ArticlesAbstractPharmacognosy Research,2025,17,4,1241-1252.DOI:10.5530/pres.20252163Published:August 2025Type:Original ArticleAuthors:Raj Joshi, Santosh Mudakappagol, and Adithya Anil Author(s) affiliations:Raj Joshi, Santosh Mudakappagol*, Adithya Anil Final Year PG Scholar, Department of Shalya Tantra, Shri BMK Ayurveda Mahavidyalaya Postgraduate Studies and Research Centre, A Constituent unit of KLE Academy of Higher Education and Research Centre, Deemed to be University, Belagavi, Karnataka, INDIA. Abstract:Background: Ayurveda healthcare system holds considerable reputation in today’s era. Treatises of Ayurveda explained numerous formulations with their potential therapeutic benefits but their global acceptance is poor due to the lack of scientific validity. Bhadrikadi Ghrita is one such formulation indicated for Marmakshat Ruja. Thus, there is an obvious need to overcome this lacuna by subjecting these medicines to thorough tests in light of modern research about their pharmacological, pharmacokinetic, and toxicity standards. Aim: The present study employs Gas Chromatography-Mass Spectrometry (GC-MS) analysis to investigate pain-relieving Ayurvedic Ghee, Bhadrikadi Ghrita, aiming to establish correlations between its medicinal activity and the biomolecules it contains. Materials and Methods: Bhadrikadi Ghrita was prepared according to Ayurvedic standards and subjected to GC-MS analysis. The detected compounds were identified by comparing their mass spectra with standard libraries and previous literature. A functional classification of the bioactive compounds was conducted to understand their potential therapeutic implications. Results and Discussion: The resulting profile revealed the presence of diverse array of bioactive compounds, including fatty acids, terpenoids, phenolic derivatives, and sterols. Notable compounds included linoleic acid, oleic acid, squalene, and curcumene. These constituents are associated with anti-inflammatory, antioxidant, antimicrobial, and neuroprotective properties. Conclusion: The identification of these bioactive compounds supports the therapeutic potentials of Bhadrikadi Ghrita as an effective pain-relieving ghee in Marmakshat Ruja, warranting further pharmacological and clinical studies. Keywords:Bhadrikadi Ghrita, Bioactive Metabolites, GC-MS, Marmakshat Ruja, Phytochemical analysis, Traditional medicineView:PDF (1.88 MB) PDF Thumbnails Document Outline Search Document Find Toggle Sidebar Previous Next Page: Fullscreen Print Download Current View Zoom Out Zoom In Automatic Zoom Actual Size Fit Page Full Width 50% 75% 100% 125% 150% 200% More Information Less Information Close Click here to download the PDF file. Images Gas chromatography-mass spectroscopy chromatogram of acetone extract of bhadrikadi Ghrita KeywordsBhadrikadi GhritaBioactive MetabolitesGC-MSMarmakshat RujaPhytochemical analysisPhytochemical AnalysisTraditional medicineTraditional Medicine ‹ Green Synthesis and Characterization of Copper Oxide Nanoparticles Using Vitex negundo Linn-An in vitro study up Accelerated Stability Study of Powder Formulation Safoof Muqliyasa and Safoof Mulayyin ›