|Year : 2020 | Volume
| Issue : 3 | Page : 212-218
Liquid chromatography high-resolution mass spectrometry analysis, phytochemical and biological study of two aizoaceae plants: A new kaempferol derivative from Trianthema portulacastrum L.
Hala Abuzaid1, Elham Amin2, Abeer Moawad3, Usama Ramadan Abdelmohsen4, Mona Hetta5, Rabab Mohammed3
1 Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef; Department of Pharmacy, Ministry of Health, Minia, Egypt
2 Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah, 51452, Kingdom of Saudi Arabia
3 Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Minia, Egypt
4 Department of Pharmacognosy, Faculty of Pharmacy, Minia University; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Minia, Egypt
5 Department of Pharmacognosy, Faculty of Pharmacy, Fayoum University, Fayoum, Egypt
|Date of Submission||21-Jan-2020|
|Date of Acceptance||08-May-2020|
|Date of Web Publication||14-Aug-2020|
Prof. Rabab Mohammed
Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Natural remedies used for the treatment of liver diseases have a major concernworldwide. Objectives: Evaluation of the cytotoxic potential of successive fractions of two Aizoaceae plants; Trianthema portulacastrum L. and Aizoon canariense L. against human hepatocellular carcinoma (HepG2) cell lines. Moreover, metabolomic profiling of the successive fractions of both plants was carried out. Materials and Methods: Cytotoxic activity of successive fractions of the two plants against hepatocellular carcinoma (HCC) HepG2 cell lines were evaluated using viability assay, whereas metabolomic profiling was carried out using Liquid Chromatography High-Resolution ElectroSpray Ionization Mass Spectrometry (LC-HR-ESI-MS). Results: Significant cytotoxic activity of the n-hexane and n-butanol extracts of A. canariense (24.7 ± 3.5 and 55.3 ± 4.9 μg/mL, respectively) is recorded. On the other hand, metabolomic profiling of both plants resulted in dereplication of 27 metabolites belonging to different chemical classes; for example, sterols, flavonoids, diterpenes, triterpenes, tetraterpenes, alkaloids, lignans, hydrocarbons, and nucleosides. Phytochemical study of the biologically active fractions resulted in the isolation of one new compound; kaempferol-3-O-(2''-O-β-D-glucopyranosyl)-6''-O-E-feruloyl-β-D-glucopyranosid (T1). Biological testing of the isolated compounds indicated significant activity of T1 against HCC (IC50 =7.19 ± 0.27 μg/mL). Conclusion: Phytochemicals isolated from T. portulacastrum L and A. canariense L. may be responsible for their cytotoxic activity against HCC HepG2 cell lines.
Keywords: Aizoon, cytotoxic activity, human hepatocellular carcinoma cell lines, liquid chromatography high-resolution ElectroSpray ionization mass spectrometry, Trianthema
|How to cite this article:|
Abuzaid H, Amin E, Moawad A, Abdelmohsen UR, Hetta M, Mohammed R. Liquid chromatography high-resolution mass spectrometry analysis, phytochemical and biological study of two aizoaceae plants: A new kaempferol derivative from Trianthema portulacastrum L. Phcog Res 2020;12:212-8
|How to cite this URL:|
Abuzaid H, Amin E, Moawad A, Abdelmohsen UR, Hetta M, Mohammed R. Liquid chromatography high-resolution mass spectrometry analysis, phytochemical and biological study of two aizoaceae plants: A new kaempferol derivative from Trianthema portulacastrum L. Phcog Res [serial online] 2020 [cited 2021 Apr 11];12:212-8. Available from: http://www.phcogres.com/text.asp?2020/12/3/212/292048
- Metabolomic profiling and evaluation of the cytotoxic potential of successive fractions of Trianthema portulacastrum L. and Aizoon canariense L. are performed. The cytotoxic activity is evaluated against human hepatocellular carcinoma (HepG2 cell lines). Kaempferol-3-O-(2´´-O-β-D-glucopyranosyl)-6´´-O-E-feruloyl-β-D-glucopyranosid is isolated as a new compound from Trianthema portulacastrum L. herb.
