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Year : 2018  |  Volume : 10  |  Issue : 3  |  Page : 325-331

Protective effect of Combretum Hypopilinum diels: Root bark extract against CCl4-Induced hepatotoxicity in wistar rats

Department of Physiology/Pharmacology, Faculty of Sciences, University of Lomé, Lomé, Togo

Correspondence Address:
Prof. Amegnona Agbonon
Department of Physiology/Pharmacology, Faculty of Sciences, University of Lomé, P.O. Box: 1515, Lomé
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pr.pr_27_17

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Background: Combretum hypopilinum is used in Togolese traditional medicine to alleviate various ailments including hepatic disorders. Objective: This study evaluates the hepatoprotective activities of crude ethanolic extract of C. hypopilinum root bark (CECH) and its possible mechanism(s) of action in Wistar rats. Materials and methods: In vitro antioxidant potential was evaluated using DPPH, Ferric-reducing power and hydroxyl radical scavenging assays. Hepatoprotective activity was assessed using CCl4-induced acute hepatotoxicity in rats. Animals were pretreated orally with different doses of CECH (200 and 400 mg/kg) once daily for 7 consecutive days and were subjected to intraperitoneal single injection of CCl4 (1 mL/kg). Serum markers of hepatic damage, hepatic endogenous antioxidants, NO, TNF-α and lipid peroxidation contents were measured out. Results: The pretreatment with CECH significantly decreased levels of hepatic enzymes (p < 0.05, p < 0.01, p < 0.001) and MDA in pretreated rats (p < 0.05, p < 0.01). CECH restored the decreased levels of hepatic antioxidants including SOD (p < 0.01), CAT (p < 0.05 at 400 mg/kg) and GSH (p < 0.05 at 400 mg/kg) and significantly inhibited hepatic production of NO (p < 0.05, p < 0.01).as well as the level of TNF-α (p < 0.001) in pretreated rats. Protective effects of CECH were confirmed by histopathological examinations. CECH exhibited antioxidant in vitro and contains also total phenol and flavonoid. Conclusion: This study suggests that CECH protected the liver from CCl4-induced hepatic damage possibly via antioxidant and anti-inflammatory mechanisms. Abbreviations Used: CECH: Crude ethanolic extract of combretum hypopilinum root bark, CCl4: Carbon tetrachloride, DPPH: 2,2-Diphenyl-1-picrylhydrazyl, AlCl3: Aluminum chloride, ALT: Alanine aminotransferase, AST: Aspartate aminotransferase, LDH: Lactate dehydrogenase, ALP: Alkaline phosphatase, TC: Total cholesterol, TG: Triglycerides, TB: Total bilirubin, TP: Total protein, MDA: Malondialdehyde, SOD: Superoxide dismutase, CAT: Catalase, GSH: Glutathione, NO: Nitric oxide.

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