@article {338, title = {Evaluation of AntiCancer Activity of Methanolic Extract of Hiptage benghalensis (L.) Kurz on Cancer Cell Lines}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {July 2018}, pages = {309-313}, type = {Original Article}, chapter = {309}, abstract = {

Aim and Background: This study aimed to study the anticancer effects of Hiptage benghalensis (L.) kurz using various human cancer cell cultures, in vitro using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. Materials and Methods: Human cervical carcinoma (HeLa) cells, human breast cancer (MCF-7) cells, and human neuroblastoma (IMR-32) cells were maintained in a 5\% CO2 incubator at 37{\textdegree}C. Different concentrations of an extract of H. benghalensis in serum-free culture medium were freshly prepared and used for cytotoxic activity using MTT assay activity. Results: Among the plant extract had revealed that, the greater percentage inhibition in all types of cancer cells in a dose-dependent manner using MTT assay. The concentration of the extract causing 50\% cell death values of methanolic extract of H. benghalensis were found to be 50.73, 47.90, and 53.76 μg/mL against HeLa, MCF-7, and IMR-32, respectively. Conclusion: H. benghalensis methanolic extract were showed increased percentage inhibition of MCF 7, HeLa, and IMR 32 cells using MTT assay.

}, keywords = {caspase-3, Cell viability, Hiptage benghalensis, Reactive oxygen species}, doi = {10.4103/pr.pr_102_17}, author = {Babu Rao Bhukya and Narsimha Reddy Yellu} }