Abbreviations Used: DNP: Dictionary of natural products; HCC: Hepatocellular carcinoma; LC-HR-ESI-MS: Liquid chromatography high-resolution ElectroSpray ionization mass spectrometry; TOF: Time-of- flight; UPLC: Ultra performance liquid chromatography.
| Introduction|| |
After cardiovascular disease, cancer is the second leading cause of death amongst the non-communicable diseases. Hepatocellular carcinoma (HCC) is one of the most fatal malignancies in Egypt, and this may be due to the spread of hepatitis B and C infections, the overuse of pesticides or aflatoxin contamination, especially in rural areas. Chemotherapy, hormonal therapy, radiotherapy, or surgery used for the treatment of cancer, have many side effects. Natural remedies have a long history for the treatment of liver diseases, and medicinal plants and natural products are continuously used all over the world. The most prominent chemotherapeutics of plant origin, which were obtained either directly through isolation or derived from lead structures, are the indole alkaloids vincristine and vinblastine, podophyllotoxin derivatives etoposide and teniposide. Desert plants have effective defense systems that allow them to withstand the stressful conditions for survival; bioactive secondary metabolites are one of these mechanisms.
Family Aizoaceae which are commonly known as ice plant, carpet weeds, or stone plants includes about 130 genera and 1200 species. Many of Aizoaceae plants are reported for their antifungal, antibacterial,, acaricidal activity against Rhipicephalus annulatus and antioxidant activity.
Trianthema portulacastrum L. is an Indian medicinal plant that exhibits anti-hepatotoxic activity, anti-hepatocarcinogenic potential and chemopreventive activity against breast cancer. Ecdysone is a major constituent of T. portulacastrum L. in addition to leptorumol, trianthenol, 5,2'-dihydroxy-7-methoxy-6,8-dimethylflavone, 3-acetylaleuritolic acid, 3,4-dimethoxy cinnamic acid, 5-hydroxy-2-methoxybenzaldehyde, betacyanin, and p-methoxybenzoic acid.
Aizoon canariense L.(Aizoaceae) is a perennial plant distributed in North Africa, Canary Islands, Mediterranean, Arabian Peninsula, south Iran, Pakistan, and Afghanistan. Few studies concerning the secondary metabolite content and the biological potential of this plant have been previously reported.
This paper was designed to evaluate the cytotoxic potential of different fractions of two Aizoaceae plants. In addition, the study of the metabolic pattern of both plants using LCHRMS was conducted. Furthermore, isolation and identification of the major components of the active fractions using different chromatographic and spectroscopic techniques and evaluation of their cytotoxic potential against HepG2 cell lines were carried out.
| Materials and Methods|| |
Bruker Avance III 400 MHz with AEON Nitrogen-Free Magnet and BBFO Smart Probe (Bruker AG, Switzerlaznd) was used to record1 H and13 C NMR spectra 400 MHz and 100 MHz, respectively. Data acquisition and processing were performed using Topspin 3.1 Software. Liquid Chromatography High-Resolution Electrospray Ionization Mass Spectrometry (LC-HR-ESI-MS) metabolomic analyses was performed using an Acquity Ultra Performance LC system connected to a Synapt G2 HDMS quadrupole time-of-flight hybrid mass spectrometer (Waters, Milford, USA).
Entire herbs of T. portulacastrum L. and A. canariense L. were collected in the flowering stage in spring and summer 2015 and 2017, from Cairo-Suez Canal road, East desert, Egypt and identified by Prof. Dr. Abdel Haleem Abdel Motagaly, Agriculture Museum, Giza, Egypt. The voucher specimen's numbers (R-Trian-10) and (R-Aizo) were given to T. portulacastrum L. and A. canariense L., respectively, and deposited at the Botanical Herbarium, Agriculture Museum, Dokki, Egypt.
Air-dried powdered plant material of T. portulacastrum and A. canariense (1 kg each) was separately exhaustively extracted with 70% ethanol (3 L × 5 times). The extracts were then concentrated under reduced pressure at 45°C. The alcoholic extract residues (116 and 120 g) of T. portulacastrum and A. canariense, respectively, were subjected to sequential solvent partitioning using n-hexane (3 × 500 ml), ethyl acetate (4 × 300 ml) and n-butanol (3 × 200 ml). Each solvent fraction was dried under vacuum till dryness.
