Antioxidant, Stimulating Telomerase and Anxiolytic Activities of Polysaccharide Isolated from Sargentodoxa cuneata

Jian Tang; Ping Zhou; Huifang Sun; Xiuquan Xu; Chongwei Wen; Haiping Xia

Antioxidant, Stimulating Telomerase and Anxiolytic Activities of Polysaccharide Isolated from Sargentodoxa cuneata

Articles

Abstract

Pharmacognosy Research,2025,17,3,1020-1028.

DOI:10.5530/pres.20252269

Published:June 2025

Type:Original Article

Authors:

Author(s) affiliations:

Jian Tang^1,2,*, Ping Zhou¹, Huifang Sun³, Xiuquan Xu^1,2, Chongwei Wen³, Haiping Xia⁴

¹School of Chinese Medicine, Bozhou University, Bozhou, CHINA.

²Key Laboratory of Chinese Medicine Materials Research of Anhui Higher Education Institutes, Bozhou University, Bozhou, CHINA.

³School of Pharmacy, Jiangsu University, Zhenjiang, CHINA.

⁴Department of Neurology, The Affiliated Fourth People's Hospital of Jiangsu University, Zhenjiang, CHINA.

Abstract:

Background: The classic anxiolytics have adverse effects in the treating anxiety disorders. Aim: To identify the anxiolytic polysaccharide from Sargentodoxa cuneata. Materials and Methods: The water-soluble Polysaccharide Fraction (PSC) was purified from S. cuneata, as well as its subfractions PSCA-1 and PSCB-1. The antioxidant effect of PSC against rotenone induced PC12 cell excitotoxicity, stimulating telomerase effect in HEK293T cell and anxiolytic effect on zebrafish were evaluated. Results: The pretreatment of PC12 with PSC obviously increased cell survival and SOD level. PSC also down-regulated the LDH release, ROS level and cell apoptosis rate. PSC promoted the 293T cell proliferation, associated with the mechanism of stimulating telomerase. Additionally, PSC alleviated the anxiety level in zebrafish induced by mCPP. Conclusion: These findings suggested that the PSC could possess the anxiolytic potential via antioxidant and telomerase activation.