Anti-leukemia Activity of Methanolic Extracts of Lantana camara

Badakhshan Mahdi Pour; Sreenivasan Sasidharan; Rameshwar Naidu Jegathambigai; Ramanathan Surash

Anti-leukemia Activity of Methanolic Extracts of Lantana camara

Articles

Abstract

Pharmacognosy Research,2009,1,5,274-279.

DOI:

Published:January 2010

Type:Original Article

Authors:

Author(s) affiliations:

Badakhshan Mahdi Pour^1,*, Sreenivasan Sasidharan¹, Rameshwar Naidu Jegathambigai¹ and Ramanathan Surash²

¹Department of Biotechnology, Faculty of Applied Sciences, AIMST University, 08100 Semeling- Bedong, Kedah, MALAYSIA.

²Centre for Drug Research, University Science of Malaysia, 11800 Minden, Pulau Pinang. MALAYSIA.

Abstract:

Anticancer effect of Lantana camara’s root and leaf extracts against Jurkat leukemia cell line was investigated by MTT assay. These extracts had statistically similar antineoplastic property (root IC₅₀, 328.36 ± 53.08 μg/ml; leaf, 394.41 ± 99.73 μg/ml; p > 0.1, n = 3), averagely 1/10 times as activity as carboplatin (IC50 34.83 ± 3.60 μg/ml; p < 0.05, n = 3). Decreasing cytotoxicity at higher concentrations implied the existence of cytoprotective compounds. Morphological examinations indicated apoptosis induction as the mechanism of activity on Jurkat cells. In conclusion, L. camara’s root and leaf extracts might be subjects for further fractionation and identification to find new anticancer agents.

Keywords:anticancer, Cell Culture, Jurkat Cell Line, Lantana camara, MTT assay

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Images

Morphology of Jurkat Leukemia Cells after 72 h Treating with Various Concentrations of Leaf Extract of L. camara. A: Solvent control, B: 15.6, C: 62.5, D: 250 and E: 500 μg/ml; F: Triton 100X 1%, G: Negative Control. Density of formazan crystals (blue color stick-like objects) is nearly straight linked to cell viability. Magnification is 400×.

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