Cytotoxic activity testing
The human HCC (HepG2) were obtained from the Tissue Culture Unit at VACSERA. Trypan blue dye, dimethyl sulfoxide, and crystal violet were purchased from Sigma (St. Louis, Mo., USA). Fetal Bovine serum, HEPES buffer solution, RPMI-1640, DMEM, gentamycin, 0.25% Trypsin-EDTA, and L-glutamine were purchased from Lonza. Cell line propagation and cytotoxicity evaluation were performed as previously described.,
Metabolomic analysis of different extracts of Trianthema portulacastrum and Aizoon canariense
Metabolomic analysis of the different fractions (n-hexane, ethyl acetate, and n-butanol) of T. portulacastrum and A. canariense was performed according to Abdelmohsen et al. using analytical techniques of LC-HR-ESI-MS. Detection of the metabolites was performed using ESI MS in both the positive and negative modes. Data were processed and extracted using MZmine 2.20. Chromatogram builder and deconvolution followed the detection of mass ion peaks. The local minimum search algorithm was processed, and isotopes were recognized by the isotopic peaks grouper. An adduct and complex search were done. Then, the processed data set was submitted to peak identification and molecular formula prediction. Both +ve and −ve ionization mode data sets were dereplicated against METLIN and Dictionary of natural products (DNP) databases. Hits from the database were retrieved using Chem-Bio Finder version 13.
Chromatographic isolation of the main components of Trianthema portulacastrum extracts
n-Hexane fraction (21 g) was subjected to fractionation by Vacuum LC using silica gel for column (90 g, 25 cm × 3.5 cm) eluting with petroleum ether (pet. ether) with increasing increments of ethyl acetate (EtOAc) and collecting 100 ml fractions. Fractions were monitored using TLC and n-hexane–EtOAc (8:2) solvent system. Similar fractions were pooled together to give three main subfractions. Subfraction 1 (300 mg) was chromatographed on a silica gel column using pet. ether-EtOAc system in 1% increments to isolate compound T2 (50 mg). Subfraction 2, (1.250 g) was similarly re-chromatographed to give compounds T3 and T4 mixture 400 mg white powder. Subfraction 3 (1.4 g) was re-chromatographed using silica gel column eluting with dichloromethane-methanol (DCM-MeOH) with 1% increments to give compound T5 (15 mg).
The ethyl acetate fraction (3 g) was chromatographed over silica gel using gradient elution technique with EtOAc-MeOH solvent system followed by chromatographic isolation over Sephadex LH-20 column using MeOH as eluent to afford compound T6 (10 mg), compound T7 (20 mg) and compound T1 (20 mg).
Chromatographic isolation of the main components of Aizoon canariense extracts
n- Hexane fraction (12 g) was similarly chromatographed as hexane fraction of T. portulacastrum to give two main fractions. Fraction A (228 mg) was re-chromatographed over the silica gel column using pet. ether-EtOAc in 1% increment till 8% to give compound A8 (25 mg). Fraction B (260 mg) was similarly rechromatographed on silica gel column to give compound A9 and A10 (100 mg) as a mixture.
EtOAc (3 g) and n- butanol extracts (3 g) were pooled and chromatographed on silica gel column using DCM-MeOH. Subfractions 15–20 were pooled and chromatographed using Sephadex LH-20 column to yield compounds A11 (20 mg) and A12 (10 mg).
Acid hydrolysis of compound T1
Acid hydrolysis of compound T1, silylation of the produced sugar part and GC-MS analysis and identification were performed according to Tewtrakul et al.
