hepatic endogenous antioxidants, NO, TNF-α and lipid peroxidation contents were measured out. Results: The pretreatment with CECH significantly decreased levels of hepatic enzymes (p \< 0.05, p \< 0.01, p \< 0.001) and MDA in pretreated rats (p \< 0.05, p \< 0.01). CECH restored the decreased levels of hepatic antioxidants including SOD (p \< 0.01), CAT (p \< 0.05 at 400 mg/kg) and GSH (p \< 0.05 at 400 mg/kg) and significantly inhibited hepatic production of NO (p \< 0.05, p \< 0.01). as well as the level of TNF-α (p \< 0.001) in pretreated rats. Protective effects of CECH were confirmed by histopathological examinations. CECH exhibited antioxidant in vitro and contains also total phenol and flavonoid. Conclusion: This study suggests that CECH protected the liver from CCl4-induced hepatic damage possibly via antioxidant and anti-inflammatory mechanisms.

}, keywords = {Combretum hypopilinum, Hepatoprotective, Oxidative stress, Tumor necrosis factor-α}, doi = {10.4103/pr.pr_27_17}, author = {Kokou Idoh and Kossivi Dosseh and Tchazou Kpatcha and Amegnona Agbonon and Messanvi Gbeassor} }