@article {116, title = {Safety and Antioxidant Potential of Traditional Thai Poly-Herbal Tea {\textquotedblleft}Phy-Blica-D{\textquotedblright} Used as a Rejuvenation Formula}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {August 2019}, pages = {295-303}, type = {Original Article}, chapter = {295}, abstract = {

Background: The rising popularity of phytonutrient consumption may be due to a vast number of scientific studies that have revealed their health benefits; however, concerns regarding the medical safety of herbal-based products are increasing. Phy-Blica-O is Phyllanthus emblica-based herbal tea used in Thai traditional medicine as a rejuvenating remedy. However, its consumption has been limited due to its strong bitter taste with unpleasant odor. Objective: The objective of this study is to evaluate the safety and antioxidant potential of Phy-Blica-D, the modified formula of Phy-Blica-O which gave high sensory acceptability scores. Materials and Methods: Subacute toxicity studies of Phy-Blica-D infusion was conducted by repeated oral administration of the extract at doses of 5, 50, and 300 mg/kg/day in Sprague-Dawley rats. Results: The formula exhibited antioxidant activity with an IC50 of 0.243 {\textpm} 0.006, 0.486 {\textpm} 0.002 and 0.108 {\textpm} 0.004 mg/mL using 1,1-diphenyl-2-picrylhydrazyl, 2,20-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid and metal chelating assays, respectively. There was no significant treatment-related toxicity as indicated by clinical signs, body weight, food consumption, serum biochemical and hematological parameters, organ weight, and histopathological examination of the animals treated with Phy-Blica-D infusion. These results suggest that the oral no-observed-adverse-effectlevel of Phy-Blica-D extract is \>300 mg/kg body weight/day, or ~3.7 L/kg body weight/day for both sexes. The calculated human equivalent dose value is 48.39 mg/kg/day, or ~600 mL/kg body weight/day. There were no target organs affected. Conclusion: This study demonstrates that Phy-Blica-D infusion can be regarded as safe and could potentially be used as a functional ingredient to reduce oxidative stress in non-communicable diseases.

}, keywords = {Antioxidant activity, Folkloric infusion, Functional beverage, Phyllanthus emblica, Subacute toxicity study.}, doi = {10.4103/pr.pr_5_19}, author = {Acharaporn Issuriya and Nuntitporn Puangkeaw and Piyapong Choochana and Patcharawalai Jaisamut and Nongluk Kunworarath and Katesarin Maneenoon and Surasak Limsuwan and Thammarat Kaewmanee and Sasitorn Chusri} }