| Results and Discussion|| |
Cytotoxic activity testing of different extracts of Trianthema portulacastrum L. and Aizoon canariense L. against human hepatocellular carcinoma cell lines
Herein, two plants belonging to family Aizoaceae were tested for cytotoxic activity against HCC HepG2 cell lines. Results reveal the higher activity of the ethanol extract of A. canariense L (IC50 =50.9 ± 6.3) when compared to the ethanolic extract of T. portulacastrum L (IC50 =84.6 ± 7.9) [Table 1]. Tracing the cytotoxic activity in the successive fractions of each species indicates greater activity for the n-hexane, EtOAc and n- butanol extracts of A. canariense (24.7 ± 3.5, 93.8 ± 8.7 and 55.3 ± 4.9, respectively) than similar successive fractions of T. portulacastrum L (103 ± 8.4, 207 ± 19.8 and >500, respectively) [Table 1]. Many reports have discussed the anticancer activity of Aizoaceae plants. T. portulacastrum afforded considerable chemoprevention against breast cancer, and Sesuvium portulacastrum against Ehrlich ascites carcinoma.
|Table 1: IC50 (μg/mL) of the extracts of Trianthema portulacastrum Land Aizoon canariense L. against human hepatocellular carcinoma cell lines|
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Metabolomic analysis of different extracts of Trianthema portulacastrum and Aizoon canariense
Previous studies reported the isolation of variable compounds from T. portulacastrum L. However, no reports were found discussing the metabolomics content of A. canariense. Herein, LC-HR-MS analysis for dereplication purposes was adopted for the identification of metabolites from different fractions of T. portulacastrum and A. canariense. The dereplication study of the metabolites against the DNP and METLIN databases resulted in the identification of 24 compounds from different extracts of T. portulacastrum and 12 compounds from successive extracts of A. canariense [Table 2]. Metabolites identified from different extracts represent different chemical skeletons such as; flavonoids, sterols, hydrocarbons, lignans, diterpenes, triterpenes, tetraterpenes, nucleosides and alkaloids, where flavonoids are the most predominant class in both plants. LC-HRMS profile of T. portulacastrum indicates the presence of ten flavonoids; (2', 3, 3',4'-Tetrahydroxychalcone-3'-methylether (2), sesuvioside c (6), leptorumol (7), 7,8-dimethoxyflavanone (10), 2', 5, 7-trihydroxyflavanone (12), 2',5-dihydroxy-7-methoxy-6,8-dimethylflavone (16), 2',4'-dihydroxychalcone 17, ferulic acid (19), astragalin (23), sesuvioside f (25), six sterols; stigmasta-5,22-diene-3, 7, 11-triol (1), stigmasta-5,25-diene-3,7-diol-3-ketone (3), β-sitosterol glycoside (13), 20-hydroxyecdysone (15), stigmast-7-en-3-ol (26) and β-sitosterol (27), three hydrocarbons; tridecane (20), 1-O-(6-deoxy-6-sulfoglucopyranosyl) glycerol-3-tetradecanoyl (22) and 1-eicosanol (24), one lignan; Apteniol B (5), one diterpene (18-Hydroxy-17-nor-3,16-aphidicolanedione (9), one triterpene; Cycloartanol (18), one tetraterpene; Trianthenol (21) and one alkaloid; 4'-O-Methylsceletenone (14) [Table 2] and [Figure 1].
|Table 2: The liquid chromatography-high-resolution-ESIMS Dereplication results of Trianthema portulacastrum L. and Aizoon canariense different fractions (n-hexane, ethyl acetate, n-butanol)|
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|Figure 1: Dereplicated metabolites from LC-HR-ESIMS analysis of Trianthema portulacastrum and Aizoon canariens different fractions|
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On the other hand, LC-HRMS profile of A. canariense showed the presence of four flavonoids; (Leptorumol , Isorhamnetin-7-(6-trans-feruloylglucoside) , 2',5-Dihydroxy-7-methoxy-6,8-dimethylflavone  and Sesuvioside F , three sterols; [stigmasta-5,22-diene-3, 7, 11-triol (1), Stigmasta-5,25-diene-3,7-diol-3-Ketone (3) and Stigmast-7-en-3-ol (26), one hydrocarbon; [1-Eicosanol (24), one glucide; 1, 2, 3-Butanetriol (4) one diterpene; 18-Hydroxy-17-nor-3,16-aphidicolanedione (9), one triterpene; Cycloartanol (18) and one nucleoside; Adenosine (11) [Table 2] and [Figure 1].
