@article {232, title = {Evaluation of Antithrombotic Activities of Solanum xanthocarpum and Tinospora cordifolia}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {February 2018}, pages = {98-103}, type = {Original Article}, chapter = {98}, abstract = {

Background: Solanum xanthocarpum and Tinospora cordifolia have been reported to exhibit anti-inflammatory, antiarthritic, antioxidant, antiallergic, and hepatoprotective activities. The origins of many of the currently available antithrombotic treatments are from natural products and natural sources. Objective: To investigate the antithrombotic activities of methanolic leaf extracts of S. xanthocarpum (SXME) and T. cordifolia (TCME). Materials and Methods: Antithrombotic activities were assessed by thrombin inhibition assay, thrombin generation assay, platelet adhesion assay on collagen-coated surface, and platelet PAC1-FITC binding by flow cytometry. Results: SXME significantly inhibited thrombin activity at 5{\textendash}20 mg/ml concentrations, whereas TCME inhibited thrombin activity at 500 {\textmu}g/ml{\textendash}5 mg/ml concentrations. Further, SXME inhibited thrombin generation at 2{\textendash}20 mg/ml concentrations, whereas TCME exhibited significant inhibition at 200 {\textmu}g/ml, suggesting that TCME has higher efficacy as compared to SXME. Moreover, SXME did not inhibit platelet adhesion on collagen-coated surface, whereas TCME inhibited platelet adhesion on collagen-coated surface at 5 mg/ml. Indomethacin showed significant inhibition in platelet adhesion at 300 {\textmu}M. Further, SXME inhibited thrombin-induced platelet activation (PAC1-FITC binding) significantly at 1 mg/ml by about 80\%, whereas TCME inhibited thrombin-induced platelet activation (PAC1-FITC binding) by about 40\% at 1 mg/ml. Conclusion: These results strongly suggested that SXME and TCME possess antithrombotic activities. However, further studies are essential to find out the active constituent responsible for antithrombotic effect.

}, keywords = {Collagen, Platelet, Solanum xanthocarpum, Thrombin, Thrombosis, Tinospora cordifolia}, doi = {10.4103/pr.pr_80_17}, author = {Onila Lugun and Sobharani Bhoi and Priyanka Kujur and D. Vinay Kumar and William R. Surin} }