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Year : 2020  |  Volume : 12  |  Issue : 1  |  Page : 17-23

Vitamin D3, L-Arginine, L-Citrulline, and antioxidant supplementation enhances nitric oxide bioavailability and reduces oxidative stress in the vascular endothelium – Clinical implications for cardiovascular system

Department of Chemistry and Biochemistry, Nanomedical Research Laboratories, Ohio University, Athens, Ohio, USA

Correspondence Address:
Prof. Tadeusz Malinski
Nanomedical Research Laboratories, Ohio University, 350 West State Street, Athens, Ohio
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pr.pr_79_19

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Background: Nitric oxide (NO) is a crucial signaling molecule which regulates the blood flow and prevents the adhesion of blood components to the vascular wall. A deficiency in bioavailable NO concentration is associated with the dysfunction of endothelial NO synthase (eNOS) and/or an increase in oxidative stress. The deficiency of bioavailable NO is a common denominator of several cardiovascular diseases, including diabetes, atherosclerosis, and hypertension. Materials and Methods: We used a nanomedical technology to elucidate the balance between bioavailable NO and oxidative stress (peroxynitrite ONOO) in human umbilical vein endothelial cells (HUVECs) treated with a supplement containing L-arginine, L-citrulline, Vitamin D3, and antioxidants. Nanosensors, with a diameter of 200–300 nm, are capable of measuring in situ NO and peroxynitrite (ONOO) concentrations produced by single endothelial cells. Results: The ratio of the concentration of cytoprotective NO [NO] to the concentration of cytotoxic peroxynitrite [ONOO] was used to estimate the efficiency of eNOS. HUVECs incubated with L-citrulline, L-arginine, and Vitamin D3increased the [NO]/[ONOO] ratio by 25%, while in the presence of antioxidants, the increase was 15%. The synergistic effect between the mix of L-arginine, L-citrulline, Vitamin D3, and antioxidants was a favorable increase of the overall [NO]/[ONOO] ratio by 50%. Conclusion: The findings of the study presented here clearly indicate that L-arginine, L-citrulline, and Vitamin D3can significantly alter the function of the endothelium and NO production, in a favorable manner, while pointedly reducing ONOO – the main component of oxidative stress. This effect can be significantly potentiated in the presence of antioxidants.

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