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ORIGINAL ARTICLE
Year : 2020  |  Volume : 12  |  Issue : 3  |  Page : 225-229

Effect of a thai folk recipe on phytochemical screening, antioxidant activities, and α-Glucosidase inhibition by different solvent extracts


1 Ph.D. Candidate in Health Sciences Program, Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand
2 Health Sciences, Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand
3 Thai Traditional Medicine Research Unit, Applied Thai Traditional Medicine, Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand

Correspondence Address:
Dr. Ampa Konsue
Thai Traditional Medicine Research Unit, Applied Thai Traditional Medicine, Faculty of Medicine, Mahasarakham University, Maha sarakham, 44000
Thailand
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_100_19

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Context: Thai traditional medicine (TTM) has been widely use to treatment of various diseases. Aims: The aims of this study were determined on phytochemical screening, antioxidations, and α-glucosidase inhibition by different solvent extractions. Materials and Methods: The five medicinal plants from a TTM recipe were extracted using aqueous, 50% ethanol and 95% ethanol. The phytochemical screening were determined on total phenolic (TPC) and flavonoid (TFC) contents. Their antioxidant activities were tested using by 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging assay, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. Glucose transferase mechanism was evaluated using by α-glucosidase inhibitory assay. Results: The results found that the recipe was ingredient with TPC and TFC. The aqueous extract (AE) (47.955 ± 0.256 mgGE/gExt) was significantly highest amount on TPC while, the ethanolic extract (EE) (1.988 ± 0.038 mgQE/gExt) was showed significantly highest amount on TFC. The antioxidations, DPPH, and the AE (IC50 =0.227 ± 0.005) were significantly more potent on free radical scavenging. ABTS+, AE (IC50 =0.148 ± 0.007), Hydro-ethanolic extract (HEE) (IC50 =0.161 ± 0.008), and EE (IC50 =0.151 ± 0.007) were not different on this method. FRAP, EE (12.681 ± 0.620 mgTE/gEt) were significantly more potent on cation radical reducing. The α-Glucosidase inhibitory activity, AE (IC50 =0.021 ± 0.002 mg/mL) and HEE (IC50 =0.076 ± 0.003 mg/mL) were significantly more potent on EE (IC50 =0.292 ± 0.010 mg/mL) and Acarbose® (IC50 =1.05 ± 0.110 mg/mL). Conclusion: The study confirms traditional use of a Thai folk herbal plants on antioxidation and α-glucosidase inhibition. The recipe was contained with also TPC and TFC might be more potential to antioxidant activities and anti-α-glucoxidase enzyme. Future study, we should be performed to clarify the mechanisms, major active compounds and in vivo.


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