Margaritaria Discoidea (Euphorbiaceae) Stem Bark Extract Attenuates Allergy and Freund's adjuvant-induced Arthritis in Rodents

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Abstract
Pharmacognosy Research,2014,6,2,163-171.
Published:March,2014
Type:Original Article
Authors:
Author(s) affiliations:

David D Obiri, Newman Osafo, Joshua Oppong-Sarfo, Jude K Prah

Department of Pharmacology, Faculty of Pharmacy and Pharm. Sciences, College of Health Sciences, KNUST, Kumasi, Ghana.

Abstract:

Background: Various parts of Margaritaria discoidea find use in traditional medicine in the treatment of pain and oedema. This study evaluated the anti-allergic, anti-inflammatory and anti-arthritic effects of a 70% (v/v) aqueous ethanol extract of the stem bark of Margaritaria discoidea, MDE in rodents. Materials and Methods: Systemic anaphylaxis was induced by the injection of compound 48/80 into mice and their survival rate was monitored to evaluate the anti-allergic action of the extract. The effect of MDE assessed on the maximal and total oedema responses in the mouse carrageenan-induced paw oedema was used to evaluate the anti-inflammatory action of the extract while the Freund's adjuvant-induced arthritis model was employed to study the anti-arthritic effects of MDE. Results: MDE dose-dependently increased the time for compound 48/80-induced mortality in mice. MDE suppressed the mean maximal swelling and the total paw swellings induced over 6 h in the carrageenan-induced paw oedema when administered either prophylactically or therapeutically. MDE caused a reduction in serum levels of TNFα and IL-6 and significantly suppressed Freund's adjuvant-induced arthritis. Conclusion: Margaritaria discoidea suppresses allergy and exhibits anti-inflammatory activity in mice. In addition it attenuates Freund's adjuvant-induced arthritis through a reduction in serum levels of TNFα and IL-6 in rats.

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C57BL/6 mice (25-30 g) were pre-treated for 1 h with MDE 30-300 mg kg-1. Compound 48/80 was injected (10 mg kg-1, i.p.) and mortality monitored for 1 h

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