Liquid chromatography high-resolution mass spectrometry analysis, phytochemical and biological study of two aizoaceae plants: A new kaempferol derivative from Trianthema portulacastrum L.
Hala Abuzaid1, Elham Amin2, Abeer Moawad3, Usama Ramadan Abdelmohsen4, Mona Hetta5, Rabab Mohammed3
1 Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef; Department of Pharmacy, Ministry of Health, Minia, Egypt
2 Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah, 51452, Kingdom of Saudi Arabia
3 Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Minia, Egypt
4 Department of Pharmacognosy, Faculty of Pharmacy, Minia University; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Minia, Egypt
5 Department of Pharmacognosy, Faculty of Pharmacy, Fayoum University, Fayoum, Egypt
Prof. Rabab Mohammed
Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514
Source of Support: None, Conflict of Interest: None
Background: Natural remedies used for the treatment of liver diseases have a major concernworldwide. Objectives: Evaluation of the cytotoxic potential of successive fractions of two Aizoaceae plants; Trianthema portulacastrum L. and Aizoon canariense L. against human hepatocellular carcinoma (HepG2) cell lines. Moreover, metabolomic profiling of the successive fractions of both plants was carried out. Materials and Methods: Cytotoxic activity of successive fractions of the two plants against hepatocellular carcinoma (HCC) HepG2 cell lines were evaluated using viability assay, whereas metabolomic profiling was carried out using Liquid Chromatography High-Resolution ElectroSpray Ionization Mass Spectrometry (LC-HR-ESI-MS). Results: Significant cytotoxic activity of the n-hexane and n-butanol extracts of A. canariense (24.7 ± 3.5 and 55.3 ± 4.9 μg/mL, respectively) is recorded. On the other hand, metabolomic profiling of both plants resulted in dereplication of 27 metabolites belonging to different chemical classes; for example, sterols, flavonoids, diterpenes, triterpenes, tetraterpenes, alkaloids, lignans, hydrocarbons, and nucleosides. Phytochemical study of the biologically active fractions resulted in the isolation of one new compound; kaempferol-3-O-(2''-O-β-D-glucopyranosyl)-6''-O-E-feruloyl-β-D-glucopyranosid (T1). Biological testing of the isolated compounds indicated significant activity of T1 against HCC (IC50 =7.19 ± 0.27 μg/mL). Conclusion: Phytochemicals isolated from T. portulacastrum L and A. canariense L. may be responsible for their cytotoxic activity against HCC HepG2 cell lines.