Oral intake of polyphenols of Chromolaena odorata: A perspective in peptic ulcer, thrombocytopenia, and heparin-induced bleeding diathesis in rodent model
Tania S Paul1, Biswadeep B Das2, Shakti P Ingale3, Niruta Killedar4, Kishori G Apte5
1 Satellite Lab of Venture Centre, APT Research Foundation, Hadapsar, Maharashtra, India
2 Deapartment of Genotoxicity, APT Testing and Research Pvt. Ltd., Hadapsar, Maharashtra, India
3 Department of Zoology, Annasaheb Magar Mahavidyalaya, Hadapsar, Maharashtra, India
4 Biology Division, IISER, Pashan, Pune, Maharashtra, India
5 Department of Pharmacogy, APT Research Foundation, Pune, Maharashtra, India
Dr. Tania S Paul
Satellite Lab of Venture Centre; C/O APT Research Foundation, S. No: 36/1/1, M. N. 199, Vadgaon Khurd, Sinhagad Road, Pune - 411 041, Maharashtra
Source of Support: None, Conflict of Interest: None
Objective: Bleeding disorder is of two types: external bleeding due to trauma, surgery, and internal bleeding which happens mainly due to ulcers and thrombocytopenia or use of excessive anticoagulants. Proven hemostatic activity of Chromolaena odorata (L) in bleeding and wound healing had encouraged to evaluate the efficacy of aqueous extract and spray-dried powder (SDP) on ulcers, thrombocytopenia, and heparin-induced bleeding diathesis. Materials and Methods: Internal bleeding in the form of lesions in empty stomach was induced by absolute ethanol. Busulfan is administered through intraperitoneal injection to produce thrombocytopenia in Wistar rats and heparin at 1000 IU/ml was injected intravenous to mice, and after 30 min, tail was truncated to qualitatively and quantitatively differentiate the bleeding diathesis in treated versus nontreated controls. Results: A significant reduction in the lesions was observed in treated rats as compared to disease control rats which was evident from ulcer index and histopathology data. Considerable protection and production of platelets were observed in the thrombocytopenia model which was drastically reduced in the Busulfan administered disease control. Both aqueous extract and SDP were successful in preventing blood loss in heparin-induced tail-bleeding model. Conclusion: The presence of polyphenols attributes to the crucial role of C. odorata in arresting internal bleeding from stomach ulcer, protecting the thrombocytes from destroying, and minimizing the bleeding diathesis in mice.
Abbreviations Used: SDP: Spray-dried powder, μL: Microliter, ml: Milliliter, nm: Nanometer, μg: Microgram, NAD: No abnormality detected, SD: Standard deviation.