Structural elucidation of the isolated compounds
To isolate the main metabolites in the active fractions, different chromatographic procedures were adopted. Phytochemical investigation of hexane extract of T. portulacastrum L resulted in the isolation of four compounds (T2–T5), while hexane extract of A. canariense afforded three compounds (A8–A10). Meanwhile, the examination of EtOAc fraction of T. portulacastrum L. resulted in the isolation of compounds (T1, T6 and T7). TLC screening of EtOAc and n-butanol fractions of A. canariense showed the same spots. Accordingly, EtOAc and n-butanol fractions were pooled together and the pooled fractions of A. canariense yielded two compounds A11 and A12. The structure elucidation of the isolated compounds was performed, adopting different spectroscopic techniques. Among the isolated compounds; (T1) was isolated as a yellow powder with [α]D20 +121 (c 0.01, CHCl3). It displays UV spectrum similar to kaempferol-3-O-glycosides, UV (MeOH)λmax327, 267, 213. HRMS spectrum of compound (T1) showed a molecular ion peak at m/z 786.6860 from which the molecular formula C37H38O19 was deduced.13 C NMR spectrum displayed 37 signals, among which 12 are attributable to two sugar moieties, 15 due to 3-O-glycosylated kaempferol and 10 due to feruloyl moiety [Table 3]. The spectrum displayed two anomeric carbons at δC100 and 104.9, in addition to other signals characteristic for two glucopyranosyl units.1 H-NMR spectrum displayed doublets at δH4.76 and 5.05 attributable to two anomeric protons with coupling constant 7.6 Hz indicating β-configuration of both protons. Other signals at δH6.77 (1 H, s), 6.66 (2 H, m), 6.04 (d, J = 16 Hz), 7.32 (d, J = 16 Hz) and 3.77 (3H, s) characteristic for feruloyl moiety. The coupling constant of the two doublets at δH6.04 and 7.32 is calculated as 16 Hz, thus indicating trans configuration of feruloyl moiety [Table 2]. The important HMBC correlations are shown in [Figure 2]. HMBC spectrum displayed three important3JCH correlations. First is the correlation between the anomeric proton at δH5.05 (H-1”) and carbon signal at δC133.7, indicating the attachment of the first glucose unit to the hydroxylated carbon (C-3) of the flavonoid moiety. Second is the correlation between δH4.75 (H-1”) and carbon signal at δC83.4 (C-2”) that proves the attachment of the terminal glucose unit to C2” of the first glucose unit. This is further confirmed by the downfield shift of C2” together with the upfield shift of C-1”. The third significant correlation between δH4.47 (H-6”) and δC167.7 confirms the attachment of feruloyl moiety to C-6” of glucose unit. Acid hydrolysis of T1 followed by derivatization was done according to Tewtrakul et al. The sugar derivatives thus obtained are analyzed by GC. It shows a retention time of 22.26 min, identical with that of authentic D-glucose. Accordingly, (T1) is identified as kaempferol-3-O-(2''-O-β-D-glucopyranosyl)-6''-O-E-feruloyl-β-D-glucopyranosid.
Based Upon1 H and13 C NMR, mass spectral data and comparison with previously reported literature, compounds were identified as cycloartanol (T2), β-sitosterol (T3), stigmasterol (T4), β-sitosterol glucoside (T5), kaempferol-3-O-β-glucopyranoside (T6), 20-hydroxyecdyson (7), 1-eicosanol (A8), spinasterol (A9), 7-stigmastenol (A10), isorhamnetin 3-O-β-glucopyranoside (A11), and adenosine (A12).
Cytotoxic activity testing of the isolated compounds
Testing the isolated compounds for the cytotoxic activity against HepG2 cell lines indicated significant activity of the new compound (T1) (IC50 =7.19 ± 0.27 μg/mL). Moderate activity was observed for Isorhamnetin-3-O-glucopyranoside (A11), Astragalin (T6) and 20-hydroxyecdysone (T7) (IC50 =28.1 ± 2.7, 30.7 ± 2.3, and 76.5 ± 4.9 μg/mL, respectively). Isorhamnetin-3-O-glucopyranoside was previously reported to exhibit cytotoxic activity against MCF-7 breast cancer cell lines with IC50 =28.1 μg/mL,, while, Astragalin was stated to suppress the proliferation of HCC cells. In addition, weak activity is observed for β- sitosterol-3-O-glucoside (5) (IC50 =251 μg/mL) and no activity is observed with cycloartanol (T2), β-sitosterol + stigmasterol (3 + 4) mixture, 1-octadecanol (8), spinasterol + 7-sigmastenol (9 + 10) mixture and adenosine (12) against HepG2 cell lines [Table 4]. It is noteworthy that this is the first report for the isolation and evaluation of the cytotoxic potential of kaempferol-3-O-(2''-O-β-D-glucopyranosyl)-6''-O-E-feruloyl-β-D-glucopyranosid (T1).
