@article {302, title = {High-temperature Condition Increases Lignanoid Biosynthesis of Schisandra chinensis Seeds via Reactive Oxygen Species}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {February,2019}, pages = {72-77}, type = {Original Article}, chapter = {72}, abstract = {

Introduction: The herbal medicine used in many countries came mainly from the wild in the past; now, declining yield resource and laborious gathering result in prevailing cultivated medicine, with a result of prevailing inferior quality of herbal medicine. The contents of major functional ingredients varies greatly in the fruits of Schisandra chinensis, a herbal medicine in many Asian countries. Materials and Methods: These fruits were placed at 20{\textdegree}C, 35{\textdegree}C, 45{\textdegree}C, and 55{\textdegree}C for 1{\textendash}6 days, respectively, covered with plastics to prevent cells from anhydration during treating. The contents of H2O2, phenylalanine, and lignanoids and activities of antioxidant enzymes and phenylalanine ammonia lyase (PAL) were monitored. Results: The fresh seeds were exposed to 35{\textdegree}C, 45{\textdegree}C, and 55{\textdegree}C for 1 week; the H2O2 was rose sharply at 1 day and then declined but still with a higher level. The superoxide dismutase, catalase, and peroxidase activities were lowered, with inefficient antioxidant capacity. The PAL activities had a certain degree of high-temperature tolerance, remained largely unchanged at 35{\textdegree}C, but reduced gradually as temperature increased. High temperature activated the glycolytic pathway and rose the phenylalanine contents, which increased sharply at 1 day for 35{\textdegree}C and 45{\textdegree}C and at the 2 days for the 55{\textdegree}C and then maintained a stable level with almost 1{\textendash}3 times than the 0 day. Conclusion: The increased phenylalanine as substrate accelerated the synthesis of lignanoids; the contents of five lignanoids were increased by as much as 31.2\%{\textendash}81.5\%, respectively.

}, keywords = {Environmental stress, Lignanoids, Phenylalanine, Schisandra chinensis, Secondary metabolism.}, doi = {10.4103/pr.pr_42_18}, author = {Guo Huimin and Wang Jiahui and Gao Huiru and Meng Xiangcai} }