Background: Glyphaea brevis (Spreng) Monachino (Tiliaceae) have traditional uses in the management of conditions characterized by infections, inflammatory disorders and oxidative stress. The paper aims to report the comparative data on the leaves and stem bark of Glyphaea brevis with respect to their antibacterial, anti-inflammatory and antioxidant effects. Materials and Methods: The antibacterial effects of the 70% ethanol extracts of the leaves and stem bark were determined using the agar well diffusion and micro dilution assays. The anti-inflammatory activity was assessed using the carrageenan-induced oedema model in 7-day old cockerels. Using the DPPH free radical scavenging, total antioxidant and total phenol content assays, the antioxidant potential of the extract was assessed. Results: The bark extract had the higher antibacterial effect against 6 of the 8 microorganisms used. Noteworthy are its activity against Bacillus subtilis and Enterococcus faecalis with lowest MIC value of 500 ΅g/mL respectively. In doses of 30, 100 and 300 mg/kg, both extracts reduced the carrageenan-induced oedema in 7-day old cockerels. Based on the ED50 values, both extracts demonstrated similar potencies (ED50 =21.00 mg/kg). The stem bark extract exhibited higher free radical scavenging activity (IC50 = 1.392 mg/mL) compared to the leaf extract (IC50 = 9.509 mg/mL). In the total phenol content, the bark extract showed higher content (15.91 mg/g of dry mass) compared to the leaf extract (2.68 mg/g dry mass). Both extracts demonstrated equal potencies in the total antioxidant capacity determinations (0.60 mg/g dry weight of extract). Conclusions: The results of this work provide scientific evidence for the traditional uses of Glyphaea brevis.