|Table 4: IC50 (μg/mL) of isolated compounds of Trianthema portulacastrum Land A. canariense L. against human hepatocellular carcinoma cell lines|
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| Conclusion|| |
The present study reports the cytotoxic activity of two plants belonging to family Aizoaceae against HCC HepG2 cell lines. Results reveal the higher activity of the ethanol, n-hexane, EtOAc and n-butanol extracts of A. canariense L, when compared to similar extracts of T. portulacastrum L. Metabolomic analysis of successive fractions of both plants shows the presence of several chemical classes, among them flavonoids are the most prominent in both plants. Interestingly, testing the isolated compounds for the cytotoxic activity indicates the significant activity of the new compound kaempferol-3-O-(2''-O-β-D-glucopyranosyl)-6''-O-E-feruloyl-β-D-glucopyranosid.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Desai AG, Qazi GN, Ganju RK, El-Tamer M, Singh J, Saxena AK, et al
. Medicinal Plants and Cancer Chemoprevention. Curr Drug Metab 2014;9:581-91.
Saleh A, Amr S, Jillson IA, Hueiyu Wang J, Crowell N, Loffredo CA. Preventing hepatocellular carcinoma in Egypt: Results of a Pilot Health Education Intervention Study. BMC Res Notes 2015;8:384-91.
Ali MA, Abul Farah M, Al-Hemaid FM, Abou-Tarboush FM. In vitro
cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells. Genet Mol Res 2014;13:3981-90.
Vijayaalakshmi LG. Herbal remedy for liver Cancer – Review. J Pharm Sci Res 2015;7:21-4.
Arcamone F, Cassinelli G, Casazza AM. New antitumor drugs from plants. J Ethnopharmacol 1980;2:149-60.
Harlev E, Nevo E, Lansky EP, Ofir R, Bishayee A. Anticancer potential of aloes: Antioxidant, antiproliferative, and immunostimulatory attributes. Planta Medica 2012. p. 843-52.
Al-Farhan AH, Al-Turki TA, Basahy AY. Flora of Jizan Region. Final Report 2005;1:545.
Mohammed R, El-Hawary SS, Abo-Youssef AM. Biological investigation of some wild Aizoaceae and Chenopediaceae species growing in Egypt. J Nat Prod 2012;5:193-206.
El-amier YA, Haroun SA, El-shehaby OA, Al-hadithy ON. Antioxidant and Antimicrobial Properties of Some Wild Aizoaceae Species Growing in Egyptian Desert. J Environ Sci 2016;45:1-10.
Moawad A, Mohammed R, Arafa W, Iriti M. Biologically-guided isolation of acaricidal phytosterols: An in vitro
study against Rhipicephalus
ticks infesting cattle in Egypt. Eur J Med Plants Brazil 2017;18:1-9.
Ibtissem B, Abdelly C, Sfar S. Antioxidant and antibacterial properties of Mesembryanthemum crystallinum
and Carpobrotus edulis
extracts. Adv Chem Eng Sci 2012;2:359-65.
Sharmila Banu G, Kumar G, Murugesan AG. Effect of ethanolic leaf extract of Trianthema portulacastrum
L. on aflatoxin induced hepatic damage in rats. Indian J Clin Biochem 2009;24:414-8.
Bishayee A, Mandal A, Chatierjee M. Prevention of alcohol-carbon tetrachloride-induced signs of early hepatotoxicity in mice by Trianthema portulacastrum
L. Phytomedicine 1996;3:155-161.
Westman J. A review of cancer immunotherapies and nutritional therapies. J Cancer Sci Ther 2015;7:28.
Shivhare MK, Singour PK, Chaurasiya PK, Pawar RS. Trianthema portulacastrum
Linn. (Bishkhapra). Pharmacogn Rev 2012;6:132-140.
Freije A, Alkhuzai J, Al-Laith AA. Fatty acid composition of three medicinal plants from Bahrain: New potential sources of γ-linolenic acid and dihomo-γ-linolenic. Ind Crops Prod 2013;43:218-24.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Gomha SM, Riyadh SM, Mahmmoud EM, Elaasser MM. Synthesis and anticancer activity of arylazothiazoles and 1,3,4-thiodiazoles using chitosan-grafted-poly(4-vinylpyridine) as a novel copolymer basic catalyst. Chem Heterocycl Compd 2015;51:1030-8.
Abdelmohsen U, Cheng C, Viegelmann C, Zhang T, Grkovic T, Ahmed S, et al
. Dereplication strategies for targeted isolation of new antitrypanosomal actinosporins A and B from a marine sponge associated-actinokineospora sp. EG49. Mar Drugs. 2014;12:1220-44.
Raheem DJ, Tawfike AF, Abdelmohsen UR, Edrada-Ebel RA, Fitzsimmons-Thoss V. Application of metabolomics and molecular networking in investigating the chemical profile and antitrypanosomal activity of British bluebells (Hyacinthoides non-scripta). Sci Rep 2019;9:1-13.
Ewtrakul ST, Akamura NN, Attori MH, Ujiwara TF. Flavanone and Flavonol Glycosides from the Leaves of Thevetia peruviana
and Their HIV-1 Reverse Transcriptase and HIV-1 Integrase Inhibitory. Chem Pharm Bull 2002;50:630-5.
Bishayee A, Mandal A. Trianthema portulacastrum
Linn. exerts chemoprevention of 7,12-dimethylbenz(a)anthracene-induced mammary tumorigenesis in rats. Mutat Res - Fundam Mol Mech Mutagen 2014;768:107-18.
Sheela D, Kalavathy U, Sheela D, Kalavathy U. Anticancer activity of methanol extract of Sesuvium portulacastrum
L. whole plant against Ehrlich ascites
carcinoma (EAC). Indo Am J Pharm Sci 2017;4:4500-6.
Kamel MS, Mohamed KM, Hassanean HA. Acylated flavonoid glycosides from Bassia muricata
. Phytochemistry 2001;57:1259-62.
Berti G, Bottari F, Marsili A, Morelli I, Polvani M, Mandelbaum A. 31-Norcycloartanol and cycloartanol from polypodium vulgare. Tetrahedron Lett. 1967;8:125-30.
Lokadi Pierre L. Isolation and characterisation of stigmasterol and β -sitosterol from Odontonema strictum
(Acanthaceae). J Innov Pharm Biol Sci 2015;2:88-95.
Beninger CW, Hosfield GL. Flavonoid composition of three genotypes of dry bean (Phaseolus vulgaris
) differing in seedcoat color. J Amer Soc Hort Sci 1999;124:514-8.
Girault JP, Lafont R. The complete 1H-NMR assignment of ecdysone and 20-hydroxyecdysone. J Insect Physiol 1988;34:701-6.
Amin E, Moawad A, Hassan H. Biologically-guided isolation of leishmanicidal secondary metabolites from Euphorbia peplus
L. Saudi Pharm J 2017;25:236-40.
Kojima H, Sato N, Hatano A, Ogura H. Sterol glucosides from Prunella vulgaris
. Phytochemistry 1990 2018;29:2351-5.
Wei Y, Xie Q, Fisher D, Sutherland IA. Separation of patuletin-3-O-glucoside, astragalin, quercetin, kaempferol and isorhamnetin from Flaveria bidentis
(L.) Kuntze by elution-pump-out high-performance counter-current chromatography. J Chromatogr A 2011;1218:6206-11.
Ciuffreda P, Casati S, Manzocchi A. Spectral assignments and reference data complete1
C NMR spectral assignment of a-and b-adenosine, 2-deoxyadenosine and their acetate derivatives. Magn Reson Chem Magn Reson Chem 2007;45:781-4.
Tundis R, Loizzo MR, Bonesi M, Menichini F, Statti GA, Menichini F. In vitro
cytotoxic activity of Salsola oppositifolia
Desf. (Amaranthaceae) in a panel of tumour cell lines. Zeitschrift fur Naturforsch - Sect C J Biosci 2008;63:347-54.
Li W, Hao J, Zhang L, Cheng Z, Deng X, Shu G. Astragalin reduces hexokinase 2 through increasing miR-125b to inhibit the proliferation of hepatocellular carcinoma cells in vitro
and in vivo
. J Agric Food Chem 2017;65:5961-72.
Luo X, Ma Y, WU S, WU D. The chemical constituents of Amoora yuuanensis
. Acta Bot Sin 2001;43:426-30.
Mativandlela SPN, Muthivhi T, Kikuchi H, Oshima Y, Hamilton C, Hussein AA, et al
. Antimycobacterial flavonoids from the leaf extract of Galenia africana
. J Nat Prod. 2009;72:2169-71.
Kokdil G, Topcu G, Voelter W. Steroids and terpenoids from Ajuga relicta
. Z Naturforsch 2002;57:957-60.
IShikawa T, Ega YS, Itajima JK. Water-soluble constituents of Ajowan. Chem Pharm Bull 2001;49:840-44.
DellaGreca M, Di Marino C, Previtera L, Purcaro R, Zarrelli A. Apteniols A–F, oxyneolignans from the leaves of Aptenia cordifolia
. Tetrahedron 2005;61:11924-9.
Disadee W, Mahidol C, Sahakitpichan P, Sitthimonchai S, Ruchirawat S, Kanchanapoom T. Flavonol 3- O-robinobiosides and 3- O-(2″- O-α-rhamnopyranosyl)-robinobiosides from Sesuvium portulacastrum
. Tetrahedron 2011;67:4221-6.
Kokpol U, Wannachet-isara N, Tip-pyang S, Chavasiri W, Veerachato G, Simpson J, et al
. A c-methylflavone from Trianthema portulacastrum
. Phytochemistry. 1997;44:719-22.
Li W, Yan Luo Q, qiang Wu L, Xiao L. Two new flavonol glycosides from Sedum aizoon
L. Heterocycles. 2011;83:135-141.
Dai J, Hussain H, Dräger S, Schulzb B, Kurtán T, Pescitelli G, et al
. Metabolites from the Fungus Phoma sp. 7210, Associated with Aizoon canariense
. Nat Prod Commun 2010;5:1175-80.
Kemp MS, Burden RS, Brown C. A new naturally occurring flavanone from Tetragonia expansa
. Phytochemistry 1979;18:1765-6.
Said AAE, Attia EZ, Abdelmohsen UR, Fouad MA. Natural products potential of the genus Aptenia. J Adv Biomed Pharm Sci 2019;2:59-62.
Banerji A, Chintalwar GJ, Joshi NK, Chadha MS. Isolation of ecdysterone from indian plants. Phytochemistry 1971;10:2225-6.
Yamaki J, Nagulapalli Venkata KC, Mandal A, Bhattacharyya P, Bishayee A. Health-promoting and disease-preventive potential of Trianthema portulacastrum
Linn. (Gadabani)-An Indian medicinal and dietary plant. J Integr Med 2016;14:84-99.
Magwa ML, Gundidza M, Gweru N, Humphrey G. Chemical composition and biological activities of essential oil from the leaves of Sesuvium portulacastrum
Nawaz HR, Malik A, Ali MS. Trianthenol: An antifungal tetraterpenoid from Trianthema portulacastrum
(Aizoaceae). Phytochemistry. 2001;56:99-102.
Chin Y-W, Jung H-A, Liu Y, Su B-N, Castoro JA, Keller WJ, et al
. Anti-oxidant Constituents of the Roots and Stolons of Licorice (Glycyrrhiza glabra
). J Agric Food Chem 2007;55:4691-7.
[Figure 1], [Figure 2]
[Table 1], [Table 2], [Table 3], [Table 4]